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Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents

Traditional chemo-mechanical retraction/displacement materials can impact the gingival margin tissues. This study was undertaken to analyze biological responses induced in human gingival fibroblasts (HGFs) upon application of injectable astringent-based agents used in the cordless retraction techniq...

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Autores principales: Nowakowska, Danuta, Kulbacka, Julita, Wezgowiec, Joanna, Szewczyk, Anna, Baczynska, Dagmara, Zietek, Marek, Wieckiewicz, Wlodzimierz, Saczko, Jolanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074361/
https://www.ncbi.nlm.nih.gov/pubmed/33924145
http://dx.doi.org/10.3390/ma14082081
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author Nowakowska, Danuta
Kulbacka, Julita
Wezgowiec, Joanna
Szewczyk, Anna
Baczynska, Dagmara
Zietek, Marek
Wieckiewicz, Wlodzimierz
Saczko, Jolanta
author_facet Nowakowska, Danuta
Kulbacka, Julita
Wezgowiec, Joanna
Szewczyk, Anna
Baczynska, Dagmara
Zietek, Marek
Wieckiewicz, Wlodzimierz
Saczko, Jolanta
author_sort Nowakowska, Danuta
collection PubMed
description Traditional chemo-mechanical retraction/displacement materials can impact the gingival margin tissues. This study was undertaken to analyze biological responses induced in human gingival fibroblasts (HGFs) upon application of injectable astringent-based agents used in the cordless retraction technique. HGFs were exposed to hemostatic agents (five gels, three pastes, and one foam) based on aluminium chloride, aluminium sulphate and ferric sulphate. Changes in cell viability and proliferation were evaluated using an MTT assay and a BrdU assay. The cytoskeleton structure organization (zyxin and F-actin) was visualized by confocal laser scanning microscopy. Oxidative stress was determined using the Griess Reagent System. The RNA expression levels of antioxidant enzymes were quantified by real-time RT-PCR. The statistical significance was evaluated using Student’s t-test and one-way ANOVA with post-hoc Tukey HSD test. The evaluated agents did not downregulate fibroblast viability or proliferation. No significant cytoskeleton reorganization was observed. Only one agent (Expasyl) induced oxidative stress, demonstrated by the increased level of nitrites. Incubation with the studied agents significantly increased the RNA expression of some antioxidant enzymes (SOD1, SOD3, GPX1). However, no significant influence on the expression of SOD2 and HMOX1 was detected. The injectable forms of chemical retraction agents revealed biocompatibility with HGFs, suggesting their potential clinical usefulness in gingival margin retraction.
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spelling pubmed-80743612021-04-27 Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents Nowakowska, Danuta Kulbacka, Julita Wezgowiec, Joanna Szewczyk, Anna Baczynska, Dagmara Zietek, Marek Wieckiewicz, Wlodzimierz Saczko, Jolanta Materials (Basel) Article Traditional chemo-mechanical retraction/displacement materials can impact the gingival margin tissues. This study was undertaken to analyze biological responses induced in human gingival fibroblasts (HGFs) upon application of injectable astringent-based agents used in the cordless retraction technique. HGFs were exposed to hemostatic agents (five gels, three pastes, and one foam) based on aluminium chloride, aluminium sulphate and ferric sulphate. Changes in cell viability and proliferation were evaluated using an MTT assay and a BrdU assay. The cytoskeleton structure organization (zyxin and F-actin) was visualized by confocal laser scanning microscopy. Oxidative stress was determined using the Griess Reagent System. The RNA expression levels of antioxidant enzymes were quantified by real-time RT-PCR. The statistical significance was evaluated using Student’s t-test and one-way ANOVA with post-hoc Tukey HSD test. The evaluated agents did not downregulate fibroblast viability or proliferation. No significant cytoskeleton reorganization was observed. Only one agent (Expasyl) induced oxidative stress, demonstrated by the increased level of nitrites. Incubation with the studied agents significantly increased the RNA expression of some antioxidant enzymes (SOD1, SOD3, GPX1). However, no significant influence on the expression of SOD2 and HMOX1 was detected. The injectable forms of chemical retraction agents revealed biocompatibility with HGFs, suggesting their potential clinical usefulness in gingival margin retraction. MDPI 2021-04-20 /pmc/articles/PMC8074361/ /pubmed/33924145 http://dx.doi.org/10.3390/ma14082081 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nowakowska, Danuta
Kulbacka, Julita
Wezgowiec, Joanna
Szewczyk, Anna
Baczynska, Dagmara
Zietek, Marek
Wieckiewicz, Wlodzimierz
Saczko, Jolanta
Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents
title Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents
title_full Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents
title_fullStr Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents
title_full_unstemmed Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents
title_short Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents
title_sort biological response induced in primary human gingival fibroblasts upon exposure to various types of injectable astringent retraction agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074361/
https://www.ncbi.nlm.nih.gov/pubmed/33924145
http://dx.doi.org/10.3390/ma14082081
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