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Three-dimensional live imaging of bovine embryos by optical coherence tomography
While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captur...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075722/ https://www.ncbi.nlm.nih.gov/pubmed/33487605 http://dx.doi.org/10.1262/jrd.2020-151 |
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author | MASUDA, Yasumitsu HASEBE, Ryo KUROMI, Yasushi KOBAYASHI, Masayoshi IWAMOTO, Misaki HISHINUMA, Mitsugu OHBAYASHI, Tetsuya NISHIMURA, Ryo |
author_facet | MASUDA, Yasumitsu HASEBE, Ryo KUROMI, Yasushi KOBAYASHI, Masayoshi IWAMOTO, Misaki HISHINUMA, Mitsugu OHBAYASHI, Tetsuya NISHIMURA, Ryo |
author_sort | MASUDA, Yasumitsu |
collection | PubMed |
description | While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captured by optical coherence tomography (OCT). Seven Japanese Black embryos produced by in vitro fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes of blastocoel and whole embryo, were quantified from 3D images. Four of the seven recipients became pregnant. We suggest that these 22 parameters can be potentially employed to evaluate the quality of bovine embryos before ET. |
format | Online Article Text |
id | pubmed-8075722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-80757222021-04-30 Three-dimensional live imaging of bovine embryos by optical coherence tomography MASUDA, Yasumitsu HASEBE, Ryo KUROMI, Yasushi KOBAYASHI, Masayoshi IWAMOTO, Misaki HISHINUMA, Mitsugu OHBAYASHI, Tetsuya NISHIMURA, Ryo J Reprod Dev Technology Report While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captured by optical coherence tomography (OCT). Seven Japanese Black embryos produced by in vitro fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes of blastocoel and whole embryo, were quantified from 3D images. Four of the seven recipients became pregnant. We suggest that these 22 parameters can be potentially employed to evaluate the quality of bovine embryos before ET. The Society for Reproduction and Development 2021-01-24 2021-04 /pmc/articles/PMC8075722/ /pubmed/33487605 http://dx.doi.org/10.1262/jrd.2020-151 Text en ©2021 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Technology Report MASUDA, Yasumitsu HASEBE, Ryo KUROMI, Yasushi KOBAYASHI, Masayoshi IWAMOTO, Misaki HISHINUMA, Mitsugu OHBAYASHI, Tetsuya NISHIMURA, Ryo Three-dimensional live imaging of bovine embryos by optical coherence tomography |
title | Three-dimensional live imaging of bovine embryos by optical coherence tomography |
title_full | Three-dimensional live imaging of bovine embryos by optical coherence tomography |
title_fullStr | Three-dimensional live imaging of bovine embryos by optical coherence tomography |
title_full_unstemmed | Three-dimensional live imaging of bovine embryos by optical coherence tomography |
title_short | Three-dimensional live imaging of bovine embryos by optical coherence tomography |
title_sort | three-dimensional live imaging of bovine embryos by optical coherence tomography |
topic | Technology Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075722/ https://www.ncbi.nlm.nih.gov/pubmed/33487605 http://dx.doi.org/10.1262/jrd.2020-151 |
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