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Three-dimensional live imaging of bovine embryos by optical coherence tomography

While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captur...

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Autores principales: MASUDA, Yasumitsu, HASEBE, Ryo, KUROMI, Yasushi, KOBAYASHI, Masayoshi, IWAMOTO, Misaki, HISHINUMA, Mitsugu, OHBAYASHI, Tetsuya, NISHIMURA, Ryo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075722/
https://www.ncbi.nlm.nih.gov/pubmed/33487605
http://dx.doi.org/10.1262/jrd.2020-151
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author MASUDA, Yasumitsu
HASEBE, Ryo
KUROMI, Yasushi
KOBAYASHI, Masayoshi
IWAMOTO, Misaki
HISHINUMA, Mitsugu
OHBAYASHI, Tetsuya
NISHIMURA, Ryo
author_facet MASUDA, Yasumitsu
HASEBE, Ryo
KUROMI, Yasushi
KOBAYASHI, Masayoshi
IWAMOTO, Misaki
HISHINUMA, Mitsugu
OHBAYASHI, Tetsuya
NISHIMURA, Ryo
author_sort MASUDA, Yasumitsu
collection PubMed
description While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captured by optical coherence tomography (OCT). Seven Japanese Black embryos produced by in vitro fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes of blastocoel and whole embryo, were quantified from 3D images. Four of the seven recipients became pregnant. We suggest that these 22 parameters can be potentially employed to evaluate the quality of bovine embryos before ET.
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spelling pubmed-80757222021-04-30 Three-dimensional live imaging of bovine embryos by optical coherence tomography MASUDA, Yasumitsu HASEBE, Ryo KUROMI, Yasushi KOBAYASHI, Masayoshi IWAMOTO, Misaki HISHINUMA, Mitsugu OHBAYASHI, Tetsuya NISHIMURA, Ryo J Reprod Dev Technology Report While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captured by optical coherence tomography (OCT). Seven Japanese Black embryos produced by in vitro fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes of blastocoel and whole embryo, were quantified from 3D images. Four of the seven recipients became pregnant. We suggest that these 22 parameters can be potentially employed to evaluate the quality of bovine embryos before ET. The Society for Reproduction and Development 2021-01-24 2021-04 /pmc/articles/PMC8075722/ /pubmed/33487605 http://dx.doi.org/10.1262/jrd.2020-151 Text en ©2021 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Technology Report
MASUDA, Yasumitsu
HASEBE, Ryo
KUROMI, Yasushi
KOBAYASHI, Masayoshi
IWAMOTO, Misaki
HISHINUMA, Mitsugu
OHBAYASHI, Tetsuya
NISHIMURA, Ryo
Three-dimensional live imaging of bovine embryos by optical coherence tomography
title Three-dimensional live imaging of bovine embryos by optical coherence tomography
title_full Three-dimensional live imaging of bovine embryos by optical coherence tomography
title_fullStr Three-dimensional live imaging of bovine embryos by optical coherence tomography
title_full_unstemmed Three-dimensional live imaging of bovine embryos by optical coherence tomography
title_short Three-dimensional live imaging of bovine embryos by optical coherence tomography
title_sort three-dimensional live imaging of bovine embryos by optical coherence tomography
topic Technology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075722/
https://www.ncbi.nlm.nih.gov/pubmed/33487605
http://dx.doi.org/10.1262/jrd.2020-151
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