Cargando…

Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs

Placental insufficiency is a known consequence of maternal heat stress during gestation in farm animals. The molecular regulation of placentae during the stress response is little known in pigs. This study aims to identify differential gene expression in pig placentae caused by maternal heat exposur...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Weicheng, Liu, Fan, Marth, Christina D., Green, Mark P., Le, Hieu H., Leury, Brian J., Bell, Alan W., Dunshea, Frank R., Cottrell, Jeremy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073098/
https://www.ncbi.nlm.nih.gov/pubmed/33923747
http://dx.doi.org/10.3390/ijms22084147
_version_ 1783684055569530880
author Zhao, Weicheng
Liu, Fan
Marth, Christina D.
Green, Mark P.
Le, Hieu H.
Leury, Brian J.
Bell, Alan W.
Dunshea, Frank R.
Cottrell, Jeremy J.
author_facet Zhao, Weicheng
Liu, Fan
Marth, Christina D.
Green, Mark P.
Le, Hieu H.
Leury, Brian J.
Bell, Alan W.
Dunshea, Frank R.
Cottrell, Jeremy J.
author_sort Zhao, Weicheng
collection PubMed
description Placental insufficiency is a known consequence of maternal heat stress during gestation in farm animals. The molecular regulation of placentae during the stress response is little known in pigs. This study aims to identify differential gene expression in pig placentae caused by maternal heat exposure during early to mid-gestation. RNA sequencing (RNA-seq) was performed on female placental samples from pregnant pigs exposed to thermoneutral control (CON; constant 20 °C; n = 5) or cyclic heat stress (HS; cyclic 28 to 33 °C; n = 5) conditions between d40 and d60 of gestation. On d60 of gestation, placental efficiency (fetal/placental weight) was decreased (p = 0.023) by maternal HS. A total of 169 genes were differentially expressed (FDR ≤ 0.1) between CON and HS placentae of female fetuses, of which 35 genes were upregulated and 134 genes were downregulated by maternal HS. The current data revealed transport activity (FDR = 0.027), glycoprotein biosynthetic process (FDR = 0.044), and carbohydrate metabolic process (FDR = 0.049) among the terms enriched by the downregulated genes (HS vs. CON). In addition, solute carrier (SLC)-mediated transmembrane transport (FDR = 0.008) and glycosaminoglycan biosynthesis (FDR = 0.027), which modulates placental stroma synthesis, were identified among the pathways enriched by the downregulated genes. These findings provide evidence that heat-stress induced placental inefficiency may be underpinned by altered expression of genes associated with placental nutrient transport capacity and metabolism. A further understanding of the molecular mechanism contributes to the identification of placental gene signatures of summer infertility in pigs.
format Online
Article
Text
id pubmed-8073098
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80730982021-04-27 Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs Zhao, Weicheng Liu, Fan Marth, Christina D. Green, Mark P. Le, Hieu H. Leury, Brian J. Bell, Alan W. Dunshea, Frank R. Cottrell, Jeremy J. Int J Mol Sci Article Placental insufficiency is a known consequence of maternal heat stress during gestation in farm animals. The molecular regulation of placentae during the stress response is little known in pigs. This study aims to identify differential gene expression in pig placentae caused by maternal heat exposure during early to mid-gestation. RNA sequencing (RNA-seq) was performed on female placental samples from pregnant pigs exposed to thermoneutral control (CON; constant 20 °C; n = 5) or cyclic heat stress (HS; cyclic 28 to 33 °C; n = 5) conditions between d40 and d60 of gestation. On d60 of gestation, placental efficiency (fetal/placental weight) was decreased (p = 0.023) by maternal HS. A total of 169 genes were differentially expressed (FDR ≤ 0.1) between CON and HS placentae of female fetuses, of which 35 genes were upregulated and 134 genes were downregulated by maternal HS. The current data revealed transport activity (FDR = 0.027), glycoprotein biosynthetic process (FDR = 0.044), and carbohydrate metabolic process (FDR = 0.049) among the terms enriched by the downregulated genes (HS vs. CON). In addition, solute carrier (SLC)-mediated transmembrane transport (FDR = 0.008) and glycosaminoglycan biosynthesis (FDR = 0.027), which modulates placental stroma synthesis, were identified among the pathways enriched by the downregulated genes. These findings provide evidence that heat-stress induced placental inefficiency may be underpinned by altered expression of genes associated with placental nutrient transport capacity and metabolism. A further understanding of the molecular mechanism contributes to the identification of placental gene signatures of summer infertility in pigs. MDPI 2021-04-16 /pmc/articles/PMC8073098/ /pubmed/33923747 http://dx.doi.org/10.3390/ijms22084147 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Weicheng
Liu, Fan
Marth, Christina D.
Green, Mark P.
Le, Hieu H.
Leury, Brian J.
Bell, Alan W.
Dunshea, Frank R.
Cottrell, Jeremy J.
Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs
title Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs
title_full Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs
title_fullStr Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs
title_full_unstemmed Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs
title_short Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs
title_sort maternal heat stress alters expression of genes associated with nutrient transport activity and metabolism in female placentae from mid-gestating pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073098/
https://www.ncbi.nlm.nih.gov/pubmed/33923747
http://dx.doi.org/10.3390/ijms22084147
work_keys_str_mv AT zhaoweicheng maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT liufan maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT marthchristinad maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT greenmarkp maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT lehieuh maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT leurybrianj maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT bellalanw maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT dunsheafrankr maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs
AT cottrelljeremyj maternalheatstressaltersexpressionofgenesassociatedwithnutrienttransportactivityandmetabolisminfemaleplacentaefrommidgestatingpigs