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An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin
Although the largely positive intramembrane dipole potential (DP) may substantially influence the function of transmembrane proteins, its investigation is deeply hampered by the lack of measurement techniques suitable for high-throughput examination of living cells. Here, we describe a novel emissio...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074792/ https://www.ncbi.nlm.nih.gov/pubmed/33912562 http://dx.doi.org/10.3389/fcell.2021.647300 |
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author | Zakany, Florina Szabo, Mate Batta, Gyula Kárpáti, Levente Mándity, István M. Fülöp, Péter Varga, Zoltan Panyi, Gyorgy Nagy, Peter Kovacs, Tamas |
author_facet | Zakany, Florina Szabo, Mate Batta, Gyula Kárpáti, Levente Mándity, István M. Fülöp, Péter Varga, Zoltan Panyi, Gyorgy Nagy, Peter Kovacs, Tamas |
author_sort | Zakany, Florina |
collection | PubMed |
description | Although the largely positive intramembrane dipole potential (DP) may substantially influence the function of transmembrane proteins, its investigation is deeply hampered by the lack of measurement techniques suitable for high-throughput examination of living cells. Here, we describe a novel emission ratiometric flow cytometry method based on F66, a 3-hydroxiflavon derivative, and demonstrate that 6-ketocholestanol, cholesterol and 7-dehydrocholesterol, saturated stearic acid (SA) and ω-6 γ-linolenic acid (GLA) increase, while ω-3 α-linolenic acid (ALA) decreases the DP. These changes do not correlate with alterations in cell viability or membrane fluidity. Pretreatment with ALA counteracts, while SA or GLA enhances cholesterol-induced DP elevations. Furthermore, ALA (but not SA or GLA) increases endo-lysosomal escape of penetratin, a cell-penetrating peptide. In summary, we have developed a novel method to measure DP in large quantities of individual living cells and propose ALA as a physiological DP lowering agent facilitating cytoplasmic entry of penetratin. |
format | Online Article Text |
id | pubmed-8074792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80747922021-04-27 An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin Zakany, Florina Szabo, Mate Batta, Gyula Kárpáti, Levente Mándity, István M. Fülöp, Péter Varga, Zoltan Panyi, Gyorgy Nagy, Peter Kovacs, Tamas Front Cell Dev Biol Cell and Developmental Biology Although the largely positive intramembrane dipole potential (DP) may substantially influence the function of transmembrane proteins, its investigation is deeply hampered by the lack of measurement techniques suitable for high-throughput examination of living cells. Here, we describe a novel emission ratiometric flow cytometry method based on F66, a 3-hydroxiflavon derivative, and demonstrate that 6-ketocholestanol, cholesterol and 7-dehydrocholesterol, saturated stearic acid (SA) and ω-6 γ-linolenic acid (GLA) increase, while ω-3 α-linolenic acid (ALA) decreases the DP. These changes do not correlate with alterations in cell viability or membrane fluidity. Pretreatment with ALA counteracts, while SA or GLA enhances cholesterol-induced DP elevations. Furthermore, ALA (but not SA or GLA) increases endo-lysosomal escape of penetratin, a cell-penetrating peptide. In summary, we have developed a novel method to measure DP in large quantities of individual living cells and propose ALA as a physiological DP lowering agent facilitating cytoplasmic entry of penetratin. Frontiers Media S.A. 2021-04-12 /pmc/articles/PMC8074792/ /pubmed/33912562 http://dx.doi.org/10.3389/fcell.2021.647300 Text en Copyright © 2021 Zakany, Szabo, Batta, Kárpáti, Mándity, Fülöp, Varga, Panyi, Nagy and Kovacs. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Zakany, Florina Szabo, Mate Batta, Gyula Kárpáti, Levente Mándity, István M. Fülöp, Péter Varga, Zoltan Panyi, Gyorgy Nagy, Peter Kovacs, Tamas An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin |
title | An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin |
title_full | An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin |
title_fullStr | An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin |
title_full_unstemmed | An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin |
title_short | An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin |
title_sort | ω-3, but not an ω-6 polyunsaturated fatty acid decreases membrane dipole potential and stimulates endo-lysosomal escape of penetratin |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074792/ https://www.ncbi.nlm.nih.gov/pubmed/33912562 http://dx.doi.org/10.3389/fcell.2021.647300 |
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