Game-Changing Approaches in Sperm Sex-Sorting: Microfluidics and Nanotechnology

SIMPLE SUMMARY: Sexing of sperm cells, including the capacity to preselect the sex of offspring prior to reproduction, has been a major target of reproductive biotechnology for a very long time. The advances in molecular biology, biophysics, and computer science over the past few decades, as well as the groundbreaking new methods introduced by scientists, have contributed to some major breakthroughs in a variety of branches of medicine. In particular, assisted reproduction is one of the areas in which emerging technologies such as nanotechnology and microfluidics may enhance the fertility potential of samples of sex-sorted semen, thus improving the reproductive management of farm animals and conservation programs. In human medicine, embryo sex-selection using in vitro fertilization (IVF) and preimplantation genetic testing (PGT) is accepted only for medical reasons. Using sex-sorting before IVF would enable specialists to prevent sex-linked genetic diseases and prevent the discharge of embryos which are not suitable for transfer due to their sex. ABSTRACT: The utilization of sex-sorted sperm for artificial insemination and in-vitro fertilization is considered a valuable tool for improving production efficiency and optimizing reproductive management in farm animals, subsequently ensuring sufficient food resource for the growing human population. Despite the fact that sperm sex-sorting is one of the most intense studied technologies and notable progress have been made in the past three decades to optimize it, the conception rates when using sex-sorted semen are still under expectations. Assisted reproduction programs may benefit from the use of emergent nano and microfluidic-based technologies. This article addresses the currently used methods for sperm sex-sorting, as well as the emerging ones, based on nanotechnology and microfluidics emphasizing on their practical and economic applicability.