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Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans

The ductus arteriosus (DA) immediately starts closing after birth. This dynamic process involves DA-specific properties, including highly differentiated smooth muscle, sparse elastic fibers, and intimal thickening (IT). Although several studies have demonstrated DA-specific gene expressions using an...

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Autores principales: Saito, Junichi, Kojima, Tomoyuki, Tanifuji, Shota, Kato, Yuko, Oka, Sayuki, Ichikawa, Yasuhiro, Miyagi, Etsuko, Tachibana, Tsuyoshi, Asou, Toshihide, Yokoyama, Utako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073410/
https://www.ncbi.nlm.nih.gov/pubmed/33923468
http://dx.doi.org/10.3390/jcdd8040045
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author Saito, Junichi
Kojima, Tomoyuki
Tanifuji, Shota
Kato, Yuko
Oka, Sayuki
Ichikawa, Yasuhiro
Miyagi, Etsuko
Tachibana, Tsuyoshi
Asou, Toshihide
Yokoyama, Utako
author_facet Saito, Junichi
Kojima, Tomoyuki
Tanifuji, Shota
Kato, Yuko
Oka, Sayuki
Ichikawa, Yasuhiro
Miyagi, Etsuko
Tachibana, Tsuyoshi
Asou, Toshihide
Yokoyama, Utako
author_sort Saito, Junichi
collection PubMed
description The ductus arteriosus (DA) immediately starts closing after birth. This dynamic process involves DA-specific properties, including highly differentiated smooth muscle, sparse elastic fibers, and intimal thickening (IT). Although several studies have demonstrated DA-specific gene expressions using animal tissues and human fetuses, the transcriptional profiles of the closing DA and the patent DA remain largely unknown. We performed transcriptome analysis using four human DA samples. The three closing DA samples exhibited typical DA morphology, but the patent DA exhibited aorta-like elastic lamellae and poorly formed IT. A cluster analysis revealed that samples were clearly divided into two major clusters, the closing DA and patent DA clusters, and showed distinct gene expression profiles in IT and the tunica media of the closing DA samples. Cardiac neural crest-related genes such as JAG1 were highly expressed in the tunica media and IT of the closing DA samples compared to the patent DA sample. Abundant protein expressions of jagged 1 and the differentiated smooth muscle marker calponin were observed in the closing DA samples but not in the patent DA sample. Second heart field-related genes such as ISL1 were enriched in the patent DA sample. These data indicate that the patent DA may have different cell lineages compared to the closing DA.
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spelling pubmed-80734102021-04-27 Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans Saito, Junichi Kojima, Tomoyuki Tanifuji, Shota Kato, Yuko Oka, Sayuki Ichikawa, Yasuhiro Miyagi, Etsuko Tachibana, Tsuyoshi Asou, Toshihide Yokoyama, Utako J Cardiovasc Dev Dis Article The ductus arteriosus (DA) immediately starts closing after birth. This dynamic process involves DA-specific properties, including highly differentiated smooth muscle, sparse elastic fibers, and intimal thickening (IT). Although several studies have demonstrated DA-specific gene expressions using animal tissues and human fetuses, the transcriptional profiles of the closing DA and the patent DA remain largely unknown. We performed transcriptome analysis using four human DA samples. The three closing DA samples exhibited typical DA morphology, but the patent DA exhibited aorta-like elastic lamellae and poorly formed IT. A cluster analysis revealed that samples were clearly divided into two major clusters, the closing DA and patent DA clusters, and showed distinct gene expression profiles in IT and the tunica media of the closing DA samples. Cardiac neural crest-related genes such as JAG1 were highly expressed in the tunica media and IT of the closing DA samples compared to the patent DA sample. Abundant protein expressions of jagged 1 and the differentiated smooth muscle marker calponin were observed in the closing DA samples but not in the patent DA sample. Second heart field-related genes such as ISL1 were enriched in the patent DA sample. These data indicate that the patent DA may have different cell lineages compared to the closing DA. MDPI 2021-04-16 /pmc/articles/PMC8073410/ /pubmed/33923468 http://dx.doi.org/10.3390/jcdd8040045 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saito, Junichi
Kojima, Tomoyuki
Tanifuji, Shota
Kato, Yuko
Oka, Sayuki
Ichikawa, Yasuhiro
Miyagi, Etsuko
Tachibana, Tsuyoshi
Asou, Toshihide
Yokoyama, Utako
Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans
title Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans
title_full Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans
title_fullStr Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans
title_full_unstemmed Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans
title_short Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans
title_sort transcriptome analysis reveals differential gene expression between the closing ductus arteriosus and the patent ductus arteriosus in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073410/
https://www.ncbi.nlm.nih.gov/pubmed/33923468
http://dx.doi.org/10.3390/jcdd8040045
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