Cargando…

Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia

Avian influenza viruses (AIVs) are maintained in wild bird reservoirs, particularly in mallard ducks and other waterfowl. Novel evolutionary lineages of AIV that arise through genetic drift or reassortment can spread with wild bird migrations to new regions, infect a wide variety of resident bird sp...

Descripción completa

Detalles Bibliográficos
Autores principales: Gulyaeva, Marina, De Marco, Maria Alessandra, Kovalenko, Ganna, Bortz, Eric, Murashkina, Tatiana, Yurchenko, Kseniya, Facchini, Marzia, Delogu, Mauro, Sobolev, Ivan, Gadzhiev, Alimurad, Sharshov, Kirill, Shestopalov, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072542/
https://www.ncbi.nlm.nih.gov/pubmed/33920551
http://dx.doi.org/10.3390/microorganisms9040864
_version_ 1783683930820444160
author Gulyaeva, Marina
De Marco, Maria Alessandra
Kovalenko, Ganna
Bortz, Eric
Murashkina, Tatiana
Yurchenko, Kseniya
Facchini, Marzia
Delogu, Mauro
Sobolev, Ivan
Gadzhiev, Alimurad
Sharshov, Kirill
Shestopalov, Alexander
author_facet Gulyaeva, Marina
De Marco, Maria Alessandra
Kovalenko, Ganna
Bortz, Eric
Murashkina, Tatiana
Yurchenko, Kseniya
Facchini, Marzia
Delogu, Mauro
Sobolev, Ivan
Gadzhiev, Alimurad
Sharshov, Kirill
Shestopalov, Alexander
author_sort Gulyaeva, Marina
collection PubMed
description Avian influenza viruses (AIVs) are maintained in wild bird reservoirs, particularly in mallard ducks and other waterfowl. Novel evolutionary lineages of AIV that arise through genetic drift or reassortment can spread with wild bird migrations to new regions, infect a wide variety of resident bird species, and spillover to domestic poultry. The vast continental reservoir of AIVs in Eurasia harbors a wide diversity of influenza subtypes, including both highly pathogenic (HP) and low pathogenic (LP) H7 AIV. The Caspian Sea region is positioned at the intersection of major migratory flyways connecting Central Asia, Europe, the Black and Mediterranean Sea regions and Africa and holds a rich wetland and avian ecology. To understand genetic reservoirs present in the Caspian Sea region, we collected 559 cloacal swabs from Anseriformes and other species during the annual autumn migration periods in 2017 and 2018. We isolated two novel H7N3 LPAIV from mallard ducks whose H7 hemagglutinin (HA) gene was phylogenetically related to contemporaneous strains from distant Mongolia, and more closely Georgia and Ukraine, and predated the spread of this H7 LPAIV sublineage into East Asia in 2019. The N3 neuraminidase gene and internal genes were prototypical of AIV widely dispersed in wild bird reservoirs sampled along flyways connected to the Caspian region. The polymerase and nucleoprotein segments clustered with contemporaneous H5 HPAI (clade 2.3.4.4b) isolates, suggesting the wide dispersal of H7 LPAIV and the potential of this subtype for reassortment. These findings highlight the need for deeper surveillance of AIV in wild birds to better understand the extent of infection spread and evolution along spatial and temporal flyways in Eurasia.
format Online
Article
Text
id pubmed-8072542
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80725422021-04-27 Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia Gulyaeva, Marina De Marco, Maria Alessandra Kovalenko, Ganna Bortz, Eric Murashkina, Tatiana Yurchenko, Kseniya Facchini, Marzia Delogu, Mauro Sobolev, Ivan Gadzhiev, Alimurad Sharshov, Kirill Shestopalov, Alexander Microorganisms Communication Avian influenza viruses (AIVs) are maintained in wild bird reservoirs, particularly in mallard ducks and other waterfowl. Novel evolutionary lineages of AIV that arise through genetic drift or reassortment can spread with wild bird migrations to new regions, infect a wide variety of resident bird species, and spillover to domestic poultry. The vast continental reservoir of AIVs in Eurasia harbors a wide diversity of influenza subtypes, including both highly pathogenic (HP) and low pathogenic (LP) H7 AIV. The Caspian Sea region is positioned at the intersection of major migratory flyways connecting Central Asia, Europe, the Black and Mediterranean Sea regions and Africa and holds a rich wetland and avian ecology. To understand genetic reservoirs present in the Caspian Sea region, we collected 559 cloacal swabs from Anseriformes and other species during the annual autumn migration periods in 2017 and 2018. We isolated two novel H7N3 LPAIV from mallard ducks whose H7 hemagglutinin (HA) gene was phylogenetically related to contemporaneous strains from distant Mongolia, and more closely Georgia and Ukraine, and predated the spread of this H7 LPAIV sublineage into East Asia in 2019. The N3 neuraminidase gene and internal genes were prototypical of AIV widely dispersed in wild bird reservoirs sampled along flyways connected to the Caspian region. The polymerase and nucleoprotein segments clustered with contemporaneous H5 HPAI (clade 2.3.4.4b) isolates, suggesting the wide dispersal of H7 LPAIV and the potential of this subtype for reassortment. These findings highlight the need for deeper surveillance of AIV in wild birds to better understand the extent of infection spread and evolution along spatial and temporal flyways in Eurasia. MDPI 2021-04-17 /pmc/articles/PMC8072542/ /pubmed/33920551 http://dx.doi.org/10.3390/microorganisms9040864 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gulyaeva, Marina
De Marco, Maria Alessandra
Kovalenko, Ganna
Bortz, Eric
Murashkina, Tatiana
Yurchenko, Kseniya
Facchini, Marzia
Delogu, Mauro
Sobolev, Ivan
Gadzhiev, Alimurad
Sharshov, Kirill
Shestopalov, Alexander
Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia
title Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia
title_full Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia
title_fullStr Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia
title_full_unstemmed Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia
title_short Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia
title_sort biological properties and genetic characterization of novel low pathogenic h7n3 avian influenza viruses isolated from mallard ducks in the caspian region, dagestan, russia
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072542/
https://www.ncbi.nlm.nih.gov/pubmed/33920551
http://dx.doi.org/10.3390/microorganisms9040864
work_keys_str_mv AT gulyaevamarina biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT demarcomariaalessandra biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT kovalenkoganna biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT bortzeric biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT murashkinatatiana biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT yurchenkokseniya biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT facchinimarzia biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT delogumauro biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT sobolevivan biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT gadzhievalimurad biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT sharshovkirill biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia
AT shestopalovalexander biologicalpropertiesandgeneticcharacterizationofnovellowpathogenich7n3avianinfluenzavirusesisolatedfrommallardducksinthecaspianregiondagestanrussia