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Coordinated and Independent Roles for MLH Subunits in DNA Repair
The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074049/ https://www.ncbi.nlm.nih.gov/pubmed/33923939 http://dx.doi.org/10.3390/cells10040948 |
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author | Pannafino, Gianno Alani, Eric |
author_facet | Pannafino, Gianno Alani, Eric |
author_sort | Pannafino, Gianno |
collection | PubMed |
description | The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s yeast PMS1) and MLH3 acting independently of human MLH1 in the repair of somatic double-strand breaks questions the assumption that MLH1 is an obligate subunit for MLH function. Here we provide a summary of the canonical roles for MLH factors in DNA genomic maintenance and in meiotic crossover. We then present the phenotypes of cells lacking specific MLH subunits, particularly in meiotic recombination, and based on this analysis, propose a model for an independent early role for MLH3 in meiosis to promote the accurate segregation of homologous chromosomes in the meiosis I division. |
format | Online Article Text |
id | pubmed-8074049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80740492021-04-27 Coordinated and Independent Roles for MLH Subunits in DNA Repair Pannafino, Gianno Alani, Eric Cells Review The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s yeast PMS1) and MLH3 acting independently of human MLH1 in the repair of somatic double-strand breaks questions the assumption that MLH1 is an obligate subunit for MLH function. Here we provide a summary of the canonical roles for MLH factors in DNA genomic maintenance and in meiotic crossover. We then present the phenotypes of cells lacking specific MLH subunits, particularly in meiotic recombination, and based on this analysis, propose a model for an independent early role for MLH3 in meiosis to promote the accurate segregation of homologous chromosomes in the meiosis I division. MDPI 2021-04-20 /pmc/articles/PMC8074049/ /pubmed/33923939 http://dx.doi.org/10.3390/cells10040948 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pannafino, Gianno Alani, Eric Coordinated and Independent Roles for MLH Subunits in DNA Repair |
title | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
title_full | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
title_fullStr | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
title_full_unstemmed | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
title_short | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
title_sort | coordinated and independent roles for mlh subunits in dna repair |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074049/ https://www.ncbi.nlm.nih.gov/pubmed/33923939 http://dx.doi.org/10.3390/cells10040948 |
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