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Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line
In Glioblastoma Multiforme (GBM), hypoxia is associated with radioresistance and poor prognosis. Since standard GBM treatments are not always effective, new strategies are needed to overcome resistance to therapeutic treatments, including radiotherapy (RT). Our study aims to shed light on the biomar...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073933/ https://www.ncbi.nlm.nih.gov/pubmed/33923454 http://dx.doi.org/10.3390/jpm11040308 |
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author | Bravatà, Valentina Tinganelli, Walter Cammarata, Francesco P. Minafra, Luigi Calvaruso, Marco Sokol, Olga Petringa, Giada Cirrone, Giuseppe A.P. Scifoni, Emanuele Forte, Giusi I. Russo, Giorgio |
author_facet | Bravatà, Valentina Tinganelli, Walter Cammarata, Francesco P. Minafra, Luigi Calvaruso, Marco Sokol, Olga Petringa, Giada Cirrone, Giuseppe A.P. Scifoni, Emanuele Forte, Giusi I. Russo, Giorgio |
author_sort | Bravatà, Valentina |
collection | PubMed |
description | In Glioblastoma Multiforme (GBM), hypoxia is associated with radioresistance and poor prognosis. Since standard GBM treatments are not always effective, new strategies are needed to overcome resistance to therapeutic treatments, including radiotherapy (RT). Our study aims to shed light on the biomarker network involved in a hypoxic (0.2% oxygen) GBM cell line that is radioresistant after proton therapy (PT). For cultivating cells in acute hypoxia, GSI’s hypoxic chambers were used. Cells were irradiated in the middle of a spread-out Bragg peak with increasing PT doses to verify the greater radioresistance in hypoxic conditions. Whole-genome cDNA microarray gene expression analyses were performed for samples treated with 2 and 10 Gy to highlight biological processes activated in GBM following PT in the hypoxic condition. We describe cell survival response and significant deregulated pathways responsible for the cell death/survival balance and gene signatures linked to the PT/hypoxia configurations assayed. Highlighting the molecular pathways involved in GBM resistance following hypoxia and ionizing radiation (IR), this work could suggest new molecular targets, allowing the development of targeted drugs to be suggested in association with PT. |
format | Online Article Text |
id | pubmed-8073933 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80739332021-04-27 Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line Bravatà, Valentina Tinganelli, Walter Cammarata, Francesco P. Minafra, Luigi Calvaruso, Marco Sokol, Olga Petringa, Giada Cirrone, Giuseppe A.P. Scifoni, Emanuele Forte, Giusi I. Russo, Giorgio J Pers Med Article In Glioblastoma Multiforme (GBM), hypoxia is associated with radioresistance and poor prognosis. Since standard GBM treatments are not always effective, new strategies are needed to overcome resistance to therapeutic treatments, including radiotherapy (RT). Our study aims to shed light on the biomarker network involved in a hypoxic (0.2% oxygen) GBM cell line that is radioresistant after proton therapy (PT). For cultivating cells in acute hypoxia, GSI’s hypoxic chambers were used. Cells were irradiated in the middle of a spread-out Bragg peak with increasing PT doses to verify the greater radioresistance in hypoxic conditions. Whole-genome cDNA microarray gene expression analyses were performed for samples treated with 2 and 10 Gy to highlight biological processes activated in GBM following PT in the hypoxic condition. We describe cell survival response and significant deregulated pathways responsible for the cell death/survival balance and gene signatures linked to the PT/hypoxia configurations assayed. Highlighting the molecular pathways involved in GBM resistance following hypoxia and ionizing radiation (IR), this work could suggest new molecular targets, allowing the development of targeted drugs to be suggested in association with PT. MDPI 2021-04-16 /pmc/articles/PMC8073933/ /pubmed/33923454 http://dx.doi.org/10.3390/jpm11040308 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bravatà, Valentina Tinganelli, Walter Cammarata, Francesco P. Minafra, Luigi Calvaruso, Marco Sokol, Olga Petringa, Giada Cirrone, Giuseppe A.P. Scifoni, Emanuele Forte, Giusi I. Russo, Giorgio Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line |
title | Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line |
title_full | Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line |
title_fullStr | Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line |
title_full_unstemmed | Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line |
title_short | Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line |
title_sort | hypoxia transcriptomic modifications induced by proton irradiation in u87 glioblastoma multiforme cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073933/ https://www.ncbi.nlm.nih.gov/pubmed/33923454 http://dx.doi.org/10.3390/jpm11040308 |
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