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Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton

Lymphocytes rearrange their shape, membrane receptors and organelles during cognate contacts with antigen-presenting cells (APCs). Activation of T cells by APCs through pMHC-TCR/CD3 interaction (peptide-major histocompatibility complex-T cell receptor/CD3 complexes) involves different steps that lea...

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Autores principales: Martín-Cófreces, Noa Beatriz, Valpuesta, José María, Sánchez-Madrid, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075050/
https://www.ncbi.nlm.nih.gov/pubmed/33912568
http://dx.doi.org/10.3389/fcell.2021.658460
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author Martín-Cófreces, Noa Beatriz
Valpuesta, José María
Sánchez-Madrid, Francisco
author_facet Martín-Cófreces, Noa Beatriz
Valpuesta, José María
Sánchez-Madrid, Francisco
author_sort Martín-Cófreces, Noa Beatriz
collection PubMed
description Lymphocytes rearrange their shape, membrane receptors and organelles during cognate contacts with antigen-presenting cells (APCs). Activation of T cells by APCs through pMHC-TCR/CD3 interaction (peptide-major histocompatibility complex-T cell receptor/CD3 complexes) involves different steps that lead to the reorganization of the cytoskeleton and organelles and, eventually, activation of nuclear factors allowing transcription and ultimately, replication and cell division. Both the positioning of the lymphocyte centrosome in close proximity to the APC and the nucleation of a dense microtubule network beneath the plasma membrane from the centrosome support the T cell’s intracellular polarity. Signaling from the TCR is facilitated by this traffic, which constitutes an important pathway for regulation of T cell activation. The coordinated enrichment upon T cell stimulation of the chaperonin CCT (chaperonin-containing tailless complex polypeptide 1; also termed TRiC) and tubulins at the centrosome area support polarized tubulin polymerization and T cell activation. The proteasome is also enriched in the centrosome of activated T cells, providing a mechanism to balance local protein synthesis and degradation. CCT assists the folding of proteins coming from de novo synthesis, therefore favoring mRNA translation. The functional role of this chaperonin in regulating cytoskeletal composition and dynamics at the immune synapse is discussed.
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spelling pubmed-80750502021-04-27 Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton Martín-Cófreces, Noa Beatriz Valpuesta, José María Sánchez-Madrid, Francisco Front Cell Dev Biol Cell and Developmental Biology Lymphocytes rearrange their shape, membrane receptors and organelles during cognate contacts with antigen-presenting cells (APCs). Activation of T cells by APCs through pMHC-TCR/CD3 interaction (peptide-major histocompatibility complex-T cell receptor/CD3 complexes) involves different steps that lead to the reorganization of the cytoskeleton and organelles and, eventually, activation of nuclear factors allowing transcription and ultimately, replication and cell division. Both the positioning of the lymphocyte centrosome in close proximity to the APC and the nucleation of a dense microtubule network beneath the plasma membrane from the centrosome support the T cell’s intracellular polarity. Signaling from the TCR is facilitated by this traffic, which constitutes an important pathway for regulation of T cell activation. The coordinated enrichment upon T cell stimulation of the chaperonin CCT (chaperonin-containing tailless complex polypeptide 1; also termed TRiC) and tubulins at the centrosome area support polarized tubulin polymerization and T cell activation. The proteasome is also enriched in the centrosome of activated T cells, providing a mechanism to balance local protein synthesis and degradation. CCT assists the folding of proteins coming from de novo synthesis, therefore favoring mRNA translation. The functional role of this chaperonin in regulating cytoskeletal composition and dynamics at the immune synapse is discussed. Frontiers Media S.A. 2021-04-12 /pmc/articles/PMC8075050/ /pubmed/33912568 http://dx.doi.org/10.3389/fcell.2021.658460 Text en Copyright © 2021 Martín-Cófreces, Valpuesta and Sánchez-Madrid. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Martín-Cófreces, Noa Beatriz
Valpuesta, José María
Sánchez-Madrid, Francisco
Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton
title Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton
title_full Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton
title_fullStr Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton
title_full_unstemmed Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton
title_short Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton
title_sort folding for the immune synapse: cct chaperonin and the cytoskeleton
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075050/
https://www.ncbi.nlm.nih.gov/pubmed/33912568
http://dx.doi.org/10.3389/fcell.2021.658460
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