Cargando…

P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, and it has gradually become the main disease burden in the world. However, the pathogenesis of NAFLD is complex, involving such things as dyslipidemia, oxidative stress, inflammation, etc. This brings to the table a s...

Descripción completa

Detalles Bibliográficos
Autores principales: An, Jing, Cheng, Lijun, Yang, Liping, Song, Nali, Zhang, Ju, Ma, Kejian, Ma, Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071996/
https://www.ncbi.nlm.nih.gov/pubmed/33912056
http://dx.doi.org/10.3389/fphar.2021.646239
_version_ 1783683827856572416
author An, Jing
Cheng, Lijun
Yang, Liping
Song, Nali
Zhang, Ju
Ma, Kejian
Ma, Ji
author_facet An, Jing
Cheng, Lijun
Yang, Liping
Song, Nali
Zhang, Ju
Ma, Kejian
Ma, Ji
author_sort An, Jing
collection PubMed
description Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, and it has gradually become the main disease burden in the world. However, the pathogenesis of NAFLD is complex, involving such things as dyslipidemia, oxidative stress, inflammation, etc. This brings to the table a significant challenge for drug development, and there is still no drug approved by the FDA on the market to treat the disease. GAS and HBA are active ingredients of the orchidaceae plant gastrodia elata and have exhibit effects in ameliorating nervous system diseases caused by oxidative stress. HBA is a metabolite of GAS that could perform lipid regulation and improve oxidative stress on HCD-induced NAFLD larval zebrafish, as reported by previous studies; we therefore explored the role of HBA in lipid regulation and oxidative stress on HCD-induced NAFLD larval zebrafish in vivo and FFA-induced BRL-3A hepatocyte in vitro. The gene expression of lipogenesis, inflammation, and oxidative stress were measured to investigate the underlying mechanism of HBA, and the potential protein target of HBA was explored by immunofluorescence. Altogether, our data highlight the role of HBA in improving NAFLD by use of its lipid-lowering and anti-oxidative properties via the Nrf2/HO-1 signaling pathway, providing a potential therapeutic compound for NAFLD treatment.
format Online
Article
Text
id pubmed-8071996
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80719962021-04-27 P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway An, Jing Cheng, Lijun Yang, Liping Song, Nali Zhang, Ju Ma, Kejian Ma, Ji Front Pharmacol Pharmacology Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, and it has gradually become the main disease burden in the world. However, the pathogenesis of NAFLD is complex, involving such things as dyslipidemia, oxidative stress, inflammation, etc. This brings to the table a significant challenge for drug development, and there is still no drug approved by the FDA on the market to treat the disease. GAS and HBA are active ingredients of the orchidaceae plant gastrodia elata and have exhibit effects in ameliorating nervous system diseases caused by oxidative stress. HBA is a metabolite of GAS that could perform lipid regulation and improve oxidative stress on HCD-induced NAFLD larval zebrafish, as reported by previous studies; we therefore explored the role of HBA in lipid regulation and oxidative stress on HCD-induced NAFLD larval zebrafish in vivo and FFA-induced BRL-3A hepatocyte in vitro. The gene expression of lipogenesis, inflammation, and oxidative stress were measured to investigate the underlying mechanism of HBA, and the potential protein target of HBA was explored by immunofluorescence. Altogether, our data highlight the role of HBA in improving NAFLD by use of its lipid-lowering and anti-oxidative properties via the Nrf2/HO-1 signaling pathway, providing a potential therapeutic compound for NAFLD treatment. Frontiers Media S.A. 2021-04-12 /pmc/articles/PMC8071996/ /pubmed/33912056 http://dx.doi.org/10.3389/fphar.2021.646239 Text en Copyright © 2021 An, Cheng, Yang, Song, Zhang, Ma and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
An, Jing
Cheng, Lijun
Yang, Liping
Song, Nali
Zhang, Ju
Ma, Kejian
Ma, Ji
P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway
title P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway
title_full P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway
title_fullStr P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway
title_full_unstemmed P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway
title_short P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway
title_sort p- hydroxybenzyl alcohol alleviates oxidative stress in a nonalcoholic fatty liver disease larval zebrafish model and a brl-3a hepatocyte via the nrf2 pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071996/
https://www.ncbi.nlm.nih.gov/pubmed/33912056
http://dx.doi.org/10.3389/fphar.2021.646239
work_keys_str_mv AT anjing phydroxybenzylalcoholalleviatesoxidativestressinanonalcoholicfattyliverdiseaselarvalzebrafishmodelandabrl3ahepatocyteviathenrf2pathway
AT chenglijun phydroxybenzylalcoholalleviatesoxidativestressinanonalcoholicfattyliverdiseaselarvalzebrafishmodelandabrl3ahepatocyteviathenrf2pathway
AT yangliping phydroxybenzylalcoholalleviatesoxidativestressinanonalcoholicfattyliverdiseaselarvalzebrafishmodelandabrl3ahepatocyteviathenrf2pathway
AT songnali phydroxybenzylalcoholalleviatesoxidativestressinanonalcoholicfattyliverdiseaselarvalzebrafishmodelandabrl3ahepatocyteviathenrf2pathway
AT zhangju phydroxybenzylalcoholalleviatesoxidativestressinanonalcoholicfattyliverdiseaselarvalzebrafishmodelandabrl3ahepatocyteviathenrf2pathway
AT makejian phydroxybenzylalcoholalleviatesoxidativestressinanonalcoholicfattyliverdiseaselarvalzebrafishmodelandabrl3ahepatocyteviathenrf2pathway
AT maji phydroxybenzylalcoholalleviatesoxidativestressinanonalcoholicfattyliverdiseaselarvalzebrafishmodelandabrl3ahepatocyteviathenrf2pathway