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Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries

Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied the farms....

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Autores principales: Nesporova, Kristina, Valcek, Adam, Papagiannitsis, Costas, Kutilova, Iva, Jamborova, Ivana, Davidova-Gerzova, Lenka, Bitar, Ibrahim, Hrabak, Jaroslav, Literak, Ivan, Dolejska, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072826/
https://www.ncbi.nlm.nih.gov/pubmed/33920558
http://dx.doi.org/10.3390/microorganisms9040866
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author Nesporova, Kristina
Valcek, Adam
Papagiannitsis, Costas
Kutilova, Iva
Jamborova, Ivana
Davidova-Gerzova, Lenka
Bitar, Ibrahim
Hrabak, Jaroslav
Literak, Ivan
Dolejska, Monika
author_facet Nesporova, Kristina
Valcek, Adam
Papagiannitsis, Costas
Kutilova, Iva
Jamborova, Ivana
Davidova-Gerzova, Lenka
Bitar, Ibrahim
Hrabak, Jaroslav
Literak, Ivan
Dolejska, Monika
author_sort Nesporova, Kristina
collection PubMed
description Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied the farms. A total of 62 Escherichia coli and 22 Klebsiella pneumoniae isolates were obtained and representative isolates were subjected to whole-genome sequencing. Relatively high prevalence of phylogenetic group D and F was observed in E. coli isolates and several zoonotic sequence types (STs) including ST457 (14 isolates), ST38 (5), ST10 (2), ST117 (2) or ST93 (4) were detected. Isolates from three farms, which purchased chicken from a Paraguayan hatchery showed higher prevalence of mcr-5.1 and bla(CTX-M-8) compared to the other nine farms, which purchased chickens from a Brazilian hatchery. Moreover, none of the K. pneumoniae isolates were linked to the Paraguayan hatchery. ESBL/AmpC and mcr-5-carrying multi-drug resistant (MDR) plasmids were characterized, and complete sequences were obtained for eight plasmids. The study shed light on Paraguayan poultry farms as a reservoir of antibiotic resistance commonly conferred via MDR plasmids and showed linkage between resistance and origin of the chickens at the hatcheries level.
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spelling pubmed-80728262021-04-27 Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries Nesporova, Kristina Valcek, Adam Papagiannitsis, Costas Kutilova, Iva Jamborova, Ivana Davidova-Gerzova, Lenka Bitar, Ibrahim Hrabak, Jaroslav Literak, Ivan Dolejska, Monika Microorganisms Article Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied the farms. A total of 62 Escherichia coli and 22 Klebsiella pneumoniae isolates were obtained and representative isolates were subjected to whole-genome sequencing. Relatively high prevalence of phylogenetic group D and F was observed in E. coli isolates and several zoonotic sequence types (STs) including ST457 (14 isolates), ST38 (5), ST10 (2), ST117 (2) or ST93 (4) were detected. Isolates from three farms, which purchased chicken from a Paraguayan hatchery showed higher prevalence of mcr-5.1 and bla(CTX-M-8) compared to the other nine farms, which purchased chickens from a Brazilian hatchery. Moreover, none of the K. pneumoniae isolates were linked to the Paraguayan hatchery. ESBL/AmpC and mcr-5-carrying multi-drug resistant (MDR) plasmids were characterized, and complete sequences were obtained for eight plasmids. The study shed light on Paraguayan poultry farms as a reservoir of antibiotic resistance commonly conferred via MDR plasmids and showed linkage between resistance and origin of the chickens at the hatcheries level. MDPI 2021-04-17 /pmc/articles/PMC8072826/ /pubmed/33920558 http://dx.doi.org/10.3390/microorganisms9040866 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nesporova, Kristina
Valcek, Adam
Papagiannitsis, Costas
Kutilova, Iva
Jamborova, Ivana
Davidova-Gerzova, Lenka
Bitar, Ibrahim
Hrabak, Jaroslav
Literak, Ivan
Dolejska, Monika
Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries
title Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries
title_full Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries
title_fullStr Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries
title_full_unstemmed Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries
title_short Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries
title_sort multi-drug resistant plasmids with esbl/ampc and mcr-5.1 in paraguayan poultry farms: the linkage of antibiotic resistance and hatcheries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072826/
https://www.ncbi.nlm.nih.gov/pubmed/33920558
http://dx.doi.org/10.3390/microorganisms9040866
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