An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures

Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is wo...

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Autores principales: Ding, Ruiwen, Ying, Jianxi, Zhao, Yufen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074886/
https://www.ncbi.nlm.nih.gov/pubmed/33959351
http://dx.doi.org/10.1098/rsos.201963
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author Ding, Ruiwen
Ying, Jianxi
Zhao, Yufen
author_facet Ding, Ruiwen
Ying, Jianxi
Zhao, Yufen
author_sort Ding, Ruiwen
collection PubMed
description Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures.
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spelling pubmed-80748862021-05-05 An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures Ding, Ruiwen Ying, Jianxi Zhao, Yufen R Soc Open Sci Chemistry Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures. The Royal Society 2021-03-10 /pmc/articles/PMC8074886/ /pubmed/33959351 http://dx.doi.org/10.1098/rsos.201963 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Chemistry
Ding, Ruiwen
Ying, Jianxi
Zhao, Yufen
An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
title An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
title_full An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
title_fullStr An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
title_full_unstemmed An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
title_short An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
title_sort electronic circular dichroism spectroscopy method for the quantification of l- and d-amino acids in enantiomeric mixtures
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074886/
https://www.ncbi.nlm.nih.gov/pubmed/33959351
http://dx.doi.org/10.1098/rsos.201963
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