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Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension

Obstructive sleep apnea–hypopnea syndrome (OSAHS) is an independent risk factor for hypertension (HTN). The oral microbiota plays a pathophysiological role in cardiovascular diseases; however, there are few reports directly investigating and identifying the organisms involved in OSAHS-related HTN. T...

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Autores principales: Ko, Chih-Yuan, Hu, An-Ke, Chou, Dylan, Huang, Li-Mei, Su, Huan-Zhang, Yan, Fu-Rong, Zhang, Xiao-Bin, Zhang, Hua-Ping, Zeng, Yi-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075895/
https://www.ncbi.nlm.nih.gov/pubmed/30976074
http://dx.doi.org/10.1038/s41440-019-0260-4
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author Ko, Chih-Yuan
Hu, An-Ke
Chou, Dylan
Huang, Li-Mei
Su, Huan-Zhang
Yan, Fu-Rong
Zhang, Xiao-Bin
Zhang, Hua-Ping
Zeng, Yi-Ming
author_facet Ko, Chih-Yuan
Hu, An-Ke
Chou, Dylan
Huang, Li-Mei
Su, Huan-Zhang
Yan, Fu-Rong
Zhang, Xiao-Bin
Zhang, Hua-Ping
Zeng, Yi-Ming
author_sort Ko, Chih-Yuan
collection PubMed
description Obstructive sleep apnea–hypopnea syndrome (OSAHS) is an independent risk factor for hypertension (HTN). The oral microbiota plays a pathophysiological role in cardiovascular diseases; however, there are few reports directly investigating and identifying the organisms involved in OSAHS-related HTN. Therefore, this study aimed to identify those organisms. We obtained 139 oral samples and determined the microbiome composition using pyrosequencing and bioinformatic analyses of the 16S rRNA. We examined the fasting levels of cytokines and homocysteine in all participants and analyzed the correlations between the oral microbiota and homocysteine levels. We determined the molecular mechanism underlying HTN by investigating the genetic composition of the strains in the blood. We detected higher relative abundances of Porphyromonas and Aggregatibacter and elevated proinflammatory cytokines in patients with OSAHS of varying severity compared with individuals without OSAHS; however, the two organisms were not measured in the blood samples from all participants. High levels of specific Porphyromonas bacteria were detected in patients with OSAHS with and without HTN, whereas the relative abundance of Aggregatibacter was negatively correlated with the homocysteine level. The receiver operating characteristic curve analysis of controls and patients with OSAHS resulted in area under the curve values of 0.759 and 0.641 for patients with OSAHS with or without HTN, respectively. We found that the predictive function of oral microbiota was different in patients with OSAHS with and without HTN. However, there was no direct invasion by the two organisms causing endothelial cell injury, leading to speculation regarding the other mechanisms that may lead to HTN. Elucidating the differences in the oral microbiome will help us understand the pathogenesis of OSAHS-related HTN.
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spelling pubmed-80758952021-05-06 Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension Ko, Chih-Yuan Hu, An-Ke Chou, Dylan Huang, Li-Mei Su, Huan-Zhang Yan, Fu-Rong Zhang, Xiao-Bin Zhang, Hua-Ping Zeng, Yi-Ming Hypertens Res Article Obstructive sleep apnea–hypopnea syndrome (OSAHS) is an independent risk factor for hypertension (HTN). The oral microbiota plays a pathophysiological role in cardiovascular diseases; however, there are few reports directly investigating and identifying the organisms involved in OSAHS-related HTN. Therefore, this study aimed to identify those organisms. We obtained 139 oral samples and determined the microbiome composition using pyrosequencing and bioinformatic analyses of the 16S rRNA. We examined the fasting levels of cytokines and homocysteine in all participants and analyzed the correlations between the oral microbiota and homocysteine levels. We determined the molecular mechanism underlying HTN by investigating the genetic composition of the strains in the blood. We detected higher relative abundances of Porphyromonas and Aggregatibacter and elevated proinflammatory cytokines in patients with OSAHS of varying severity compared with individuals without OSAHS; however, the two organisms were not measured in the blood samples from all participants. High levels of specific Porphyromonas bacteria were detected in patients with OSAHS with and without HTN, whereas the relative abundance of Aggregatibacter was negatively correlated with the homocysteine level. The receiver operating characteristic curve analysis of controls and patients with OSAHS resulted in area under the curve values of 0.759 and 0.641 for patients with OSAHS with or without HTN, respectively. We found that the predictive function of oral microbiota was different in patients with OSAHS with and without HTN. However, there was no direct invasion by the two organisms causing endothelial cell injury, leading to speculation regarding the other mechanisms that may lead to HTN. Elucidating the differences in the oral microbiome will help us understand the pathogenesis of OSAHS-related HTN. Springer Singapore 2019-04-11 2019 /pmc/articles/PMC8075895/ /pubmed/30976074 http://dx.doi.org/10.1038/s41440-019-0260-4 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ko, Chih-Yuan
Hu, An-Ke
Chou, Dylan
Huang, Li-Mei
Su, Huan-Zhang
Yan, Fu-Rong
Zhang, Xiao-Bin
Zhang, Hua-Ping
Zeng, Yi-Ming
Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension
title Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension
title_full Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension
title_fullStr Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension
title_full_unstemmed Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension
title_short Analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension
title_sort analysis of oral microbiota in patients with obstructive sleep apnea-associated hypertension
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075895/
https://www.ncbi.nlm.nih.gov/pubmed/30976074
http://dx.doi.org/10.1038/s41440-019-0260-4
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