A New Approach to Quantifying Bioaccumulation of Elements in Biological Processes

SIMPLE SUMMARY: The bioaccumulation of elements (e.g., heavy metals) in living organisms (e.g., animals) is vitally important from at least two points of view: the growth and development of the organisms themselves and remediation of the polluted environment. So far, bioaccumulation has been characterized by the bioaccumulation factor (BAF), which is the ratio between the concentration of elements in the organism to the concentration in the matrix (water, soil, etc.). This factor is a good measure of bioaccumulation in ecosystems in which an organism lives from the beginning of their lives to the moment of investigation. However, especially in laboratory experiments, when organisms at a given stage of development are introduced to the system and contain some non-zero concentration of an element, the BAF can lead to misinterpretation. Therefore, we propose a new measure called the bioaccumulation index (BAI), which is the relative increase in the concentration of a given element in the organism to its initial concentration after the experiment. We proved, on the basis of data published by other authors, that the BAI was much more valid for the interpretation of bioaccumulation in these cases. ABSTRACT: Bioaccumulation, expressed as the bioaccumulation factor (BAF), is a phenomenon widely investigated in the natural environment and at laboratory scale. However, the BAF is more suitable for ecological studies, while in small-scale experiments it has limitations, which are discussed in this article. We propose a new indicator, the bioaccumulation index (BAI). The BAI takes into account the initial load of test elements, which are added to the experimental system together with the biomass of the organism. This offers the opportunity to explore the phenomena related to the bioaccumulation and, contrary to the BAF, can also reveal the dilution of element concentration in the organism. The BAF can overestimate bioaccumulation, and in an extremal situation, when the dilution of element concentration during organism growth occurs, the BAF may produce completely opposite results to the BAI. In one of the examples presented in this work (Tschirner and Simon, 2015), the concentration of phosphorous in fly larvae was lower after the experiment than in the younger larvae before the experiment. Because the phosphorous concentration in the feed was low, the BAF indicated a high bioaccumulation of this element (BAF = 14.85). In contrast, the BAI showed element dilution, which is a more realistic situation (BAI = −0.32). By taking more data into account, the BAI seems to be more valid in determining bioaccumulation, especially in the context of entomoremediation research.