Cargando…

Prevalence and molecular characterization of canine parvovirus

BACKGROUND AND AIM: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Parikshit, Kaur, Gurpreet, Chandra, Mudit, Dwivedi, P. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076476/
https://www.ncbi.nlm.nih.gov/pubmed/33935404
http://dx.doi.org/10.14202/vetworld.2021.603-606
_version_ 1783684688502587392
author Singh, Parikshit
Kaur, Gurpreet
Chandra, Mudit
Dwivedi, P. N.
author_facet Singh, Parikshit
Kaur, Gurpreet
Chandra, Mudit
Dwivedi, P. N.
author_sort Singh, Parikshit
collection PubMed
description BACKGROUND AND AIM: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India. MATERIALS AND METHODS: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of VP2 gene. RESULTS: The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of VP2 gene from these isolates revealed that most samples formed a clade with CPV-2a isolates. CONCLUSION: Sequence analysis and phylogenetic analysis of VP2 gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type.
format Online
Article
Text
id pubmed-8076476
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-80764762021-04-30 Prevalence and molecular characterization of canine parvovirus Singh, Parikshit Kaur, Gurpreet Chandra, Mudit Dwivedi, P. N. Vet World Research Article BACKGROUND AND AIM: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India. MATERIALS AND METHODS: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of VP2 gene. RESULTS: The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of VP2 gene from these isolates revealed that most samples formed a clade with CPV-2a isolates. CONCLUSION: Sequence analysis and phylogenetic analysis of VP2 gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type. Veterinary World 2021-03 2021-03-09 /pmc/articles/PMC8076476/ /pubmed/33935404 http://dx.doi.org/10.14202/vetworld.2021.603-606 Text en Copyright: © Singh, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Singh, Parikshit
Kaur, Gurpreet
Chandra, Mudit
Dwivedi, P. N.
Prevalence and molecular characterization of canine parvovirus
title Prevalence and molecular characterization of canine parvovirus
title_full Prevalence and molecular characterization of canine parvovirus
title_fullStr Prevalence and molecular characterization of canine parvovirus
title_full_unstemmed Prevalence and molecular characterization of canine parvovirus
title_short Prevalence and molecular characterization of canine parvovirus
title_sort prevalence and molecular characterization of canine parvovirus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076476/
https://www.ncbi.nlm.nih.gov/pubmed/33935404
http://dx.doi.org/10.14202/vetworld.2021.603-606
work_keys_str_mv AT singhparikshit prevalenceandmolecularcharacterizationofcanineparvovirus
AT kaurgurpreet prevalenceandmolecularcharacterizationofcanineparvovirus
AT chandramudit prevalenceandmolecularcharacterizationofcanineparvovirus
AT dwivedipn prevalenceandmolecularcharacterizationofcanineparvovirus