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Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence
In this study, we aimed to explore the beneficial properties of novel quinoline derivatives on human sperm motility and its functional competence. Nine novel quinoline derivatives were screened for their effect on motility in human spermatozoa from normozoospermic ejaculates. Compounds with impressi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076127/ https://www.ncbi.nlm.nih.gov/pubmed/33237520 http://dx.doi.org/10.1007/s43032-020-00382-5 |
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author | Kumari, Sandhya Salian, Sujith Raj Rao, Arpitha Somagond, Shilpa M. Kamble, Ravindra R. Nesaragi, Aravind Das, Jyotirekha Rajanikant, G. K. Mutalik, Srinivas Raghu, Shamprasad Varija Adiga, Satish Kumar Kalthur, Guruprasad |
author_facet | Kumari, Sandhya Salian, Sujith Raj Rao, Arpitha Somagond, Shilpa M. Kamble, Ravindra R. Nesaragi, Aravind Das, Jyotirekha Rajanikant, G. K. Mutalik, Srinivas Raghu, Shamprasad Varija Adiga, Satish Kumar Kalthur, Guruprasad |
author_sort | Kumari, Sandhya |
collection | PubMed |
description | In this study, we aimed to explore the beneficial properties of novel quinoline derivatives on human sperm motility and its functional competence. Nine novel quinoline derivatives were screened for their effect on motility in human spermatozoa from normozoospermic ejaculates. Compounds with impressive sperm motility enhancement properties were further assessed for their effect on functional competence of human spermatozoa. To determine the effect on the fertilizing ability of spermatozoa processed with quinoline derivatives and to assess developmental competence of embryos derived, in vitro fertilization (IVF) was performed using mouse model. Among the nine quinoline derivatives, 2 compounds (6MQT and 2,6DQT) exhibited significant enhancement in sperm progressive motility and survival at 24 h. Further, non-significant increase in curvilinear velocity (VCL), straight line velocity (VSL), and amplitude of lateral head displacement (ALH) was observed. Capacitation, intracellular cAMP level and tyrosine phosphorylated sperm proteins were significantly higher in 6MQT (P < 0.05) and 2,6DQT (P < 0.001) compared to control. In vitro fertilization (IVF) experiments using Swiss albino mice revealed that spermatozoa processed with 6MQT had non-significantly higher blastocyst rate and a superior blastocyst quality, while, 2,6DQT resulted in significantly lower blastocyst rate (P < 0.05) compared to control. Quinoline derivative 6MQT has significant motility enhancement property under in vitro conditions. [Figure: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43032-020-00382-5. |
format | Online Article Text |
id | pubmed-8076127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-80761272021-05-05 Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence Kumari, Sandhya Salian, Sujith Raj Rao, Arpitha Somagond, Shilpa M. Kamble, Ravindra R. Nesaragi, Aravind Das, Jyotirekha Rajanikant, G. K. Mutalik, Srinivas Raghu, Shamprasad Varija Adiga, Satish Kumar Kalthur, Guruprasad Reprod Sci Reproductive Biology: Original Article In this study, we aimed to explore the beneficial properties of novel quinoline derivatives on human sperm motility and its functional competence. Nine novel quinoline derivatives were screened for their effect on motility in human spermatozoa from normozoospermic ejaculates. Compounds with impressive sperm motility enhancement properties were further assessed for their effect on functional competence of human spermatozoa. To determine the effect on the fertilizing ability of spermatozoa processed with quinoline derivatives and to assess developmental competence of embryos derived, in vitro fertilization (IVF) was performed using mouse model. Among the nine quinoline derivatives, 2 compounds (6MQT and 2,6DQT) exhibited significant enhancement in sperm progressive motility and survival at 24 h. Further, non-significant increase in curvilinear velocity (VCL), straight line velocity (VSL), and amplitude of lateral head displacement (ALH) was observed. Capacitation, intracellular cAMP level and tyrosine phosphorylated sperm proteins were significantly higher in 6MQT (P < 0.05) and 2,6DQT (P < 0.001) compared to control. In vitro fertilization (IVF) experiments using Swiss albino mice revealed that spermatozoa processed with 6MQT had non-significantly higher blastocyst rate and a superior blastocyst quality, while, 2,6DQT resulted in significantly lower blastocyst rate (P < 0.05) compared to control. Quinoline derivative 6MQT has significant motility enhancement property under in vitro conditions. [Figure: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43032-020-00382-5. Springer International Publishing 2020-11-25 /pmc/articles/PMC8076127/ /pubmed/33237520 http://dx.doi.org/10.1007/s43032-020-00382-5 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Reproductive Biology: Original Article Kumari, Sandhya Salian, Sujith Raj Rao, Arpitha Somagond, Shilpa M. Kamble, Ravindra R. Nesaragi, Aravind Das, Jyotirekha Rajanikant, G. K. Mutalik, Srinivas Raghu, Shamprasad Varija Adiga, Satish Kumar Kalthur, Guruprasad Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence |
title | Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence |
title_full | Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence |
title_fullStr | Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence |
title_full_unstemmed | Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence |
title_short | Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence |
title_sort | quinoline derivative enhances human sperm motility and improves the functional competence |
topic | Reproductive Biology: Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076127/ https://www.ncbi.nlm.nih.gov/pubmed/33237520 http://dx.doi.org/10.1007/s43032-020-00382-5 |
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