Analysis of patchclamp recordings: model-free multiscale methods and software

Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-pa...

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Detalles Bibliográficos
Autores principales: Pein, Florian, Eltzner, Benjamin, Munk, Axel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071803/
https://www.ncbi.nlm.nih.gov/pubmed/33837454
http://dx.doi.org/10.1007/s00249-021-01506-8
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author Pein, Florian
Eltzner, Benjamin
Munk, Axel
author_facet Pein, Florian
Eltzner, Benjamin
Munk, Axel
author_sort Pein, Florian
collection PubMed
description Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00249-021-01506-8.
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spelling pubmed-80718032021-05-05 Analysis of patchclamp recordings: model-free multiscale methods and software Pein, Florian Eltzner, Benjamin Munk, Axel Eur Biophys J Original Article Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00249-021-01506-8. Springer International Publishing 2021-04-09 2021 /pmc/articles/PMC8071803/ /pubmed/33837454 http://dx.doi.org/10.1007/s00249-021-01506-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Pein, Florian
Eltzner, Benjamin
Munk, Axel
Analysis of patchclamp recordings: model-free multiscale methods and software
title Analysis of patchclamp recordings: model-free multiscale methods and software
title_full Analysis of patchclamp recordings: model-free multiscale methods and software
title_fullStr Analysis of patchclamp recordings: model-free multiscale methods and software
title_full_unstemmed Analysis of patchclamp recordings: model-free multiscale methods and software
title_short Analysis of patchclamp recordings: model-free multiscale methods and software
title_sort analysis of patchclamp recordings: model-free multiscale methods and software
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071803/
https://www.ncbi.nlm.nih.gov/pubmed/33837454
http://dx.doi.org/10.1007/s00249-021-01506-8
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