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Coordinated and Independent Roles for MLH Subunits in DNA Repair

The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s...

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Autores principales: Pannafino, Gianno, Alani, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074049/
https://www.ncbi.nlm.nih.gov/pubmed/33923939
http://dx.doi.org/10.3390/cells10040948
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author Pannafino, Gianno
Alani, Eric
author_facet Pannafino, Gianno
Alani, Eric
author_sort Pannafino, Gianno
collection PubMed
description The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s yeast PMS1) and MLH3 acting independently of human MLH1 in the repair of somatic double-strand breaks questions the assumption that MLH1 is an obligate subunit for MLH function. Here we provide a summary of the canonical roles for MLH factors in DNA genomic maintenance and in meiotic crossover. We then present the phenotypes of cells lacking specific MLH subunits, particularly in meiotic recombination, and based on this analysis, propose a model for an independent early role for MLH3 in meiosis to promote the accurate segregation of homologous chromosomes in the meiosis I division.
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spelling pubmed-80740492021-04-27 Coordinated and Independent Roles for MLH Subunits in DNA Repair Pannafino, Gianno Alani, Eric Cells Review The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s yeast PMS1) and MLH3 acting independently of human MLH1 in the repair of somatic double-strand breaks questions the assumption that MLH1 is an obligate subunit for MLH function. Here we provide a summary of the canonical roles for MLH factors in DNA genomic maintenance and in meiotic crossover. We then present the phenotypes of cells lacking specific MLH subunits, particularly in meiotic recombination, and based on this analysis, propose a model for an independent early role for MLH3 in meiosis to promote the accurate segregation of homologous chromosomes in the meiosis I division. MDPI 2021-04-20 /pmc/articles/PMC8074049/ /pubmed/33923939 http://dx.doi.org/10.3390/cells10040948 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pannafino, Gianno
Alani, Eric
Coordinated and Independent Roles for MLH Subunits in DNA Repair
title Coordinated and Independent Roles for MLH Subunits in DNA Repair
title_full Coordinated and Independent Roles for MLH Subunits in DNA Repair
title_fullStr Coordinated and Independent Roles for MLH Subunits in DNA Repair
title_full_unstemmed Coordinated and Independent Roles for MLH Subunits in DNA Repair
title_short Coordinated and Independent Roles for MLH Subunits in DNA Repair
title_sort coordinated and independent roles for mlh subunits in dna repair
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074049/
https://www.ncbi.nlm.nih.gov/pubmed/33923939
http://dx.doi.org/10.3390/cells10040948
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