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The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells
Parvovirus B19 (B19V) is a widespread human pathogen possessing a high tropism for erythroid precursor cells. However, the persistence or active replication of B19V in endothelial cells (EC) has been detected in diverse human pathologies. The VP1 unique region (VP1u) of the viral capsid has been rep...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073096/ https://www.ncbi.nlm.nih.gov/pubmed/33921883 http://dx.doi.org/10.3390/biom11040606 |
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author | Rinkūnaitė, Ieva Šimoliūnas, Egidijus Bironaitė, Daiva Rutkienė, Rasa Bukelskienė, Virginija Meškys, Rolandas Bogomolovas, Julius |
author_facet | Rinkūnaitė, Ieva Šimoliūnas, Egidijus Bironaitė, Daiva Rutkienė, Rasa Bukelskienė, Virginija Meškys, Rolandas Bogomolovas, Julius |
author_sort | Rinkūnaitė, Ieva |
collection | PubMed |
description | Parvovirus B19 (B19V) is a widespread human pathogen possessing a high tropism for erythroid precursor cells. However, the persistence or active replication of B19V in endothelial cells (EC) has been detected in diverse human pathologies. The VP1 unique region (VP1u) of the viral capsid has been reported to act as a major determinant of viral tropism for erythroid precursor cells. Nevertheless, the interaction of VP1u with EC has not been studied. We demonstrate that recombinant VP1u is efficiently internalized by rats’ pulmonary trunk blood vessel-derived EC in vitro compared to the human umbilical vein EC line. The exposure to VP1u was not acutely cytotoxic to either human- or rat-derived ECs, but led to the upregulation of cellular stress signaling-related pathways. Our data suggest that high levels of circulating B19V during acute infection can cause endothelial damage, even without active replication or direct internalization into the cells. |
format | Online Article Text |
id | pubmed-8073096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80730962021-04-27 The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells Rinkūnaitė, Ieva Šimoliūnas, Egidijus Bironaitė, Daiva Rutkienė, Rasa Bukelskienė, Virginija Meškys, Rolandas Bogomolovas, Julius Biomolecules Article Parvovirus B19 (B19V) is a widespread human pathogen possessing a high tropism for erythroid precursor cells. However, the persistence or active replication of B19V in endothelial cells (EC) has been detected in diverse human pathologies. The VP1 unique region (VP1u) of the viral capsid has been reported to act as a major determinant of viral tropism for erythroid precursor cells. Nevertheless, the interaction of VP1u with EC has not been studied. We demonstrate that recombinant VP1u is efficiently internalized by rats’ pulmonary trunk blood vessel-derived EC in vitro compared to the human umbilical vein EC line. The exposure to VP1u was not acutely cytotoxic to either human- or rat-derived ECs, but led to the upregulation of cellular stress signaling-related pathways. Our data suggest that high levels of circulating B19V during acute infection can cause endothelial damage, even without active replication or direct internalization into the cells. MDPI 2021-04-19 /pmc/articles/PMC8073096/ /pubmed/33921883 http://dx.doi.org/10.3390/biom11040606 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rinkūnaitė, Ieva Šimoliūnas, Egidijus Bironaitė, Daiva Rutkienė, Rasa Bukelskienė, Virginija Meškys, Rolandas Bogomolovas, Julius The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells |
title | The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells |
title_full | The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells |
title_fullStr | The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells |
title_full_unstemmed | The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells |
title_short | The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells |
title_sort | effect of a unique region of parvovirus b19 capsid protein vp1 on endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073096/ https://www.ncbi.nlm.nih.gov/pubmed/33921883 http://dx.doi.org/10.3390/biom11040606 |
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