Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma
Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as microbe...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073006/ https://www.ncbi.nlm.nih.gov/pubmed/33921693 http://dx.doi.org/10.3390/ijms22084214 |
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author | Anand, Gautam Leibman-Markus, Meirav Elkabetz, Dorin Bar, Maya |
author_facet | Anand, Gautam Leibman-Markus, Meirav Elkabetz, Dorin Bar, Maya |
author_sort | Anand, Gautam |
collection | PubMed |
description | Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as microbe-associated molecular patterns (MAMPs). These components are recognized by pattern recognition receptors (PRRs) which include plant cell surface receptors. Upon recognition, PRRs trigger pattern-triggered immunity (PTI). Ethylene Inducing Xylanase (EIX) is a fungal MAMP protein from the plant-growth-promoting fungi (PGPF)–Trichoderma. It elicits plant defense responses in tobacco (Nicotiana tabacum) and tomato (Solanum lycopersicum), making it an excellent tool in the studies of plant immunity. Xylanases such as EIX are hydrolytic enzymes that act on xylan in hemicellulose. There are two types of xylanases: the endo-1, 4-β-xylanases that hydrolyze within the xylan structure, and the β-d-xylosidases that hydrolyze the ends of the xylan chain. Xylanases are mainly synthesized by fungi and bacteria. Filamentous fungi produce xylanases in high amounts and secrete them in liquid cultures, making them an ideal system for xylanase purification. Here, we describe a method for cost- and yield-effective xylanase production from Trichoderma using wheat bran as a growth substrate. Xylanase produced by this method possessed xylanase activity and immunogenic activity, effectively inducing a hypersensitive response, ethylene biosynthesis, and ROS burst. |
format | Online Article Text |
id | pubmed-8073006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80730062021-04-27 Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma Anand, Gautam Leibman-Markus, Meirav Elkabetz, Dorin Bar, Maya Int J Mol Sci Article Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as microbe-associated molecular patterns (MAMPs). These components are recognized by pattern recognition receptors (PRRs) which include plant cell surface receptors. Upon recognition, PRRs trigger pattern-triggered immunity (PTI). Ethylene Inducing Xylanase (EIX) is a fungal MAMP protein from the plant-growth-promoting fungi (PGPF)–Trichoderma. It elicits plant defense responses in tobacco (Nicotiana tabacum) and tomato (Solanum lycopersicum), making it an excellent tool in the studies of plant immunity. Xylanases such as EIX are hydrolytic enzymes that act on xylan in hemicellulose. There are two types of xylanases: the endo-1, 4-β-xylanases that hydrolyze within the xylan structure, and the β-d-xylosidases that hydrolyze the ends of the xylan chain. Xylanases are mainly synthesized by fungi and bacteria. Filamentous fungi produce xylanases in high amounts and secrete them in liquid cultures, making them an ideal system for xylanase purification. Here, we describe a method for cost- and yield-effective xylanase production from Trichoderma using wheat bran as a growth substrate. Xylanase produced by this method possessed xylanase activity and immunogenic activity, effectively inducing a hypersensitive response, ethylene biosynthesis, and ROS burst. MDPI 2021-04-19 /pmc/articles/PMC8073006/ /pubmed/33921693 http://dx.doi.org/10.3390/ijms22084214 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Anand, Gautam Leibman-Markus, Meirav Elkabetz, Dorin Bar, Maya Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma |
title | Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma |
title_full | Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma |
title_fullStr | Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma |
title_full_unstemmed | Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma |
title_short | Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma |
title_sort | method for the production and purification of plant immuno-active xylanase from trichoderma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073006/ https://www.ncbi.nlm.nih.gov/pubmed/33921693 http://dx.doi.org/10.3390/ijms22084214 |
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