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Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review
The hair research field has seen great improvement in recent decades, with in vitro hair follicle (HF) models being extensively developed. However, due to the cellular complexity and number of various molecular interactions that must be coordinated, a fully functional in vitro model of HFs remains e...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072628/ https://www.ncbi.nlm.nih.gov/pubmed/33923738 http://dx.doi.org/10.3390/biomedicines9040435 |
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author | Žnidarič, Matej Žurga, Žan Michel Maver, Uroš |
author_facet | Žnidarič, Matej Žurga, Žan Michel Maver, Uroš |
author_sort | Žnidarič, Matej |
collection | PubMed |
description | The hair research field has seen great improvement in recent decades, with in vitro hair follicle (HF) models being extensively developed. However, due to the cellular complexity and number of various molecular interactions that must be coordinated, a fully functional in vitro model of HFs remains elusive. The most common bioengineering approach to grow HFs in vitro is to manipulate their features on cellular and molecular levels, with dermal papilla cells being the main focus. In this study, we focus on providing a better understanding of HFs in general and how they behave in vitro. The first part of the review presents skin morphology with an emphasis on HFs and hair loss. The remainder of the paper evaluates cells, materials, and methods of in vitro growth of HFs. Lastly, in vitro models and assays for evaluating the effects of active compounds on alopecia and hair growth are presented, with the final emphasis on applications of in vitro HFs in hair transplantation. Since the growth of in vitro HFs is a complicated procedure, there is still a great number of unanswered questions aimed at understanding the long-term cycling of HFs without losing inductivity. Incorporating other regions of HFs that lead to the successful formation of different hair classes remains a difficult challenge. |
format | Online Article Text |
id | pubmed-8072628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80726282021-04-27 Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review Žnidarič, Matej Žurga, Žan Michel Maver, Uroš Biomedicines Review The hair research field has seen great improvement in recent decades, with in vitro hair follicle (HF) models being extensively developed. However, due to the cellular complexity and number of various molecular interactions that must be coordinated, a fully functional in vitro model of HFs remains elusive. The most common bioengineering approach to grow HFs in vitro is to manipulate their features on cellular and molecular levels, with dermal papilla cells being the main focus. In this study, we focus on providing a better understanding of HFs in general and how they behave in vitro. The first part of the review presents skin morphology with an emphasis on HFs and hair loss. The remainder of the paper evaluates cells, materials, and methods of in vitro growth of HFs. Lastly, in vitro models and assays for evaluating the effects of active compounds on alopecia and hair growth are presented, with the final emphasis on applications of in vitro HFs in hair transplantation. Since the growth of in vitro HFs is a complicated procedure, there is still a great number of unanswered questions aimed at understanding the long-term cycling of HFs without losing inductivity. Incorporating other regions of HFs that lead to the successful formation of different hair classes remains a difficult challenge. MDPI 2021-04-16 /pmc/articles/PMC8072628/ /pubmed/33923738 http://dx.doi.org/10.3390/biomedicines9040435 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Žnidarič, Matej Žurga, Žan Michel Maver, Uroš Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review |
title | Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review |
title_full | Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review |
title_fullStr | Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review |
title_full_unstemmed | Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review |
title_short | Design of In Vitro Hair Follicles for Different Applications in the Treatment of Alopecia—A Review |
title_sort | design of in vitro hair follicles for different applications in the treatment of alopecia—a review |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072628/ https://www.ncbi.nlm.nih.gov/pubmed/33923738 http://dx.doi.org/10.3390/biomedicines9040435 |
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