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Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids
Total thyroidectomy as part of thyroid cancer treatment results in hypothyroidism requiring lifelong daily thyroid hormone replacement. Unbalanced hormone levels result in persistent complaints such as fatigue, constipation, and weight increase. Therefore, we aimed to investigate a patient-derived t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072035/ https://www.ncbi.nlm.nih.gov/pubmed/33711265 http://dx.doi.org/10.1016/j.stemcr.2021.02.011 |
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author | Ogundipe, Vivian M.L. Groen, Andries H. Hosper, Nynke Nagle, Peter W.K. Hess, Julia Faber, Hette Jellema, Anne L. Baanstra, Mirjam Links, Thera P. Unger, Kristian Plukker, John T.M. Coppes, Rob P. |
author_facet | Ogundipe, Vivian M.L. Groen, Andries H. Hosper, Nynke Nagle, Peter W.K. Hess, Julia Faber, Hette Jellema, Anne L. Baanstra, Mirjam Links, Thera P. Unger, Kristian Plukker, John T.M. Coppes, Rob P. |
author_sort | Ogundipe, Vivian M.L. |
collection | PubMed |
description | Total thyroidectomy as part of thyroid cancer treatment results in hypothyroidism requiring lifelong daily thyroid hormone replacement. Unbalanced hormone levels result in persistent complaints such as fatigue, constipation, and weight increase. Therefore, we aimed to investigate a patient-derived thyroid organoid model with the potential to regenerate the thyroid gland. Murine and human thyroid-derived cells were cultured as organoids capable of self-renewal and which expressed proliferation and putative stem cell and thyroid characteristics, without a change in the expression of thyroid tumor-related genes. These organoids formed thyroid-tissue-resembling structures in culture. (Xeno-)transplantation of 600,000 dispersed organoid cells underneath the kidney capsule of a hypothyroid mouse model resulted in the generation of hormone-producing thyroid-resembling follicles. This study provides evidence that thyroid-lineage-specific cells can form organoids that are able to self-renew and differentiate into functional thyroid tissue. Subsequent (xeno-)transplantation of these thyroid organoids demonstrates a proof of principle for functional miniature gland formation. |
format | Online Article Text |
id | pubmed-8072035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-80720352021-04-29 Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids Ogundipe, Vivian M.L. Groen, Andries H. Hosper, Nynke Nagle, Peter W.K. Hess, Julia Faber, Hette Jellema, Anne L. Baanstra, Mirjam Links, Thera P. Unger, Kristian Plukker, John T.M. Coppes, Rob P. Stem Cell Reports Article Total thyroidectomy as part of thyroid cancer treatment results in hypothyroidism requiring lifelong daily thyroid hormone replacement. Unbalanced hormone levels result in persistent complaints such as fatigue, constipation, and weight increase. Therefore, we aimed to investigate a patient-derived thyroid organoid model with the potential to regenerate the thyroid gland. Murine and human thyroid-derived cells were cultured as organoids capable of self-renewal and which expressed proliferation and putative stem cell and thyroid characteristics, without a change in the expression of thyroid tumor-related genes. These organoids formed thyroid-tissue-resembling structures in culture. (Xeno-)transplantation of 600,000 dispersed organoid cells underneath the kidney capsule of a hypothyroid mouse model resulted in the generation of hormone-producing thyroid-resembling follicles. This study provides evidence that thyroid-lineage-specific cells can form organoids that are able to self-renew and differentiate into functional thyroid tissue. Subsequent (xeno-)transplantation of these thyroid organoids demonstrates a proof of principle for functional miniature gland formation. Elsevier 2021-03-11 /pmc/articles/PMC8072035/ /pubmed/33711265 http://dx.doi.org/10.1016/j.stemcr.2021.02.011 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ogundipe, Vivian M.L. Groen, Andries H. Hosper, Nynke Nagle, Peter W.K. Hess, Julia Faber, Hette Jellema, Anne L. Baanstra, Mirjam Links, Thera P. Unger, Kristian Plukker, John T.M. Coppes, Rob P. Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids |
title | Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids |
title_full | Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids |
title_fullStr | Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids |
title_full_unstemmed | Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids |
title_short | Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids |
title_sort | generation and differentiation of adult tissue-derived human thyroid organoids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072035/ https://www.ncbi.nlm.nih.gov/pubmed/33711265 http://dx.doi.org/10.1016/j.stemcr.2021.02.011 |
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