Cargando…

Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols

Bovine alphaherpesvirus 1 is ubiquitous in cattle populations and is associated with several clinical syndromes, including respiratory disease, genital disease, infertility and abortions. Control of the virus in many parts of the world is achieved primarily through vaccination with either inactivate...

Descripción completa

Detalles Bibliográficos
Autores principales: De Brun, Laureana, Leites, Mauro, Furtado, Agustín, Campos, Fabricio, Roehe, Paulo, Puentes, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074307/
https://www.ncbi.nlm.nih.gov/pubmed/33924141
http://dx.doi.org/10.3390/vaccines9040408
_version_ 1783684325695291392
author De Brun, Laureana
Leites, Mauro
Furtado, Agustín
Campos, Fabricio
Roehe, Paulo
Puentes, Rodrigo
author_facet De Brun, Laureana
Leites, Mauro
Furtado, Agustín
Campos, Fabricio
Roehe, Paulo
Puentes, Rodrigo
author_sort De Brun, Laureana
collection PubMed
description Bovine alphaherpesvirus 1 is ubiquitous in cattle populations and is associated with several clinical syndromes, including respiratory disease, genital disease, infertility and abortions. Control of the virus in many parts of the world is achieved primarily through vaccination with either inactivated or live modified viral vaccines. The objective of this study was to evaluate the performance of four commercially available BoHV-1 vaccines commonly used in Central and South America. Animals were divided into eight groups and vaccinated on days 0 and 30. Groups 1 to 4 received two doses of four different BoHV-1 commercial vaccines (named A to D). Groups 5 and 6 received vaccine D plus a vaccine for either Clostridial or Food-and-Mouth-Disease (FMD), respectively. Group 7 received one dose of two different brands of reproductive vaccines. Serum samples were collected from all animals on days 0, 30 and 60 to evaluate neutralizing and isotype-specific (IgG1 and IgG2) antibodies. Of the four commercial vaccines evaluated, only vaccine A induced neutralizing antibodies to titers ≥ 1:8 in 13/15 (86%) of the animals 60 days post-vaccination. Levels of IgG2 antibody increased in all groups, except for group 2 after the first dose of vaccine B. These results show that only vaccine A induced significant and detectable levels of BoHV-1-neutralizing antibodies. The combination of vaccine D with Clostridial or FMD vaccines did not affect neutralizing antibody responses to BoHV-1. The antibody responses of three of the four commercial vaccines analyzed here were lower than admissible by vaccine A. These results may be from vaccination failure, but means to identify the immune signatures predictive of clinical protection against BoHV-1 in cattle should also be considered.
format Online
Article
Text
id pubmed-8074307
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80743072021-04-27 Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols De Brun, Laureana Leites, Mauro Furtado, Agustín Campos, Fabricio Roehe, Paulo Puentes, Rodrigo Vaccines (Basel) Article Bovine alphaherpesvirus 1 is ubiquitous in cattle populations and is associated with several clinical syndromes, including respiratory disease, genital disease, infertility and abortions. Control of the virus in many parts of the world is achieved primarily through vaccination with either inactivated or live modified viral vaccines. The objective of this study was to evaluate the performance of four commercially available BoHV-1 vaccines commonly used in Central and South America. Animals were divided into eight groups and vaccinated on days 0 and 30. Groups 1 to 4 received two doses of four different BoHV-1 commercial vaccines (named A to D). Groups 5 and 6 received vaccine D plus a vaccine for either Clostridial or Food-and-Mouth-Disease (FMD), respectively. Group 7 received one dose of two different brands of reproductive vaccines. Serum samples were collected from all animals on days 0, 30 and 60 to evaluate neutralizing and isotype-specific (IgG1 and IgG2) antibodies. Of the four commercial vaccines evaluated, only vaccine A induced neutralizing antibodies to titers ≥ 1:8 in 13/15 (86%) of the animals 60 days post-vaccination. Levels of IgG2 antibody increased in all groups, except for group 2 after the first dose of vaccine B. These results show that only vaccine A induced significant and detectable levels of BoHV-1-neutralizing antibodies. The combination of vaccine D with Clostridial or FMD vaccines did not affect neutralizing antibody responses to BoHV-1. The antibody responses of three of the four commercial vaccines analyzed here were lower than admissible by vaccine A. These results may be from vaccination failure, but means to identify the immune signatures predictive of clinical protection against BoHV-1 in cattle should also be considered. MDPI 2021-04-20 /pmc/articles/PMC8074307/ /pubmed/33924141 http://dx.doi.org/10.3390/vaccines9040408 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Brun, Laureana
Leites, Mauro
Furtado, Agustín
Campos, Fabricio
Roehe, Paulo
Puentes, Rodrigo
Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols
title Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols
title_full Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols
title_fullStr Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols
title_full_unstemmed Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols
title_short Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols
title_sort field evaluation of commercial vaccines against infectious bovine rhinotracheitis (ibr) virus using different immunization protocols
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074307/
https://www.ncbi.nlm.nih.gov/pubmed/33924141
http://dx.doi.org/10.3390/vaccines9040408
work_keys_str_mv AT debrunlaureana fieldevaluationofcommercialvaccinesagainstinfectiousbovinerhinotracheitisibrvirususingdifferentimmunizationprotocols
AT leitesmauro fieldevaluationofcommercialvaccinesagainstinfectiousbovinerhinotracheitisibrvirususingdifferentimmunizationprotocols
AT furtadoagustin fieldevaluationofcommercialvaccinesagainstinfectiousbovinerhinotracheitisibrvirususingdifferentimmunizationprotocols
AT camposfabricio fieldevaluationofcommercialvaccinesagainstinfectiousbovinerhinotracheitisibrvirususingdifferentimmunizationprotocols
AT roehepaulo fieldevaluationofcommercialvaccinesagainstinfectiousbovinerhinotracheitisibrvirususingdifferentimmunizationprotocols
AT puentesrodrigo fieldevaluationofcommercialvaccinesagainstinfectiousbovinerhinotracheitisibrvirususingdifferentimmunizationprotocols