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Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests

The 2019 Coronavirus disease (COVID-19) outbreak had detrimental effects on essential medical services such as organ and tissue donation. Lombardy, one of the most active Italian regions in organ/tissue procurement, has been strongly affected by the COVID-19 pandemic. To date, data concerning the ri...

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Autores principales: Perrucci, Gianluca Lorenzo, Sommariva, Elena, Ricci, Veronica, Songia, Paola, D’Alessandra, Yuri, Poggio, Paolo, Pompilio, Giulio, Polvani, Gianluca, Guarino, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074260/
https://www.ncbi.nlm.nih.gov/pubmed/33924082
http://dx.doi.org/10.3390/diagnostics11040731
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author Perrucci, Gianluca Lorenzo
Sommariva, Elena
Ricci, Veronica
Songia, Paola
D’Alessandra, Yuri
Poggio, Paolo
Pompilio, Giulio
Polvani, Gianluca
Guarino, Anna
author_facet Perrucci, Gianluca Lorenzo
Sommariva, Elena
Ricci, Veronica
Songia, Paola
D’Alessandra, Yuri
Poggio, Paolo
Pompilio, Giulio
Polvani, Gianluca
Guarino, Anna
author_sort Perrucci, Gianluca Lorenzo
collection PubMed
description The 2019 Coronavirus disease (COVID-19) outbreak had detrimental effects on essential medical services such as organ and tissue donation. Lombardy, one of the most active Italian regions in organ/tissue procurement, has been strongly affected by the COVID-19 pandemic. To date, data concerning the risk of SARS-CoV-2 transmission after tissue transplantation are controversial. Here, we aimed to evaluate the presence/absence of SARS-CoV-2 in different cardiac tissues eligible for transplantation obtained from Lombard donors. We used cardiovascular tissues from eight donors potentially suitable for pulmonary valve transplantation. All donor subjects involved in the study returned negative results for the SARS-CoV-2 RNA molecular tests (quantitative real-time reverse-transcription PCR, qRT-PCR, and chip-based digital PCR) in nasopharyngeal swabs (NPS) or bronchoalveolar lavage (BAL). None of the eight donors included in this study revealed the presence of the SARS-CoV-2 viral genome. However, evaluation of the protein content of pulmonary vein wall (PVW) tissue revealed variable levels of SARS-CoV-2 nucleoprotein signal in all donors. Our study demonstrated for the first time, to the best of our knowledge, that viral nucleoprotein but not viral RNA was present in the examined tissue bank specimens, suggesting the need for caution and in-depth investigations on implantable tissue specimens collected during the COVID-19 pandemic period.
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spelling pubmed-80742602021-04-27 Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests Perrucci, Gianluca Lorenzo Sommariva, Elena Ricci, Veronica Songia, Paola D’Alessandra, Yuri Poggio, Paolo Pompilio, Giulio Polvani, Gianluca Guarino, Anna Diagnostics (Basel) Communication The 2019 Coronavirus disease (COVID-19) outbreak had detrimental effects on essential medical services such as organ and tissue donation. Lombardy, one of the most active Italian regions in organ/tissue procurement, has been strongly affected by the COVID-19 pandemic. To date, data concerning the risk of SARS-CoV-2 transmission after tissue transplantation are controversial. Here, we aimed to evaluate the presence/absence of SARS-CoV-2 in different cardiac tissues eligible for transplantation obtained from Lombard donors. We used cardiovascular tissues from eight donors potentially suitable for pulmonary valve transplantation. All donor subjects involved in the study returned negative results for the SARS-CoV-2 RNA molecular tests (quantitative real-time reverse-transcription PCR, qRT-PCR, and chip-based digital PCR) in nasopharyngeal swabs (NPS) or bronchoalveolar lavage (BAL). None of the eight donors included in this study revealed the presence of the SARS-CoV-2 viral genome. However, evaluation of the protein content of pulmonary vein wall (PVW) tissue revealed variable levels of SARS-CoV-2 nucleoprotein signal in all donors. Our study demonstrated for the first time, to the best of our knowledge, that viral nucleoprotein but not viral RNA was present in the examined tissue bank specimens, suggesting the need for caution and in-depth investigations on implantable tissue specimens collected during the COVID-19 pandemic period. MDPI 2021-04-20 /pmc/articles/PMC8074260/ /pubmed/33924082 http://dx.doi.org/10.3390/diagnostics11040731 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Perrucci, Gianluca Lorenzo
Sommariva, Elena
Ricci, Veronica
Songia, Paola
D’Alessandra, Yuri
Poggio, Paolo
Pompilio, Giulio
Polvani, Gianluca
Guarino, Anna
Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests
title Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests
title_full Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests
title_fullStr Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests
title_full_unstemmed Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests
title_short Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests
title_sort presence of sars-cov-2 nucleoprotein in cardiac tissues of donors with negative covid-19 molecular tests
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074260/
https://www.ncbi.nlm.nih.gov/pubmed/33924082
http://dx.doi.org/10.3390/diagnostics11040731
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