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Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)

Impaired fetal growth is one of the most important causes of prematurity, stillbirth and infant mortality. The pathogenesis of idiopathic fetal growth restriction (FGR) is poorly understood but is thought to be multifactorial and comprise a range of genetic causes. This research aimed to investigate...

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Autores principales: Lipka, Aleksandra, Jastrzebski, Jan Pawel, Paukszto, Lukasz, Makowczenko, Karol Gustaw, Lopienska-Biernat, Elzbieta, Gowkielewicz, Marek, Lepiarczyk, Ewa, Wiszpolska, Marta, Majewski, Mariusz Krzysztof, Majewska, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072961/
https://www.ncbi.nlm.nih.gov/pubmed/33923632
http://dx.doi.org/10.3390/cells10040921
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author Lipka, Aleksandra
Jastrzebski, Jan Pawel
Paukszto, Lukasz
Makowczenko, Karol Gustaw
Lopienska-Biernat, Elzbieta
Gowkielewicz, Marek
Lepiarczyk, Ewa
Wiszpolska, Marta
Majewski, Mariusz Krzysztof
Majewska, Marta
author_facet Lipka, Aleksandra
Jastrzebski, Jan Pawel
Paukszto, Lukasz
Makowczenko, Karol Gustaw
Lopienska-Biernat, Elzbieta
Gowkielewicz, Marek
Lepiarczyk, Ewa
Wiszpolska, Marta
Majewski, Mariusz Krzysztof
Majewska, Marta
author_sort Lipka, Aleksandra
collection PubMed
description Impaired fetal growth is one of the most important causes of prematurity, stillbirth and infant mortality. The pathogenesis of idiopathic fetal growth restriction (FGR) is poorly understood but is thought to be multifactorial and comprise a range of genetic causes. This research aimed to investigate non-coding RNAs (lncRNAs) in the placentas of male and female fetuses affected by FGR. RNA-Seq data were analyzed to detect lncRNAs, their potential target genes and circular RNAs (circRNAs); a differential analysis was also performed. The multilevel bioinformatic analysis enabled the detection of 23,137 placental lncRNAs and 4263 of them were classified as novel. In FGR-affected female fetuses’ placentas (ff-FGR), among 19 transcriptionally active regions (TARs), five differentially expressed lncRNAs (DELs) and 12 differentially expressed protein-coding genes (DEGs) were identified. Within 232 differentially expressed TARs identified in male fetuses (mf-FGR), 33 encompassed novel and 176 known lncRNAs, and 52 DEGs were upregulated, while 180 revealed decreased expression. In ff-FGR ACTA2-AS1, lncRNA expression was significantly correlated with five DEGs, and in mf-FGR, 25 TARs were associated with DELs correlated with 157 unique DEGs. Backsplicing circRNA processes were detected in the range of H19 lncRNA, in both ff- and mf-FGR placentas. The performed global lncRNAs characteristics in terms of fetal sex showed dysregulation of DELs, DEGs and circRNAs that may affect fetus growth and pregnancy outcomes. In female placentas, DELs and DEGs were associated mainly with the vasculature, while in male placentas, disturbed expression predominantly affected immune processes.
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spelling pubmed-80729612021-04-27 Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR) Lipka, Aleksandra Jastrzebski, Jan Pawel Paukszto, Lukasz Makowczenko, Karol Gustaw Lopienska-Biernat, Elzbieta Gowkielewicz, Marek Lepiarczyk, Ewa Wiszpolska, Marta Majewski, Mariusz Krzysztof Majewska, Marta Cells Article Impaired fetal growth is one of the most important causes of prematurity, stillbirth and infant mortality. The pathogenesis of idiopathic fetal growth restriction (FGR) is poorly understood but is thought to be multifactorial and comprise a range of genetic causes. This research aimed to investigate non-coding RNAs (lncRNAs) in the placentas of male and female fetuses affected by FGR. RNA-Seq data were analyzed to detect lncRNAs, their potential target genes and circular RNAs (circRNAs); a differential analysis was also performed. The multilevel bioinformatic analysis enabled the detection of 23,137 placental lncRNAs and 4263 of them were classified as novel. In FGR-affected female fetuses’ placentas (ff-FGR), among 19 transcriptionally active regions (TARs), five differentially expressed lncRNAs (DELs) and 12 differentially expressed protein-coding genes (DEGs) were identified. Within 232 differentially expressed TARs identified in male fetuses (mf-FGR), 33 encompassed novel and 176 known lncRNAs, and 52 DEGs were upregulated, while 180 revealed decreased expression. In ff-FGR ACTA2-AS1, lncRNA expression was significantly correlated with five DEGs, and in mf-FGR, 25 TARs were associated with DELs correlated with 157 unique DEGs. Backsplicing circRNA processes were detected in the range of H19 lncRNA, in both ff- and mf-FGR placentas. The performed global lncRNAs characteristics in terms of fetal sex showed dysregulation of DELs, DEGs and circRNAs that may affect fetus growth and pregnancy outcomes. In female placentas, DELs and DEGs were associated mainly with the vasculature, while in male placentas, disturbed expression predominantly affected immune processes. MDPI 2021-04-16 /pmc/articles/PMC8072961/ /pubmed/33923632 http://dx.doi.org/10.3390/cells10040921 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lipka, Aleksandra
Jastrzebski, Jan Pawel
Paukszto, Lukasz
Makowczenko, Karol Gustaw
Lopienska-Biernat, Elzbieta
Gowkielewicz, Marek
Lepiarczyk, Ewa
Wiszpolska, Marta
Majewski, Mariusz Krzysztof
Majewska, Marta
Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)
title Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)
title_full Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)
title_fullStr Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)
title_full_unstemmed Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)
title_short Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)
title_sort sex-biased lncrna signature in fetal growth restriction (fgr)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072961/
https://www.ncbi.nlm.nih.gov/pubmed/33923632
http://dx.doi.org/10.3390/cells10040921
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