Cargando…
3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine
Recent advancements in tissue engineering and material science have radically improved in vitro culturing platforms to more accurately replicate human tissue. However, the transition to clinical relevance has been slow in part due to the lack of biologically compatible/relevant materials. In the pre...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072632/ https://www.ncbi.nlm.nih.gov/pubmed/33923751 http://dx.doi.org/10.3390/ma14082006 |
_version_ | 1783683951713320960 |
---|---|
author | Merk, Markus Chirikian, Orlando Adlhart, Christian |
author_facet | Merk, Markus Chirikian, Orlando Adlhart, Christian |
author_sort | Merk, Markus |
collection | PubMed |
description | Recent advancements in tissue engineering and material science have radically improved in vitro culturing platforms to more accurately replicate human tissue. However, the transition to clinical relevance has been slow in part due to the lack of biologically compatible/relevant materials. In the present study, we marry the commonly used two-dimensional (2D) technique of electrospinning and a self-assembly process to construct easily reproducible, highly porous, three-dimensional (3D) nanofiber scaffolds for various tissue engineering applications. Specimens from biologically relevant polymers polycaprolactone (PCL) and gelatin were chemically cross-linked using the naturally occurring cross-linker genipin. Potential cytotoxic effects of the scaffolds were analyzed by culturing human dermal fibroblasts (HDF) up to 23 days. The 3D PCL/gelatin/genipin scaffolds produced here resemble the complex nanofibrous architecture found in naturally occurring extracellular matrix (ECM) and exhibit physiologically relevant mechanical properties as well as excellent cell cytocompatibility. Samples cross-linked with 0.5% genipin demonstrated the highest metabolic activity and proliferation rates for HDF. Scanning electron microscopy (SEM) images indicated excellent cell adhesion and the characteristic morphological features of fibroblasts in all tested samples. The three-dimensional (3D) PCL/gelatin/genipin scaffolds produced here show great potential for various 3D tissue-engineering applications such as ex vivo cell culturing platforms, wound healing, or tissue replacement. |
format | Online Article Text |
id | pubmed-8072632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80726322021-04-27 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine Merk, Markus Chirikian, Orlando Adlhart, Christian Materials (Basel) Article Recent advancements in tissue engineering and material science have radically improved in vitro culturing platforms to more accurately replicate human tissue. However, the transition to clinical relevance has been slow in part due to the lack of biologically compatible/relevant materials. In the present study, we marry the commonly used two-dimensional (2D) technique of electrospinning and a self-assembly process to construct easily reproducible, highly porous, three-dimensional (3D) nanofiber scaffolds for various tissue engineering applications. Specimens from biologically relevant polymers polycaprolactone (PCL) and gelatin were chemically cross-linked using the naturally occurring cross-linker genipin. Potential cytotoxic effects of the scaffolds were analyzed by culturing human dermal fibroblasts (HDF) up to 23 days. The 3D PCL/gelatin/genipin scaffolds produced here resemble the complex nanofibrous architecture found in naturally occurring extracellular matrix (ECM) and exhibit physiologically relevant mechanical properties as well as excellent cell cytocompatibility. Samples cross-linked with 0.5% genipin demonstrated the highest metabolic activity and proliferation rates for HDF. Scanning electron microscopy (SEM) images indicated excellent cell adhesion and the characteristic morphological features of fibroblasts in all tested samples. The three-dimensional (3D) PCL/gelatin/genipin scaffolds produced here show great potential for various 3D tissue-engineering applications such as ex vivo cell culturing platforms, wound healing, or tissue replacement. MDPI 2021-04-16 /pmc/articles/PMC8072632/ /pubmed/33923751 http://dx.doi.org/10.3390/ma14082006 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Merk, Markus Chirikian, Orlando Adlhart, Christian 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine |
title | 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine |
title_full | 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine |
title_fullStr | 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine |
title_full_unstemmed | 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine |
title_short | 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine |
title_sort | 3d pcl/gelatin/genipin nanofiber sponge as scaffold for regenerative medicine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072632/ https://www.ncbi.nlm.nih.gov/pubmed/33923751 http://dx.doi.org/10.3390/ma14082006 |
work_keys_str_mv | AT merkmarkus 3dpclgelatingenipinnanofiberspongeasscaffoldforregenerativemedicine AT chirikianorlando 3dpclgelatingenipinnanofiberspongeasscaffoldforregenerativemedicine AT adlhartchristian 3dpclgelatingenipinnanofiberspongeasscaffoldforregenerativemedicine |