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Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system

Biodegradable nanoparticles have been well studied as biocompatible delivery systems. Nanoparticles of less than 200 nm in size can facilitate the passive targeting of drugs to tumour tissues and their accumulation therein via the enhanced permeability and retention (EPR) effect. Recent studies have...

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Autores principales: Lee, Sungho, Miyajima, Tatsuya, Sugawara-Narutaki, Ayae, Kato, Katsuya, Nagata, Fukue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074949/
https://www.ncbi.nlm.nih.gov/pubmed/33959355
http://dx.doi.org/10.1098/rsos.202030
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author Lee, Sungho
Miyajima, Tatsuya
Sugawara-Narutaki, Ayae
Kato, Katsuya
Nagata, Fukue
author_facet Lee, Sungho
Miyajima, Tatsuya
Sugawara-Narutaki, Ayae
Kato, Katsuya
Nagata, Fukue
author_sort Lee, Sungho
collection PubMed
description Biodegradable nanoparticles have been well studied as biocompatible delivery systems. Nanoparticles of less than 200 nm in size can facilitate the passive targeting of drugs to tumour tissues and their accumulation therein via the enhanced permeability and retention (EPR) effect. Recent studies have focused on stimuli-responsive drug delivery systems (DDS) for improving the effectiveness of chemotherapy; for example, pH-sensitive DDS depend on the weakly acidic and neutral extracellular pH of tumour and normal tissues, respectively. In our previous work, core–shell nanoparticles composed of the biodegradable polymer poly(lactic acid) (PLA) and the widely used inorganic biomaterial hydroxyapatite (HAp, which exhibits pH sensitivity) were prepared using a surfactant-free method. These PLA/HAp core–shell nanoparticles could load 750 wt% of a hydrophobic model drug. In this work, the properties of the PLA/HAp core–shell nanoparticles loaded with the anti-cancer drug paclitaxel (PTX) were thoroughly investigated in vitro. Because the PTX-containing nanoparticles were approximately 80 nm in size, they can be expected to facilitate efficient drug delivery via the EPR effect. The core–shell nanoparticles were cytotoxic towards cancer cells (4T1). This was due to the pH sensitivity of the HAp shell, which is stable in neutral conditions and dissolves in acidic conditions. The cytotoxic activity of the PTX-loaded nanoparticles was sustained for up to 48 h, which was suitable for tumour growth inhibition. These results suggest that the core–shell nanoparticles can be suitable drug carriers for various water-insoluble drugs.
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spelling pubmed-80749492021-05-05 Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system Lee, Sungho Miyajima, Tatsuya Sugawara-Narutaki, Ayae Kato, Katsuya Nagata, Fukue R Soc Open Sci Chemistry Biodegradable nanoparticles have been well studied as biocompatible delivery systems. Nanoparticles of less than 200 nm in size can facilitate the passive targeting of drugs to tumour tissues and their accumulation therein via the enhanced permeability and retention (EPR) effect. Recent studies have focused on stimuli-responsive drug delivery systems (DDS) for improving the effectiveness of chemotherapy; for example, pH-sensitive DDS depend on the weakly acidic and neutral extracellular pH of tumour and normal tissues, respectively. In our previous work, core–shell nanoparticles composed of the biodegradable polymer poly(lactic acid) (PLA) and the widely used inorganic biomaterial hydroxyapatite (HAp, which exhibits pH sensitivity) were prepared using a surfactant-free method. These PLA/HAp core–shell nanoparticles could load 750 wt% of a hydrophobic model drug. In this work, the properties of the PLA/HAp core–shell nanoparticles loaded with the anti-cancer drug paclitaxel (PTX) were thoroughly investigated in vitro. Because the PTX-containing nanoparticles were approximately 80 nm in size, they can be expected to facilitate efficient drug delivery via the EPR effect. The core–shell nanoparticles were cytotoxic towards cancer cells (4T1). This was due to the pH sensitivity of the HAp shell, which is stable in neutral conditions and dissolves in acidic conditions. The cytotoxic activity of the PTX-loaded nanoparticles was sustained for up to 48 h, which was suitable for tumour growth inhibition. These results suggest that the core–shell nanoparticles can be suitable drug carriers for various water-insoluble drugs. The Royal Society 2021-03-24 /pmc/articles/PMC8074949/ /pubmed/33959355 http://dx.doi.org/10.1098/rsos.202030 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Chemistry
Lee, Sungho
Miyajima, Tatsuya
Sugawara-Narutaki, Ayae
Kato, Katsuya
Nagata, Fukue
Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system
title Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system
title_full Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system
title_fullStr Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system
title_full_unstemmed Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system
title_short Development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system
title_sort development of paclitaxel-loaded poly(lactic acid)/hydroxyapatite core–shell nanoparticles as a stimuli-responsive drug delivery system
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074949/
https://www.ncbi.nlm.nih.gov/pubmed/33959355
http://dx.doi.org/10.1098/rsos.202030
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