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1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling

The increasing intensity of environmental radiofrequency electromagnetic fields (RF-EMF) has increased public concern about its health effects. Of particular concern are the influences of RF-EMF exposure on the development of the brain. The mechanisms of how RF-EMF acts on the developing brain are n...

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Autores principales: Chen, Chunhai, Ma, Qinglong, Deng, Ping, Lin, Min, Gao, Peng, He, Mindi, Lu, Yonghui, Pi, Huifeng, He, Zhixin, Zhou, Chao, Zhang, Yanwen, Yu, Zhengping, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075058/
https://www.ncbi.nlm.nih.gov/pubmed/33912567
http://dx.doi.org/10.3389/fcell.2021.657623
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author Chen, Chunhai
Ma, Qinglong
Deng, Ping
Lin, Min
Gao, Peng
He, Mindi
Lu, Yonghui
Pi, Huifeng
He, Zhixin
Zhou, Chao
Zhang, Yanwen
Yu, Zhengping
Zhang, Lei
author_facet Chen, Chunhai
Ma, Qinglong
Deng, Ping
Lin, Min
Gao, Peng
He, Mindi
Lu, Yonghui
Pi, Huifeng
He, Zhixin
Zhou, Chao
Zhang, Yanwen
Yu, Zhengping
Zhang, Lei
author_sort Chen, Chunhai
collection PubMed
description The increasing intensity of environmental radiofrequency electromagnetic fields (RF-EMF) has increased public concern about its health effects. Of particular concern are the influences of RF-EMF exposure on the development of the brain. The mechanisms of how RF-EMF acts on the developing brain are not fully understood. Here, based on high-throughput RNA sequencing techniques, we revealed that transcripts related to neurite development were significantly influenced by 1800 MHz RF-EMF exposure during neuronal differentiation. Exposure to RF-EMF remarkably decreased the total length of neurite and the number of branch points in neural stem cells-derived neurons and retinoic acid-induced Neuro-2A cells. The expression of Eph receptors 5 (EPHA5), which is required for neurite outgrowth, was inhibited remarkably after RF-EMF exposure. Enhancing EPHA5 signaling rescued the inhibitory effects of RF-EMF on neurite outgrowth. Besides, we identified that cAMP-response element-binding protein (CREB) and RhoA were critical downstream factors of EPHA5 signaling in mediating the inhibitory effects of RF-EMF on neurite outgrowth. Together, our finding revealed that RF-EMF exposure impaired neurite outgrowth through EPHA5 signaling. This finding explored the effects and key mechanisms of how RF-EMF exposure impaired neurite outgrowth and also provided a new clue to understanding the influences of RF-EMF on brain development.
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spelling pubmed-80750582021-04-27 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling Chen, Chunhai Ma, Qinglong Deng, Ping Lin, Min Gao, Peng He, Mindi Lu, Yonghui Pi, Huifeng He, Zhixin Zhou, Chao Zhang, Yanwen Yu, Zhengping Zhang, Lei Front Cell Dev Biol Cell and Developmental Biology The increasing intensity of environmental radiofrequency electromagnetic fields (RF-EMF) has increased public concern about its health effects. Of particular concern are the influences of RF-EMF exposure on the development of the brain. The mechanisms of how RF-EMF acts on the developing brain are not fully understood. Here, based on high-throughput RNA sequencing techniques, we revealed that transcripts related to neurite development were significantly influenced by 1800 MHz RF-EMF exposure during neuronal differentiation. Exposure to RF-EMF remarkably decreased the total length of neurite and the number of branch points in neural stem cells-derived neurons and retinoic acid-induced Neuro-2A cells. The expression of Eph receptors 5 (EPHA5), which is required for neurite outgrowth, was inhibited remarkably after RF-EMF exposure. Enhancing EPHA5 signaling rescued the inhibitory effects of RF-EMF on neurite outgrowth. Besides, we identified that cAMP-response element-binding protein (CREB) and RhoA were critical downstream factors of EPHA5 signaling in mediating the inhibitory effects of RF-EMF on neurite outgrowth. Together, our finding revealed that RF-EMF exposure impaired neurite outgrowth through EPHA5 signaling. This finding explored the effects and key mechanisms of how RF-EMF exposure impaired neurite outgrowth and also provided a new clue to understanding the influences of RF-EMF on brain development. Frontiers Media S.A. 2021-04-12 /pmc/articles/PMC8075058/ /pubmed/33912567 http://dx.doi.org/10.3389/fcell.2021.657623 Text en Copyright © 2021 Chen, Ma, Deng, Lin, Gao, He, Lu, Pi, He, Zhou, Zhang, Yu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Chen, Chunhai
Ma, Qinglong
Deng, Ping
Lin, Min
Gao, Peng
He, Mindi
Lu, Yonghui
Pi, Huifeng
He, Zhixin
Zhou, Chao
Zhang, Yanwen
Yu, Zhengping
Zhang, Lei
1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling
title 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling
title_full 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling
title_fullStr 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling
title_full_unstemmed 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling
title_short 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling
title_sort 1800 mhz radiofrequency electromagnetic field impairs neurite outgrowth through inhibiting epha5 signaling
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075058/
https://www.ncbi.nlm.nih.gov/pubmed/33912567
http://dx.doi.org/10.3389/fcell.2021.657623
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