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Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex
Intermediate progenitor cells (IPCs) are neocortical neuronal precursors. Although IPCs play crucial roles in corticogenesis, their molecular features remain largely unknown. In this study, we aimed to characterize the molecular profile of IPCs. We isolated TBR2-positive (+) IPCs and TBR2-negative (...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072132/ https://www.ncbi.nlm.nih.gov/pubmed/33798452 http://dx.doi.org/10.1016/j.stemcr.2021.03.008 |
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author | Ulmke, Pauline Antonie Sakib, M. Sadman Ditte, Peter Sokpor, Godwin Kerimoglu, Cemil Pham, Linh Xie, Yuanbin Mao, Xiaoyi Rosenbusch, Joachim Teichmann, Ulrike Nguyen, Huu Phuc Fischer, Andre Eichele, Gregor Staiger, Jochen F. Tuoc, Tran |
author_facet | Ulmke, Pauline Antonie Sakib, M. Sadman Ditte, Peter Sokpor, Godwin Kerimoglu, Cemil Pham, Linh Xie, Yuanbin Mao, Xiaoyi Rosenbusch, Joachim Teichmann, Ulrike Nguyen, Huu Phuc Fischer, Andre Eichele, Gregor Staiger, Jochen F. Tuoc, Tran |
author_sort | Ulmke, Pauline Antonie |
collection | PubMed |
description | Intermediate progenitor cells (IPCs) are neocortical neuronal precursors. Although IPCs play crucial roles in corticogenesis, their molecular features remain largely unknown. In this study, we aimed to characterize the molecular profile of IPCs. We isolated TBR2-positive (+) IPCs and TBR2-negative (−) cell populations in the developing mouse cortex. Comparative genome-wide gene expression analysis of TBR2(+) IPCs versus TBR2(−) cells revealed differences in key factors involved in chromatid segregation, cell-cycle regulation, transcriptional regulation, and cell signaling. Notably, mutation of many IPC genes in human has led to intellectual disability and caused a wide range of cortical malformations, including microcephaly and agenesis of corpus callosum. Loss-of-function experiments in cortex-specific mutants of Esco2, one of the novel IPC genes, demonstrate its critical role in IPC maintenance, and substantiate the identification of a central genetic determinant of IPC biogenesis. Our data provide novel molecular characteristics of IPCs in the developing mouse cortex. |
format | Online Article Text |
id | pubmed-8072132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-80721322021-04-29 Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex Ulmke, Pauline Antonie Sakib, M. Sadman Ditte, Peter Sokpor, Godwin Kerimoglu, Cemil Pham, Linh Xie, Yuanbin Mao, Xiaoyi Rosenbusch, Joachim Teichmann, Ulrike Nguyen, Huu Phuc Fischer, Andre Eichele, Gregor Staiger, Jochen F. Tuoc, Tran Stem Cell Reports Resource Intermediate progenitor cells (IPCs) are neocortical neuronal precursors. Although IPCs play crucial roles in corticogenesis, their molecular features remain largely unknown. In this study, we aimed to characterize the molecular profile of IPCs. We isolated TBR2-positive (+) IPCs and TBR2-negative (−) cell populations in the developing mouse cortex. Comparative genome-wide gene expression analysis of TBR2(+) IPCs versus TBR2(−) cells revealed differences in key factors involved in chromatid segregation, cell-cycle regulation, transcriptional regulation, and cell signaling. Notably, mutation of many IPC genes in human has led to intellectual disability and caused a wide range of cortical malformations, including microcephaly and agenesis of corpus callosum. Loss-of-function experiments in cortex-specific mutants of Esco2, one of the novel IPC genes, demonstrate its critical role in IPC maintenance, and substantiate the identification of a central genetic determinant of IPC biogenesis. Our data provide novel molecular characteristics of IPCs in the developing mouse cortex. Elsevier 2021-04-01 /pmc/articles/PMC8072132/ /pubmed/33798452 http://dx.doi.org/10.1016/j.stemcr.2021.03.008 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Resource Ulmke, Pauline Antonie Sakib, M. Sadman Ditte, Peter Sokpor, Godwin Kerimoglu, Cemil Pham, Linh Xie, Yuanbin Mao, Xiaoyi Rosenbusch, Joachim Teichmann, Ulrike Nguyen, Huu Phuc Fischer, Andre Eichele, Gregor Staiger, Jochen F. Tuoc, Tran Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex |
title | Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex |
title_full | Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex |
title_fullStr | Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex |
title_full_unstemmed | Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex |
title_short | Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex |
title_sort | molecular profiling reveals involvement of esco2 in intermediate progenitor cell maintenance in the developing mouse cortex |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072132/ https://www.ncbi.nlm.nih.gov/pubmed/33798452 http://dx.doi.org/10.1016/j.stemcr.2021.03.008 |
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