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A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes
In this work, we present evidence for the formation of transient stalks in aligned multilamellar stacks of lipid membranes. Just above the phase transition from the fluid ([Formula: see text] ) lamellar phase to the rhombohedral phase (R), where lipid stalks crystallize on a super-lattice within the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071804/ https://www.ncbi.nlm.nih.gov/pubmed/33590276 http://dx.doi.org/10.1007/s00249-020-01493-2 |
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author | Scheu, Max Komorowski, Karlo Shen, Chen Salditt, Tim |
author_facet | Scheu, Max Komorowski, Karlo Shen, Chen Salditt, Tim |
author_sort | Scheu, Max |
collection | PubMed |
description | In this work, we present evidence for the formation of transient stalks in aligned multilamellar stacks of lipid membranes. Just above the phase transition from the fluid ([Formula: see text] ) lamellar phase to the rhombohedral phase (R), where lipid stalks crystallize on a super-lattice within the lipid bilayer stack, we observe a characteristic scattering pattern, which can be attributed to a correlated fluid of transient stalks. Excess (off-axis) diffuse scattering with a broad modulation around the position which later transforms to a sharp peak of the rhombohedral lattice, gives evidence for the stalk fluid forming as a pre-critical effect, reminiscent of critical phenomena in the vicinity of second-order phase transitions. Using high-resolution off-specular X-ray scattering and lineshape analysis we show that this pre-critical regime is accompanied by an anomalous elasticity behavior of the membrane stack, in particular an increase in inter-bilayer compressibility, i.e., a decrease in the compression modulus. |
format | Online Article Text |
id | pubmed-8071804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-80718042021-05-05 A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes Scheu, Max Komorowski, Karlo Shen, Chen Salditt, Tim Eur Biophys J Original Article In this work, we present evidence for the formation of transient stalks in aligned multilamellar stacks of lipid membranes. Just above the phase transition from the fluid ([Formula: see text] ) lamellar phase to the rhombohedral phase (R), where lipid stalks crystallize on a super-lattice within the lipid bilayer stack, we observe a characteristic scattering pattern, which can be attributed to a correlated fluid of transient stalks. Excess (off-axis) diffuse scattering with a broad modulation around the position which later transforms to a sharp peak of the rhombohedral lattice, gives evidence for the stalk fluid forming as a pre-critical effect, reminiscent of critical phenomena in the vicinity of second-order phase transitions. Using high-resolution off-specular X-ray scattering and lineshape analysis we show that this pre-critical regime is accompanied by an anomalous elasticity behavior of the membrane stack, in particular an increase in inter-bilayer compressibility, i.e., a decrease in the compression modulus. Springer International Publishing 2021-02-15 2021 /pmc/articles/PMC8071804/ /pubmed/33590276 http://dx.doi.org/10.1007/s00249-020-01493-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Scheu, Max Komorowski, Karlo Shen, Chen Salditt, Tim A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes |
title | A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes |
title_full | A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes |
title_fullStr | A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes |
title_full_unstemmed | A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes |
title_short | A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes |
title_sort | stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071804/ https://www.ncbi.nlm.nih.gov/pubmed/33590276 http://dx.doi.org/10.1007/s00249-020-01493-2 |
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