Cargando…
Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway
Obesity and hyperlipidemia are major risk factors for developing vascular diseases. Bee bread (BB) has been reported to exhibit some biological actions, including anti-obesity and anti-hyperlipidemic. This study aims to investigate whether bee bread can ameliorate vascular inflammation and impaired...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072722/ https://www.ncbi.nlm.nih.gov/pubmed/33921777 http://dx.doi.org/10.3390/ijms22084225 |
_version_ | 1783683972670160896 |
---|---|
author | Othman, Zaidatul Akmal Zakaria, Zaida Suleiman, Joseph Bagi Nna, Victor Udo Che Romli, Aminah Wan Ghazali, Wan Syaheedah Mohamed, Mahaneem |
author_facet | Othman, Zaidatul Akmal Zakaria, Zaida Suleiman, Joseph Bagi Nna, Victor Udo Che Romli, Aminah Wan Ghazali, Wan Syaheedah Mohamed, Mahaneem |
author_sort | Othman, Zaidatul Akmal |
collection | PubMed |
description | Obesity and hyperlipidemia are major risk factors for developing vascular diseases. Bee bread (BB) has been reported to exhibit some biological actions, including anti-obesity and anti-hyperlipidemic. This study aims to investigate whether bee bread can ameliorate vascular inflammation and impaired vasorelaxation activity through eNOS/NO/cGMP pathway in obese rats. Forty male Sprague-Dawley rats were randomly divided into four groups (n = 10/group), namely: control (normal group), obese rats (OB group), obese rats treated with bee bread (0.5 g/kg/day, OB/BB group) and obese rats treated with orlistat (10 mg/kg/day, OB/OR group). The latter three groups were given a high-fat diet (HFD) for 6 weeks to induced obesity before being administered with their respective treatments for another 6 weeks. After 12 weeks of the total experimental period, rats in the OB group demonstrated significantly higher Lee obesity index, lipid profile (total cholesterol, triglyceride, low-density lipoprotein), aortic proinflammatory markers (tumor necrosis factor-α, nuclear factor-κβ), aortic structural damage and impairment in vasorelaxation response to acetylcholine (ACh). Bee bread significantly ameliorated the obesity-induced vascular damage manifested by improvements in the lipid profile, aortic inflammatory markers, and the impaired vasorelaxation activity by significantly enhancing nitric oxide release, promoting endothelial nitric oxide synthase (eNOS) and cyclic guanosine monophosphate (cGMP) immunoexpression. These findings suggest that the administration of bee bread ameliorates the impaired vasorelaxation response to ACh by improving eNOS/NO/cGMP-signaling pathway in obese rats, suggesting its vascular therapeutic role. |
format | Online Article Text |
id | pubmed-8072722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80727222021-04-27 Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway Othman, Zaidatul Akmal Zakaria, Zaida Suleiman, Joseph Bagi Nna, Victor Udo Che Romli, Aminah Wan Ghazali, Wan Syaheedah Mohamed, Mahaneem Int J Mol Sci Article Obesity and hyperlipidemia are major risk factors for developing vascular diseases. Bee bread (BB) has been reported to exhibit some biological actions, including anti-obesity and anti-hyperlipidemic. This study aims to investigate whether bee bread can ameliorate vascular inflammation and impaired vasorelaxation activity through eNOS/NO/cGMP pathway in obese rats. Forty male Sprague-Dawley rats were randomly divided into four groups (n = 10/group), namely: control (normal group), obese rats (OB group), obese rats treated with bee bread (0.5 g/kg/day, OB/BB group) and obese rats treated with orlistat (10 mg/kg/day, OB/OR group). The latter three groups were given a high-fat diet (HFD) for 6 weeks to induced obesity before being administered with their respective treatments for another 6 weeks. After 12 weeks of the total experimental period, rats in the OB group demonstrated significantly higher Lee obesity index, lipid profile (total cholesterol, triglyceride, low-density lipoprotein), aortic proinflammatory markers (tumor necrosis factor-α, nuclear factor-κβ), aortic structural damage and impairment in vasorelaxation response to acetylcholine (ACh). Bee bread significantly ameliorated the obesity-induced vascular damage manifested by improvements in the lipid profile, aortic inflammatory markers, and the impaired vasorelaxation activity by significantly enhancing nitric oxide release, promoting endothelial nitric oxide synthase (eNOS) and cyclic guanosine monophosphate (cGMP) immunoexpression. These findings suggest that the administration of bee bread ameliorates the impaired vasorelaxation response to ACh by improving eNOS/NO/cGMP-signaling pathway in obese rats, suggesting its vascular therapeutic role. MDPI 2021-04-19 /pmc/articles/PMC8072722/ /pubmed/33921777 http://dx.doi.org/10.3390/ijms22084225 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Othman, Zaidatul Akmal Zakaria, Zaida Suleiman, Joseph Bagi Nna, Victor Udo Che Romli, Aminah Wan Ghazali, Wan Syaheedah Mohamed, Mahaneem Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway |
title | Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway |
title_full | Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway |
title_fullStr | Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway |
title_full_unstemmed | Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway |
title_short | Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling Pathway |
title_sort | bee bread ameliorates vascular inflammation and impaired vasorelaxation in obesity-induced vascular damage rat model: the role of enos/no/cgmp-signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072722/ https://www.ncbi.nlm.nih.gov/pubmed/33921777 http://dx.doi.org/10.3390/ijms22084225 |
work_keys_str_mv | AT othmanzaidatulakmal beebreadamelioratesvascularinflammationandimpairedvasorelaxationinobesityinducedvasculardamageratmodeltheroleofenosnocgmpsignalingpathway AT zakariazaida beebreadamelioratesvascularinflammationandimpairedvasorelaxationinobesityinducedvasculardamageratmodeltheroleofenosnocgmpsignalingpathway AT suleimanjosephbagi beebreadamelioratesvascularinflammationandimpairedvasorelaxationinobesityinducedvasculardamageratmodeltheroleofenosnocgmpsignalingpathway AT nnavictorudo beebreadamelioratesvascularinflammationandimpairedvasorelaxationinobesityinducedvasculardamageratmodeltheroleofenosnocgmpsignalingpathway AT cheromliaminah beebreadamelioratesvascularinflammationandimpairedvasorelaxationinobesityinducedvasculardamageratmodeltheroleofenosnocgmpsignalingpathway AT wanghazaliwansyaheedah beebreadamelioratesvascularinflammationandimpairedvasorelaxationinobesityinducedvasculardamageratmodeltheroleofenosnocgmpsignalingpathway AT mohamedmahaneem beebreadamelioratesvascularinflammationandimpairedvasorelaxationinobesityinducedvasculardamageratmodeltheroleofenosnocgmpsignalingpathway |