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Hydroxyapatite-Coated SPIONs and Their Influence on Cytokine Release

Hydroxyapatite- or calcium phosphate-coated iron oxide nanoparticles have a high potential for use in many biomedical applications. In this study, a co-precipitation method for the synthesis of hydroxyapatite-coated nanoparticles (SPION(HAp)), was used. The produced nanoparticles have been character...

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Detalles Bibliográficos
Autores principales: Friedrich, Bernhard, Auger, Jean-Philippe, Dutz, Silvio, Cicha, Iwona, Schreiber, Eveline, Band, Julia, Boccacccini, Aldo R., Krönke, Gerhard, Alexiou, Christoph, Tietze, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072956/
https://www.ncbi.nlm.nih.gov/pubmed/33923700
http://dx.doi.org/10.3390/ijms22084143
Descripción
Sumario:Hydroxyapatite- or calcium phosphate-coated iron oxide nanoparticles have a high potential for use in many biomedical applications. In this study, a co-precipitation method for the synthesis of hydroxyapatite-coated nanoparticles (SPION(HAp)), was used. The produced nanoparticles have been characterized by dynamic light scattering, X-ray diffraction, vibrating sample magnetometry, Fourier transform infrared spectrometry, atomic emission spectroscopy, scanning electron microscopy, transmission electron microscopy, selected area diffraction, and energy-dispersive X-ray spectroscopy. The results showed a successful synthesis of 190 nm sized particles and their stable coating, resulting in SPION(HAp). Potential cytotoxic effects of SPION(HAp) on EL4, THP-1, and Jurkat cells were tested, showing only a minor effect on cell viability at the highest tested concentration (400 µg Fe/mL). The results further showed that hydroxyapatite-coated SPIONs can induce minor TNF-α and IL-6 release by murine macrophages at a concentration of 100 µg Fe/mL. To investigate if and how such particles interact with other substances that modulate the immune response, SPION(HAp)-treated macrophages were incubated with LPS (lipopolysaccharides) and dexamethasone. We found that cytokine release in response to these potent pro- and anti-inflammatory agents was modulated in the presence of SPION(HAp). Knowledge of this behavior is important for the management of inflammatory processes following in vivo applications of this type of SPIONs.