Cargando…

Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds

Leymus chinensis is a perennial forage grass that has good palatability, high yield and high feed value, but seed dormancy is a major problem limiting the widespread cultivation of L. chinensis. Here, we performed transcriptomic and metabolomic analysis of hulled and de-hulled seeds of L. chinensis...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Bing, Zhang, Pan, Wang, Fengdan, Li, Ran, Liu, Jian, Wang, Qiannan, Liu, Wei, Wang, Bo, Hu, Guofu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074249/
https://www.ncbi.nlm.nih.gov/pubmed/33920519
http://dx.doi.org/10.3390/ijms22084161
_version_ 1783684312350064640
author Li, Bing
Zhang, Pan
Wang, Fengdan
Li, Ran
Liu, Jian
Wang, Qiannan
Liu, Wei
Wang, Bo
Hu, Guofu
author_facet Li, Bing
Zhang, Pan
Wang, Fengdan
Li, Ran
Liu, Jian
Wang, Qiannan
Liu, Wei
Wang, Bo
Hu, Guofu
author_sort Li, Bing
collection PubMed
description Leymus chinensis is a perennial forage grass that has good palatability, high yield and high feed value, but seed dormancy is a major problem limiting the widespread cultivation of L. chinensis. Here, we performed transcriptomic and metabolomic analysis of hulled and de-hulled seeds of L. chinensis treated with or without GA(3) to investigate the changes in gene and metabolites associated with dormancy release induced by GA(3). The germination test revealed that the optimum concentration of GA(3) for disruption of L. chinensis seed dormancy was 577 μM. A total of 4327 and 11,919 differentially expressed genes (DEGs) and 871 and 650 differentially abundant metabolites were identified in de-hulled and hulled seeds treated with GA(3), respectively, compared with seeds soaked in sterile water. Most of the DEGs were associated with starch and sucrose metabolism, protein processing in the endoplasmic reticulum, endocytosis and ribosomes. Furthermore, isoquinoline alkaloid biosynthesis, tyrosine metabolism, starch and sucrose metabolism, arginine and proline metabolism, and amino sugar and nucleotide sugar metabolism were significantly enriched pathways. Integrative analysis of the transcriptomic and metabolomic data revealed that starch and sucrose metabolism is one of the most important pathways that may play a key role in providing carbon skeletons and energy supply for the transition of L. chinensis seeds from a dormant state to germination by suppressing the expression of Cel61a, egID, cel1, tpsA, SPAC2E11.16c and TPP2, enhancing the expression of AMY1.1, AMY1.2, AMY1.6 and GLIP5, and inhibiting the synthesis of cellobiose, cellodextrin, and trehalose while promoting the hydrolysis of sucrose, starch, cellobiose, cellodextrin, and trehalose to glucose. This study identified several key genes and provided new insights into the molecular mechanism of seed dormancy release induced by GA(3) in L. chinensis. These putative genes will be valuable resources for improving the seed germination rate in future breeding studies.
format Online
Article
Text
id pubmed-8074249
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80742492021-04-27 Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds Li, Bing Zhang, Pan Wang, Fengdan Li, Ran Liu, Jian Wang, Qiannan Liu, Wei Wang, Bo Hu, Guofu Int J Mol Sci Article Leymus chinensis is a perennial forage grass that has good palatability, high yield and high feed value, but seed dormancy is a major problem limiting the widespread cultivation of L. chinensis. Here, we performed transcriptomic and metabolomic analysis of hulled and de-hulled seeds of L. chinensis treated with or without GA(3) to investigate the changes in gene and metabolites associated with dormancy release induced by GA(3). The germination test revealed that the optimum concentration of GA(3) for disruption of L. chinensis seed dormancy was 577 μM. A total of 4327 and 11,919 differentially expressed genes (DEGs) and 871 and 650 differentially abundant metabolites were identified in de-hulled and hulled seeds treated with GA(3), respectively, compared with seeds soaked in sterile water. Most of the DEGs were associated with starch and sucrose metabolism, protein processing in the endoplasmic reticulum, endocytosis and ribosomes. Furthermore, isoquinoline alkaloid biosynthesis, tyrosine metabolism, starch and sucrose metabolism, arginine and proline metabolism, and amino sugar and nucleotide sugar metabolism were significantly enriched pathways. Integrative analysis of the transcriptomic and metabolomic data revealed that starch and sucrose metabolism is one of the most important pathways that may play a key role in providing carbon skeletons and energy supply for the transition of L. chinensis seeds from a dormant state to germination by suppressing the expression of Cel61a, egID, cel1, tpsA, SPAC2E11.16c and TPP2, enhancing the expression of AMY1.1, AMY1.2, AMY1.6 and GLIP5, and inhibiting the synthesis of cellobiose, cellodextrin, and trehalose while promoting the hydrolysis of sucrose, starch, cellobiose, cellodextrin, and trehalose to glucose. This study identified several key genes and provided new insights into the molecular mechanism of seed dormancy release induced by GA(3) in L. chinensis. These putative genes will be valuable resources for improving the seed germination rate in future breeding studies. MDPI 2021-04-17 /pmc/articles/PMC8074249/ /pubmed/33920519 http://dx.doi.org/10.3390/ijms22084161 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Bing
Zhang, Pan
Wang, Fengdan
Li, Ran
Liu, Jian
Wang, Qiannan
Liu, Wei
Wang, Bo
Hu, Guofu
Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds
title Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds
title_full Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds
title_fullStr Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds
title_full_unstemmed Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds
title_short Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA(3)-Induced Dormancy Release in Leymus chinensis Seeds
title_sort integrated analysis of the transcriptome and metabolome revealed candidate genes involved in ga(3)-induced dormancy release in leymus chinensis seeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074249/
https://www.ncbi.nlm.nih.gov/pubmed/33920519
http://dx.doi.org/10.3390/ijms22084161
work_keys_str_mv AT libing integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT zhangpan integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT wangfengdan integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT liran integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT liujian integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT wangqiannan integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT liuwei integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT wangbo integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds
AT huguofu integratedanalysisofthetranscriptomeandmetabolomerevealedcandidategenesinvolvedinga3induceddormancyreleaseinleymuschinensisseeds