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Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Digital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected off a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electron...

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Autores principales: Lachetta, Mario, Sandmeyer, Hauke, Sandmeyer, Alice, Esch, Jan Schulte am, Huser, Thomas, Müller, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072202/
https://www.ncbi.nlm.nih.gov/pubmed/33896207
http://dx.doi.org/10.1098/rsta.2020.0147
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author Lachetta, Mario
Sandmeyer, Hauke
Sandmeyer, Alice
Esch, Jan Schulte am
Huser, Thomas
Müller, Marcel
author_facet Lachetta, Mario
Sandmeyer, Hauke
Sandmeyer, Alice
Esch, Jan Schulte am
Huser, Thomas
Müller, Marcel
author_sort Lachetta, Mario
collection PubMed
description Digital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected off a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video projectors, and scientific applications such as microscopy. High-end fluorescence microscopy systems typically employ laser light sources, which by their nature provide coherent excitation light. In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. The universal combination of DMDs and coherent light sources, especially when working with multiple different wavelengths, is unfortunately not straight forward. The substructure of the tilted micromirror array gives rise to a blazed grating, which has to be understood and which must be taken into account when designing a DMD-based illumination system. Here, we present a set of simulation frameworks that explore the use of DMDs in conjunction with coherent light sources, motivated by their application in SIM, but which are generalizable to other light patterning applications. This framework provides all the tools to explore and compute DMD-based diffraction effects and to simulate possible system alignment configurations computationally, which simplifies the system design process and provides guidance for setting up DMD-based microscopes. This article is part of the Theo Murphy meeting ‘Super-resolution structured illumination microscopy (part 1)’.
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spelling pubmed-80722022022-02-02 Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy Lachetta, Mario Sandmeyer, Hauke Sandmeyer, Alice Esch, Jan Schulte am Huser, Thomas Müller, Marcel Philos Trans A Math Phys Eng Sci Articles Digital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected off a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video projectors, and scientific applications such as microscopy. High-end fluorescence microscopy systems typically employ laser light sources, which by their nature provide coherent excitation light. In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. The universal combination of DMDs and coherent light sources, especially when working with multiple different wavelengths, is unfortunately not straight forward. The substructure of the tilted micromirror array gives rise to a blazed grating, which has to be understood and which must be taken into account when designing a DMD-based illumination system. Here, we present a set of simulation frameworks that explore the use of DMDs in conjunction with coherent light sources, motivated by their application in SIM, but which are generalizable to other light patterning applications. This framework provides all the tools to explore and compute DMD-based diffraction effects and to simulate possible system alignment configurations computationally, which simplifies the system design process and provides guidance for setting up DMD-based microscopes. This article is part of the Theo Murphy meeting ‘Super-resolution structured illumination microscopy (part 1)’. The Royal Society Publishing 2021-06-14 2021-04-26 /pmc/articles/PMC8072202/ /pubmed/33896207 http://dx.doi.org/10.1098/rsta.2020.0147 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Articles
Lachetta, Mario
Sandmeyer, Hauke
Sandmeyer, Alice
Esch, Jan Schulte am
Huser, Thomas
Müller, Marcel
Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy
title Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy
title_full Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy
title_fullStr Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy
title_full_unstemmed Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy
title_short Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy
title_sort simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072202/
https://www.ncbi.nlm.nih.gov/pubmed/33896207
http://dx.doi.org/10.1098/rsta.2020.0147
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