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Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs
Ambroxol (Ax) is used as a mucolytics in the treatment of respiratory tract infections. Ax, at a general dose for humans, does not alter Chlamydia pneumoniae growth in mice. Therefore, we aimed to investigate the potential anti-chlamydial effect of Ax at a concentration four timed higher than that u...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074272/ https://www.ncbi.nlm.nih.gov/pubmed/33924075 http://dx.doi.org/10.3390/microorganisms9040880 |
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author | Kókai, Dávid Paróczai, Dóra Virok, Dezső Peter Endrész, Valéria Gáspár, Renáta Csont, Tamás Bozó, Renáta Burián, Katalin |
author_facet | Kókai, Dávid Paróczai, Dóra Virok, Dezső Peter Endrész, Valéria Gáspár, Renáta Csont, Tamás Bozó, Renáta Burián, Katalin |
author_sort | Kókai, Dávid |
collection | PubMed |
description | Ambroxol (Ax) is used as a mucolytics in the treatment of respiratory tract infections. Ax, at a general dose for humans, does not alter Chlamydia pneumoniae growth in mice. Therefore, we aimed to investigate the potential anti-chlamydial effect of Ax at a concentration four timed higher than that used in human medicine. Mice were infected with C. pneumoniae and 5-mg/kg Ax was administered orally. The number of recoverable C. pneumoniae inclusion-forming units (IFUs) in Ax-treated mice was significantly lower than that in untreated mice. mRNA expression levels of several cytokines, including interleukin 12 (IL-12), IL-23, IL-17F, interferon gamma (IFN-γ), and surfactant protein (SP)-A, increased in infected mice treated with Ax. The IFN-γ protein expression levels were also significantly higher in infected and Ax-treated mice. Furthermore, the in vitro results suggested that the ERK 1/2 activity was decreased, which is essential for the C. pneumoniae replication. SP-A and SP-D treatments significantly decreased the number of viable C. pneumoniae IFUs and significantly increased the attachment of C. pneumoniae to macrophage cells. Based on our results, a dose of 5 mg/kg of Ax exhibited an anti-chlamydial effect in mice, probably an immunomodulating effect, and may be used as supporting drug in respiratory infections caused by C. pneumoniae. |
format | Online Article Text |
id | pubmed-8074272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80742722021-04-27 Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs Kókai, Dávid Paróczai, Dóra Virok, Dezső Peter Endrész, Valéria Gáspár, Renáta Csont, Tamás Bozó, Renáta Burián, Katalin Microorganisms Article Ambroxol (Ax) is used as a mucolytics in the treatment of respiratory tract infections. Ax, at a general dose for humans, does not alter Chlamydia pneumoniae growth in mice. Therefore, we aimed to investigate the potential anti-chlamydial effect of Ax at a concentration four timed higher than that used in human medicine. Mice were infected with C. pneumoniae and 5-mg/kg Ax was administered orally. The number of recoverable C. pneumoniae inclusion-forming units (IFUs) in Ax-treated mice was significantly lower than that in untreated mice. mRNA expression levels of several cytokines, including interleukin 12 (IL-12), IL-23, IL-17F, interferon gamma (IFN-γ), and surfactant protein (SP)-A, increased in infected mice treated with Ax. The IFN-γ protein expression levels were also significantly higher in infected and Ax-treated mice. Furthermore, the in vitro results suggested that the ERK 1/2 activity was decreased, which is essential for the C. pneumoniae replication. SP-A and SP-D treatments significantly decreased the number of viable C. pneumoniae IFUs and significantly increased the attachment of C. pneumoniae to macrophage cells. Based on our results, a dose of 5 mg/kg of Ax exhibited an anti-chlamydial effect in mice, probably an immunomodulating effect, and may be used as supporting drug in respiratory infections caused by C. pneumoniae. MDPI 2021-04-20 /pmc/articles/PMC8074272/ /pubmed/33924075 http://dx.doi.org/10.3390/microorganisms9040880 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kókai, Dávid Paróczai, Dóra Virok, Dezső Peter Endrész, Valéria Gáspár, Renáta Csont, Tamás Bozó, Renáta Burián, Katalin Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs |
title | Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs |
title_full | Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs |
title_fullStr | Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs |
title_full_unstemmed | Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs |
title_short | Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs |
title_sort | ambroxol treatment suppresses the proliferation of chlamydia pneumoniae in murine lungs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074272/ https://www.ncbi.nlm.nih.gov/pubmed/33924075 http://dx.doi.org/10.3390/microorganisms9040880 |
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