Cargando…

Analysis and forecasting of global real time RT-PCR primers and probes for SARS-CoV-2

Rapid tests for active SARS-CoV-2 infections rely on reverse transcription polymerase chain reaction (RT-PCR). RT-PCR uses reverse transcription of RNA into complementary DNA (cDNA) and amplification of specific DNA (primer and probe) targets using polymerase chain reaction (PCR). The technology mak...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nayar, Gowri, Seabolt, Edward E., Kunitomi, Mark, Agarwal, Akshay, Beck, Kristen L., Mukherjee, Vandana, Kaufman, James H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076216/ https://www.ncbi.nlm.nih.gov/pubmed/33903676 http://dx.doi.org/10.1038/s41598-021-88532-w

Ejemplares similares

Predicting Epitope Candidates for SARS-CoV-2
por: Agarwal, Akshay, et al.
Publicado: (2022)

Semi-Supervised Pipeline for Autonomous Annotation of SARS-CoV-2 Genomes
por: Beck, Kristen L., et al.
Publicado: (2021)

Lyophilized Matrix Containing Ready-to-Use Primers and Probe Solution for Standardization of Real-Time PCR and RT-qPCR Diagnostics in Virology
por: Thirion, Laurence, et al.
Publicado: (2020)

ggMOB: Elucidation of genomic conjugative features and associated cargo genes across bacterial genera using genus-genus mobilization networks
por: Nayar, Gowri, et al.
Publicado: (2022)

Primers and probe design and precision assessment of the real time RT-PCR assay in Coxsackievirus A10 and enterovirus detection
por: Chen, Jingfang, et al.
Publicado: (2017)

RTPrimerDB: the portal for real-time PCR primers and probes
por: Lefever, Steve, et al.
Publicado: (2009)

Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR
por: Perchetti, Garrett A., et al.
Publicado: (2020)

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets
por: Liu, Xinjin, et al.
Publicado: (2020)

Development of a highly sensitive real-time one step RT-PCR combined complementary locked primer technology and conjugated minor groove binder probe
por: Hong, JiYoung, et al.
Publicado: (2011)

RTPrimerDB: the real-time PCR primer and probe database, major update 2006
por: Pattyn, Filip, et al.
Publicado: (2006)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data
por: Tokheim, Collin, et al.
Publicado: (2014)

Analytical performance comparison of four SARS-CoV-2 RT-qPCR primer-probe sets for wastewater samples
por: Zhang, Shuxin, et al.
Publicado: (2022)

Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei
por: Wang, Wei, et al.
Publicado: (2017)

Development of a novel real-time RT-PCR assay with LUX primer for the detection of swine transmissible gastroenteritis virus
por: Chen, Ru, et al.
Publicado: (2004)

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?
por: Anantharajah, Ahalieyah, et al.
Publicado: (2021)

RT-qPCR Testing of SARS-CoV-2: A Primer
por: Bustin, Stephen A., et al.
Publicado: (2020)

A plastome primer set for comprehensive quantitative real time RT-PCR analysis of Zea mays: a starter primer set for other Poaceae species
por: Sharpe, Richard M, et al.
Publicado: (2008)

qPrimerDepot: a primer database for quantitative real time PCR
por: Cui, Wenwu, et al.
Publicado: (2007)

A tool for design of primers for microRNA-specific quantitative RT-qPCR
por: Busk, Peter K
Publicado: (2014)

Development of Multiplex RT-PCR with Immobilized Primers for Identification of Infectious Human Pneumonia Pathogens
por: Lapa, S. A., et al.
Publicado: (2021)

Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR
por: Pagliarulo, Vincenzo, et al.
Publicado: (2004)

PRISE2: Software for designing sequence-selective PCR primers and probes
por: Huang, Yu-Ting, et al.
Publicado: (2014)

Virus detection and identification using random multiplex (RT)-PCR with 3'-locked random primers
por: Clem, Amy L, et al.
Publicado: (2007)

AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs
por: Han, Sangjo, et al.
Publicado: (2006)

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells
por: Chen, Gang, et al.
Publicado: (2010)

Real-Time RT-PCR for the Detection of Lyssavirus Species
por: Deubelbeiss, A., et al.
Publicado: (2014)

Real-Time RT-PCR Detection of Equine Coronavirus
por: Miszczak, Fabien, et al.
Publicado: (2015)

SARS-CoV-2 wastewater surveillance in Germany: Long-term RT-digital droplet PCR monitoring, suitability of primer/probe combinations and biomarker stability
por: Ho, Johannes, et al.
Publicado: (2022)

Stealth Omicron: A Novel SARS-CoV-2 Variant That Is Insensitive to RT-qPCR Using the N1 and N2 Primer-Probes
por: Mori, Hideo, et al.
Publicado: (2023)

COVID-19 Real-Time RT-PCR: Does Positivity on Follow-up RT-PCR Always Imply Infectivity?
por: Suri, Tejas, et al.
Publicado: (2020)

Comparative Evaluation of Standard RT-PCR Assays and Commercial Real-Time RT-PCR Kits for Detection of Lassa Virus
por: Luo, Xue-Lian, et al.
Publicado: (2023)

Microdroplet Sandwich Real-Time RT-PCR for Detection of Pandemic and Seasonal Influenza Subtypes
por: Angione, Stephanie L., et al.
Publicado: (2013)

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping
por: Li, Baowei, et al.
Publicado: (2021)

Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets
por: Park, Changwoo, et al.
Publicado: (2021)

Simulate_PCR for amplicon prediction and annotation from multiplex, degenerate primers and probes
por: Gardner, Shea N, et al.
Publicado: (2014)

Detection of Mycobacterium ulcerans by real-time PCR with improved primers
por: Shinoda, Noriko, et al.
Publicado: (2016)

The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
por: Herbert, Mike, et al.
Publicado: (2011)

Protocol for High-Resolution Mapping of Splicing Products and Isoforms by RT-PCR Using Fluorescently Labeled Primers
por: Weathers, Indya, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_pa7ocjt4p2r5b9fp2p287lb91d