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Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids

Total thyroidectomy as part of thyroid cancer treatment results in hypothyroidism requiring lifelong daily thyroid hormone replacement. Unbalanced hormone levels result in persistent complaints such as fatigue, constipation, and weight increase. Therefore, we aimed to investigate a patient-derived t...

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Autores principales: Ogundipe, Vivian M.L., Groen, Andries H., Hosper, Nynke, Nagle, Peter W.K., Hess, Julia, Faber, Hette, Jellema, Anne L., Baanstra, Mirjam, Links, Thera P., Unger, Kristian, Plukker, John T.M., Coppes, Rob P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072035/
https://www.ncbi.nlm.nih.gov/pubmed/33711265
http://dx.doi.org/10.1016/j.stemcr.2021.02.011
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author Ogundipe, Vivian M.L.
Groen, Andries H.
Hosper, Nynke
Nagle, Peter W.K.
Hess, Julia
Faber, Hette
Jellema, Anne L.
Baanstra, Mirjam
Links, Thera P.
Unger, Kristian
Plukker, John T.M.
Coppes, Rob P.
author_facet Ogundipe, Vivian M.L.
Groen, Andries H.
Hosper, Nynke
Nagle, Peter W.K.
Hess, Julia
Faber, Hette
Jellema, Anne L.
Baanstra, Mirjam
Links, Thera P.
Unger, Kristian
Plukker, John T.M.
Coppes, Rob P.
author_sort Ogundipe, Vivian M.L.
collection PubMed
description Total thyroidectomy as part of thyroid cancer treatment results in hypothyroidism requiring lifelong daily thyroid hormone replacement. Unbalanced hormone levels result in persistent complaints such as fatigue, constipation, and weight increase. Therefore, we aimed to investigate a patient-derived thyroid organoid model with the potential to regenerate the thyroid gland. Murine and human thyroid-derived cells were cultured as organoids capable of self-renewal and which expressed proliferation and putative stem cell and thyroid characteristics, without a change in the expression of thyroid tumor-related genes. These organoids formed thyroid-tissue-resembling structures in culture. (Xeno-)transplantation of 600,000 dispersed organoid cells underneath the kidney capsule of a hypothyroid mouse model resulted in the generation of hormone-producing thyroid-resembling follicles. This study provides evidence that thyroid-lineage-specific cells can form organoids that are able to self-renew and differentiate into functional thyroid tissue. Subsequent (xeno-)transplantation of these thyroid organoids demonstrates a proof of principle for functional miniature gland formation.
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spelling pubmed-80720352021-04-29 Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids Ogundipe, Vivian M.L. Groen, Andries H. Hosper, Nynke Nagle, Peter W.K. Hess, Julia Faber, Hette Jellema, Anne L. Baanstra, Mirjam Links, Thera P. Unger, Kristian Plukker, John T.M. Coppes, Rob P. Stem Cell Reports Article Total thyroidectomy as part of thyroid cancer treatment results in hypothyroidism requiring lifelong daily thyroid hormone replacement. Unbalanced hormone levels result in persistent complaints such as fatigue, constipation, and weight increase. Therefore, we aimed to investigate a patient-derived thyroid organoid model with the potential to regenerate the thyroid gland. Murine and human thyroid-derived cells were cultured as organoids capable of self-renewal and which expressed proliferation and putative stem cell and thyroid characteristics, without a change in the expression of thyroid tumor-related genes. These organoids formed thyroid-tissue-resembling structures in culture. (Xeno-)transplantation of 600,000 dispersed organoid cells underneath the kidney capsule of a hypothyroid mouse model resulted in the generation of hormone-producing thyroid-resembling follicles. This study provides evidence that thyroid-lineage-specific cells can form organoids that are able to self-renew and differentiate into functional thyroid tissue. Subsequent (xeno-)transplantation of these thyroid organoids demonstrates a proof of principle for functional miniature gland formation. Elsevier 2021-03-11 /pmc/articles/PMC8072035/ /pubmed/33711265 http://dx.doi.org/10.1016/j.stemcr.2021.02.011 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ogundipe, Vivian M.L.
Groen, Andries H.
Hosper, Nynke
Nagle, Peter W.K.
Hess, Julia
Faber, Hette
Jellema, Anne L.
Baanstra, Mirjam
Links, Thera P.
Unger, Kristian
Plukker, John T.M.
Coppes, Rob P.
Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids
title Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids
title_full Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids
title_fullStr Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids
title_full_unstemmed Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids
title_short Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids
title_sort generation and differentiation of adult tissue-derived human thyroid organoids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072035/
https://www.ncbi.nlm.nih.gov/pubmed/33711265
http://dx.doi.org/10.1016/j.stemcr.2021.02.011
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