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Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells

Recent research has indicated that dysbiosis of the gut microbiota can lead to an altered circadian clock of the mammalian host. Herein we developed an original system that allows real-time circadian studies of human HepG2 hepatoma cells co-cultured with bacteria. The HepG2 cells with stably integra...

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Autores principales: Kovač, Urša, Žužek, Zala, Raspor Dall’Olio, Lucija, Pohar, Katka, Ihan, Alojz, Moškon, Miha, Rozman, Damjana, Starčič Erjavec, Marjanca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073551/
https://www.ncbi.nlm.nih.gov/pubmed/33920679
http://dx.doi.org/10.3390/microorganisms9040869
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author Kovač, Urša
Žužek, Zala
Raspor Dall’Olio, Lucija
Pohar, Katka
Ihan, Alojz
Moškon, Miha
Rozman, Damjana
Starčič Erjavec, Marjanca
author_facet Kovač, Urša
Žužek, Zala
Raspor Dall’Olio, Lucija
Pohar, Katka
Ihan, Alojz
Moškon, Miha
Rozman, Damjana
Starčič Erjavec, Marjanca
author_sort Kovač, Urša
collection PubMed
description Recent research has indicated that dysbiosis of the gut microbiota can lead to an altered circadian clock of the mammalian host. Herein we developed an original system that allows real-time circadian studies of human HepG2 hepatoma cells co-cultured with bacteria. The HepG2 cells with stably integrated firefly luciferase reporter under the control of PERIOD2 promoter were co-cultured with E. coli strains isolated from human fecal samples from healthy individuals. The two E. coli strains differ in the phylogenetic group and the number of ExPEC virulence-associated genes: BJ17 has only two, and BJ23 has 15 of 23 tested. In the first 24 h, the E. coli BJ17 affected the HepG2 circadian clock more than BJ23. Cosinor analysis shows a statistically significant change in the amplitude of PER1 and 2 and the phase advance of PER3. A high percentage of necrotic and apoptotic cells occurred at 72 h, while a correlation between the number of ExPEC genes and the influence on the HepG2 core clock gene expression was observed. Our study reveals that the E. coli genetic background is important for the effect on the mammalian circadian clock genes, indicating possible future use of probiotic E. coli strains to influence the host circadian clock.
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spelling pubmed-80735512021-04-27 Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells Kovač, Urša Žužek, Zala Raspor Dall’Olio, Lucija Pohar, Katka Ihan, Alojz Moškon, Miha Rozman, Damjana Starčič Erjavec, Marjanca Microorganisms Communication Recent research has indicated that dysbiosis of the gut microbiota can lead to an altered circadian clock of the mammalian host. Herein we developed an original system that allows real-time circadian studies of human HepG2 hepatoma cells co-cultured with bacteria. The HepG2 cells with stably integrated firefly luciferase reporter under the control of PERIOD2 promoter were co-cultured with E. coli strains isolated from human fecal samples from healthy individuals. The two E. coli strains differ in the phylogenetic group and the number of ExPEC virulence-associated genes: BJ17 has only two, and BJ23 has 15 of 23 tested. In the first 24 h, the E. coli BJ17 affected the HepG2 circadian clock more than BJ23. Cosinor analysis shows a statistically significant change in the amplitude of PER1 and 2 and the phase advance of PER3. A high percentage of necrotic and apoptotic cells occurred at 72 h, while a correlation between the number of ExPEC genes and the influence on the HepG2 core clock gene expression was observed. Our study reveals that the E. coli genetic background is important for the effect on the mammalian circadian clock genes, indicating possible future use of probiotic E. coli strains to influence the host circadian clock. MDPI 2021-04-17 /pmc/articles/PMC8073551/ /pubmed/33920679 http://dx.doi.org/10.3390/microorganisms9040869 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kovač, Urša
Žužek, Zala
Raspor Dall’Olio, Lucija
Pohar, Katka
Ihan, Alojz
Moškon, Miha
Rozman, Damjana
Starčič Erjavec, Marjanca
Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells
title Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells
title_full Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells
title_fullStr Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells
title_full_unstemmed Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells
title_short Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells
title_sort escherichia coli affects expression of circadian clock genes in human hepatoma cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073551/
https://www.ncbi.nlm.nih.gov/pubmed/33920679
http://dx.doi.org/10.3390/microorganisms9040869
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