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Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens
The effects of respiratory inhibitors, quinone analogues and artificial substrates on the membrane-bound electron transport system of the fastidious β-proteobacterium Eikenella corrodens grown under O(2)-limited conditions were studied. NADH respiration in isolated membrane particles were partially...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076288/ https://www.ncbi.nlm.nih.gov/pubmed/33903681 http://dx.doi.org/10.1038/s41598-021-88388-0 |
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author | Jaramillo-Lanchero, Rubén D. Suarez-Alvarez, Paola Teheran-Sierra, Luis |
author_facet | Jaramillo-Lanchero, Rubén D. Suarez-Alvarez, Paola Teheran-Sierra, Luis |
author_sort | Jaramillo-Lanchero, Rubén D. |
collection | PubMed |
description | The effects of respiratory inhibitors, quinone analogues and artificial substrates on the membrane-bound electron transport system of the fastidious β-proteobacterium Eikenella corrodens grown under O(2)-limited conditions were studied. NADH respiration in isolated membrane particles were partially inhibited by rotenone, dicoumarol, quinacrine, flavone, and capsaicin. A similar response was obtained when succinate oxidation was performed in the presence of thenoyltrifluoroacetone and N,N’-dicyclohexylcarbodiimide. NADH respiration was resistant to site II inhibitors and cyanide, indicating that a percentage of the electrons transported can reach O(2) without the bc(1) complex. Succinate respiration was sensitive to myxothiazol, antimycin A and 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO). Juglone, plumbagin and menadione had higher reactivity with NADH dehydrogenase. The membrane particles showed the highest oxidase activities with ascorbate-TCHQ (tetrachlorohydroquinone), TCHQ alone, and NADH-TMPD (N,N,N’,N’-tetramethyl-p-phenylenediamine), and minor activity levels with ascorbate-DCPIP (2,6-dichloro-phenolindophenol) and NADH-DCPIP. The substrates NADH-DCPIP, NADH-TMPD and TCHQ were electron donors to cyanide-sensitive cbb' cytochrome c oxidase. The presence of dissimilatory nitrate reductase in the aerobic respiratory system of E. corrodens ATCC 23834 was demonstrated by first time. Our results indicate that complexes I and II have resistance to their classic inhibitors, that the oxidation of NADH is stimulated by juglone, plumbagin and menadione, and that sensitivity to KCN is stimulated by the substrates TCHQ, NADH-DCPIP and NADH-TMPD. |
format | Online Article Text |
id | pubmed-8076288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80762882021-04-27 Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens Jaramillo-Lanchero, Rubén D. Suarez-Alvarez, Paola Teheran-Sierra, Luis Sci Rep Article The effects of respiratory inhibitors, quinone analogues and artificial substrates on the membrane-bound electron transport system of the fastidious β-proteobacterium Eikenella corrodens grown under O(2)-limited conditions were studied. NADH respiration in isolated membrane particles were partially inhibited by rotenone, dicoumarol, quinacrine, flavone, and capsaicin. A similar response was obtained when succinate oxidation was performed in the presence of thenoyltrifluoroacetone and N,N’-dicyclohexylcarbodiimide. NADH respiration was resistant to site II inhibitors and cyanide, indicating that a percentage of the electrons transported can reach O(2) without the bc(1) complex. Succinate respiration was sensitive to myxothiazol, antimycin A and 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO). Juglone, plumbagin and menadione had higher reactivity with NADH dehydrogenase. The membrane particles showed the highest oxidase activities with ascorbate-TCHQ (tetrachlorohydroquinone), TCHQ alone, and NADH-TMPD (N,N,N’,N’-tetramethyl-p-phenylenediamine), and minor activity levels with ascorbate-DCPIP (2,6-dichloro-phenolindophenol) and NADH-DCPIP. The substrates NADH-DCPIP, NADH-TMPD and TCHQ were electron donors to cyanide-sensitive cbb' cytochrome c oxidase. The presence of dissimilatory nitrate reductase in the aerobic respiratory system of E. corrodens ATCC 23834 was demonstrated by first time. Our results indicate that complexes I and II have resistance to their classic inhibitors, that the oxidation of NADH is stimulated by juglone, plumbagin and menadione, and that sensitivity to KCN is stimulated by the substrates TCHQ, NADH-DCPIP and NADH-TMPD. Nature Publishing Group UK 2021-04-26 /pmc/articles/PMC8076288/ /pubmed/33903681 http://dx.doi.org/10.1038/s41598-021-88388-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Jaramillo-Lanchero, Rubén D. Suarez-Alvarez, Paola Teheran-Sierra, Luis Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens |
title | Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens |
title_full | Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens |
title_fullStr | Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens |
title_full_unstemmed | Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens |
title_short | Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens |
title_sort | effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of eikenella corrodens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076288/ https://www.ncbi.nlm.nih.gov/pubmed/33903681 http://dx.doi.org/10.1038/s41598-021-88388-0 |
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