Cargando…
Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections
The plasmid-mediated colistin-resistance gene named mcr-1 has been recently described in different countries and it became a public health challenge. Of note, few studies have addressed the spread of Escherichia coli harboring the mcr-1 gene in both, community and hospital settings. A total of seven...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto de Medicina Tropical
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075621/ https://www.ncbi.nlm.nih.gov/pubmed/33909852 http://dx.doi.org/10.1590/S1678-9946202163038 |
_version_ | 1783684551996866560 |
---|---|
author | Paiva, Yrving Nagano, Debora Satie Cotia, Andre Luis Franco Guimarães, Thais Martins, Roberta Cristina Ruedas Perdigão, Lauro Vieira Côrtes, Marina Farrel Marchi, Ana Paula Corscadden, Louise Machado, Anna Silva de Paula, Alexandre Inácio Franco, Lucas Augusto Moyses Neves, Patricia Regina Levin, Anna Sara Costa, Silvia Figueiredo |
author_facet | Paiva, Yrving Nagano, Debora Satie Cotia, Andre Luis Franco Guimarães, Thais Martins, Roberta Cristina Ruedas Perdigão, Lauro Vieira Côrtes, Marina Farrel Marchi, Ana Paula Corscadden, Louise Machado, Anna Silva de Paula, Alexandre Inácio Franco, Lucas Augusto Moyses Neves, Patricia Regina Levin, Anna Sara Costa, Silvia Figueiredo |
author_sort | Paiva, Yrving |
collection | PubMed |
description | The plasmid-mediated colistin-resistance gene named mcr-1 has been recently described in different countries and it became a public health challenge. Of note, few studies have addressed the spread of Escherichia coli harboring the mcr-1 gene in both, community and hospital settings. A total of seven colistin-resistant E. coli carrying mcr-1, collected from 2016 to 2018, from community (n=4), healthcare-acquired infections (n=2) and colonization (n=1) were identified in three high complexity hospitals in Sao Paulo, Brazil. These colistin-resistant isolates were screened for mcr genes by PCR and all strains were submitted to Whole Genome Sequencing and the conjugation experiment. The seven strains belonged to seven distinct sequence types (ST744, ST131, ST69, ST48, ST354, ST57, ST10), and they differ regarding the resistance profiles. Transference of mcr-1 by conjugation to E. coli strain C600 was possible in five of the seven isolates. The mcr-1 gene was found in plasmid types IncX4 or IncI2. Three of the isolates have ESBL-encoding genes (bla (CTX-M-2), n=2; bla (CTX-M-8), n=1). We hereby report genetically distinct E. coli isolates, belonging to seven STs, harboring the mcr-1 gene, associated to community and healthcare-acquired infections, and colonization in patients from three hospitals in Sao Paulo. These findings point out for the potential spread of plasmid-mediated colistin-resistance mechanism in E. coli strains in Brazil. |
format | Online Article Text |
id | pubmed-8075621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-80756212021-05-13 Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections Paiva, Yrving Nagano, Debora Satie Cotia, Andre Luis Franco Guimarães, Thais Martins, Roberta Cristina Ruedas Perdigão, Lauro Vieira Côrtes, Marina Farrel Marchi, Ana Paula Corscadden, Louise Machado, Anna Silva de Paula, Alexandre Inácio Franco, Lucas Augusto Moyses Neves, Patricia Regina Levin, Anna Sara Costa, Silvia Figueiredo Rev Inst Med Trop Sao Paulo Brief Communication The plasmid-mediated colistin-resistance gene named mcr-1 has been recently described in different countries and it became a public health challenge. Of note, few studies have addressed the spread of Escherichia coli harboring the mcr-1 gene in both, community and hospital settings. A total of seven colistin-resistant E. coli carrying mcr-1, collected from 2016 to 2018, from community (n=4), healthcare-acquired infections (n=2) and colonization (n=1) were identified in three high complexity hospitals in Sao Paulo, Brazil. These colistin-resistant isolates were screened for mcr genes by PCR and all strains were submitted to Whole Genome Sequencing and the conjugation experiment. The seven strains belonged to seven distinct sequence types (ST744, ST131, ST69, ST48, ST354, ST57, ST10), and they differ regarding the resistance profiles. Transference of mcr-1 by conjugation to E. coli strain C600 was possible in five of the seven isolates. The mcr-1 gene was found in plasmid types IncX4 or IncI2. Three of the isolates have ESBL-encoding genes (bla (CTX-M-2), n=2; bla (CTX-M-8), n=1). We hereby report genetically distinct E. coli isolates, belonging to seven STs, harboring the mcr-1 gene, associated to community and healthcare-acquired infections, and colonization in patients from three hospitals in Sao Paulo. These findings point out for the potential spread of plasmid-mediated colistin-resistance mechanism in E. coli strains in Brazil. Instituto de Medicina Tropical 2021-04-23 /pmc/articles/PMC8075621/ /pubmed/33909852 http://dx.doi.org/10.1590/S1678-9946202163038 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Communication Paiva, Yrving Nagano, Debora Satie Cotia, Andre Luis Franco Guimarães, Thais Martins, Roberta Cristina Ruedas Perdigão, Lauro Vieira Côrtes, Marina Farrel Marchi, Ana Paula Corscadden, Louise Machado, Anna Silva de Paula, Alexandre Inácio Franco, Lucas Augusto Moyses Neves, Patricia Regina Levin, Anna Sara Costa, Silvia Figueiredo Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections |
title | Colistin-resistant Escherichia coli belonging to
different sequence types: genetic characterization of isolates responsible for
colonization, community- and healthcare-acquired infections |
title_full | Colistin-resistant Escherichia coli belonging to
different sequence types: genetic characterization of isolates responsible for
colonization, community- and healthcare-acquired infections |
title_fullStr | Colistin-resistant Escherichia coli belonging to
different sequence types: genetic characterization of isolates responsible for
colonization, community- and healthcare-acquired infections |
title_full_unstemmed | Colistin-resistant Escherichia coli belonging to
different sequence types: genetic characterization of isolates responsible for
colonization, community- and healthcare-acquired infections |
title_short | Colistin-resistant Escherichia coli belonging to
different sequence types: genetic characterization of isolates responsible for
colonization, community- and healthcare-acquired infections |
title_sort | colistin-resistant escherichia coli belonging to
different sequence types: genetic characterization of isolates responsible for
colonization, community- and healthcare-acquired infections |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075621/ https://www.ncbi.nlm.nih.gov/pubmed/33909852 http://dx.doi.org/10.1590/S1678-9946202163038 |
work_keys_str_mv | AT paivayrving colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT naganodeborasatie colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT cotiaandreluisfranco colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT guimaraesthais colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT martinsrobertacristinaruedas colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT perdigaolaurovieira colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT cortesmarinafarrel colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT marchianapaula colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT corscaddenlouise colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT machadoannasilva colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT depaulaalexandreinacio colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT francolucasaugustomoyses colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT nevespatriciaregina colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT levinannasara colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections AT costasilviafigueiredo colistinresistantescherichiacolibelongingtodifferentsequencetypesgeneticcharacterizationofisolatesresponsibleforcolonizationcommunityandhealthcareacquiredinfections |