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Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis

Viruses have developed direct cell-to-cell transfer strategies to enter target cells without being released to escape host immune responses and antiviral treatments. These strategies are more rapid and efficient than transmission through indirect mechanisms of viral infection between cells. Here, we...

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Autores principales: Kongsomros, Supasek, Manopwisedjaroen, Suwimon, Chaopreecha, Jarinya, Wang, Sheng-Fan, Borwornpinyo, Suparerk, Thitithanyanont, Arunee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074074/
https://www.ncbi.nlm.nih.gov/pubmed/33923524
http://dx.doi.org/10.3390/pathogens10040483
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author Kongsomros, Supasek
Manopwisedjaroen, Suwimon
Chaopreecha, Jarinya
Wang, Sheng-Fan
Borwornpinyo, Suparerk
Thitithanyanont, Arunee
author_facet Kongsomros, Supasek
Manopwisedjaroen, Suwimon
Chaopreecha, Jarinya
Wang, Sheng-Fan
Borwornpinyo, Suparerk
Thitithanyanont, Arunee
author_sort Kongsomros, Supasek
collection PubMed
description Viruses have developed direct cell-to-cell transfer strategies to enter target cells without being released to escape host immune responses and antiviral treatments. These strategies are more rapid and efficient than transmission through indirect mechanisms of viral infection between cells. Here, we demonstrate that an H5N1 influenza virus can spread via direct cell-to-cell transfer in Madin-Darby canine kidney (MDCK) cells. We compared cell-to-cell transmission of the H5N1 virus to that of a human influenza H1N1 virus. The H5N1 virus has been found to spread to recipient cells faster than the human influenza H1N1 virus. Additionally, we showed that plasma membrane exchange (trogocytosis) occurs between co-cultured infected donor cells and uninfected recipient cells early point, allowing the intercellular transfer of viral material to recipient cells. Notably, the H5N1 virus induced higher trogocytosis levels than the H1N1 virus, which could explain the faster cell-to-cell transmission rate of H5N1. Importantly, this phenomenon was also observed in A549 human lung epithelial cells, which are representative cells in the natural infection site. Altogether, our results provide evidence demonstrating that trogocytosis could be the additional mechanism utilized by the H5N1 virus for rapid and efficient cell-to-cell transmission.
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spelling pubmed-80740742021-04-27 Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis Kongsomros, Supasek Manopwisedjaroen, Suwimon Chaopreecha, Jarinya Wang, Sheng-Fan Borwornpinyo, Suparerk Thitithanyanont, Arunee Pathogens Article Viruses have developed direct cell-to-cell transfer strategies to enter target cells without being released to escape host immune responses and antiviral treatments. These strategies are more rapid and efficient than transmission through indirect mechanisms of viral infection between cells. Here, we demonstrate that an H5N1 influenza virus can spread via direct cell-to-cell transfer in Madin-Darby canine kidney (MDCK) cells. We compared cell-to-cell transmission of the H5N1 virus to that of a human influenza H1N1 virus. The H5N1 virus has been found to spread to recipient cells faster than the human influenza H1N1 virus. Additionally, we showed that plasma membrane exchange (trogocytosis) occurs between co-cultured infected donor cells and uninfected recipient cells early point, allowing the intercellular transfer of viral material to recipient cells. Notably, the H5N1 virus induced higher trogocytosis levels than the H1N1 virus, which could explain the faster cell-to-cell transmission rate of H5N1. Importantly, this phenomenon was also observed in A549 human lung epithelial cells, which are representative cells in the natural infection site. Altogether, our results provide evidence demonstrating that trogocytosis could be the additional mechanism utilized by the H5N1 virus for rapid and efficient cell-to-cell transmission. MDPI 2021-04-16 /pmc/articles/PMC8074074/ /pubmed/33923524 http://dx.doi.org/10.3390/pathogens10040483 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kongsomros, Supasek
Manopwisedjaroen, Suwimon
Chaopreecha, Jarinya
Wang, Sheng-Fan
Borwornpinyo, Suparerk
Thitithanyanont, Arunee
Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis
title Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis
title_full Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis
title_fullStr Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis
title_full_unstemmed Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis
title_short Rapid and Efficient Cell-to-Cell Transmission of Avian Influenza H5N1 Virus in MDCK Cells Is Achieved by Trogocytosis
title_sort rapid and efficient cell-to-cell transmission of avian influenza h5n1 virus in mdck cells is achieved by trogocytosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074074/
https://www.ncbi.nlm.nih.gov/pubmed/33923524
http://dx.doi.org/10.3390/pathogens10040483
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