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Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents

Stem cell therapy is one of the most promising candidate treatments for spinal cord injury. Research has shown optimistic results for this therapy, but clinical limitations remain, including poor viability, engraftment, and differentiation. Here, we isolated novel peripheral nerve-derived stem cells...

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Autores principales: Lee, Hye-Lan, Yeum, Chung-Eun, Lee, HyeYeong, Oh, Jinsoo, Kim, Jong-Tae, Lee, Won-Jin, Ha, Yoon, Yang, Young-Il, Kim, Keung-Nyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072978/
https://www.ncbi.nlm.nih.gov/pubmed/33923671
http://dx.doi.org/10.3390/ijms22084141
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author Lee, Hye-Lan
Yeum, Chung-Eun
Lee, HyeYeong
Oh, Jinsoo
Kim, Jong-Tae
Lee, Won-Jin
Ha, Yoon
Yang, Young-Il
Kim, Keung-Nyun
author_facet Lee, Hye-Lan
Yeum, Chung-Eun
Lee, HyeYeong
Oh, Jinsoo
Kim, Jong-Tae
Lee, Won-Jin
Ha, Yoon
Yang, Young-Il
Kim, Keung-Nyun
author_sort Lee, Hye-Lan
collection PubMed
description Stem cell therapy is one of the most promising candidate treatments for spinal cord injury. Research has shown optimistic results for this therapy, but clinical limitations remain, including poor viability, engraftment, and differentiation. Here, we isolated novel peripheral nerve-derived stem cells (PNSCs) from adult peripheral nerves with similar characteristics to neural-crest stem cells. These PNSCs expressed neural-crest specific markers and showed multilineage differentiation potential into Schwann cells, neuroglia, neurons, and mesodermal cells. In addition, PNSCs showed therapeutic potential by releasing the neurotrophic factors, including glial cell-line-derived neurotrophic factor, insulin-like growth factor, nerve growth factor, and neurotrophin-3. PNSC abilities were also enhanced by their development into spheroids which secreted neurotrophic factors several times more than non-spheroid PNSCs and expressed several types of extra cellular matrix. These features suggest that the potential for these PNSC spheroids can overcome their limitations. In an animal spinal cord injury (SCI) model, these PNSC spheroids induced functional recovery and neuronal regeneration. These PNSC spheroids also reduced the neuropathic pain which accompanies SCI after remyelination. These PNSC spheroids may represent a new therapeutic approach for patients suffering from SCI.
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spelling pubmed-80729782021-04-27 Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents Lee, Hye-Lan Yeum, Chung-Eun Lee, HyeYeong Oh, Jinsoo Kim, Jong-Tae Lee, Won-Jin Ha, Yoon Yang, Young-Il Kim, Keung-Nyun Int J Mol Sci Article Stem cell therapy is one of the most promising candidate treatments for spinal cord injury. Research has shown optimistic results for this therapy, but clinical limitations remain, including poor viability, engraftment, and differentiation. Here, we isolated novel peripheral nerve-derived stem cells (PNSCs) from adult peripheral nerves with similar characteristics to neural-crest stem cells. These PNSCs expressed neural-crest specific markers and showed multilineage differentiation potential into Schwann cells, neuroglia, neurons, and mesodermal cells. In addition, PNSCs showed therapeutic potential by releasing the neurotrophic factors, including glial cell-line-derived neurotrophic factor, insulin-like growth factor, nerve growth factor, and neurotrophin-3. PNSC abilities were also enhanced by their development into spheroids which secreted neurotrophic factors several times more than non-spheroid PNSCs and expressed several types of extra cellular matrix. These features suggest that the potential for these PNSC spheroids can overcome their limitations. In an animal spinal cord injury (SCI) model, these PNSC spheroids induced functional recovery and neuronal regeneration. These PNSC spheroids also reduced the neuropathic pain which accompanies SCI after remyelination. These PNSC spheroids may represent a new therapeutic approach for patients suffering from SCI. MDPI 2021-04-16 /pmc/articles/PMC8072978/ /pubmed/33923671 http://dx.doi.org/10.3390/ijms22084141 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Hye-Lan
Yeum, Chung-Eun
Lee, HyeYeong
Oh, Jinsoo
Kim, Jong-Tae
Lee, Won-Jin
Ha, Yoon
Yang, Young-Il
Kim, Keung-Nyun
Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
title Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
title_full Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
title_fullStr Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
title_full_unstemmed Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
title_short Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
title_sort peripheral nerve-derived stem cell spheroids induce functional recovery and repair after spinal cord injury in rodents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072978/
https://www.ncbi.nlm.nih.gov/pubmed/33923671
http://dx.doi.org/10.3390/ijms22084141
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