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Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs

Gut microbiota is recognized as a strong determinant of host physiology including fat metabolism and can transfer obesity-associated phenotypes from donors to recipients. However, the relationship between gut microbiota and intramuscular fat (IMF) is still largely unknown. Obese Jinhua pigs (JP) hav...

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Autores principales: Wu, Choufei, Lyu, Wentao, Hong, Qihua, Zhang, Xiaojun, Yang, Hua, Xiao, Yingping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076524/
https://www.ncbi.nlm.nih.gov/pubmed/33928112
http://dx.doi.org/10.3389/fnut.2021.675445
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author Wu, Choufei
Lyu, Wentao
Hong, Qihua
Zhang, Xiaojun
Yang, Hua
Xiao, Yingping
author_facet Wu, Choufei
Lyu, Wentao
Hong, Qihua
Zhang, Xiaojun
Yang, Hua
Xiao, Yingping
author_sort Wu, Choufei
collection PubMed
description Gut microbiota is recognized as a strong determinant of host physiology including fat metabolism and can transfer obesity-associated phenotypes from donors to recipients. However, the relationship between gut microbiota and intramuscular fat (IMF) is still largely unknown. Obese Jinhua pigs (JP) have better meat quality that is associated with higher IMF content than lean Landrace pigs (LP). The present study was conducted to test the contribution of gut microbiota to IMF properties by transplanting fecal microbiota of adult JP and LP to antibiotics-treated mice. Similar to JP donors, the mice receiving JP's microbiota (JM) had elevated lipid and triglyceride levels and the lipoprotein lipase activity, as well as reduced mRNA level of angiopoietin-like 4 (ANGPTL4) in the gastrocnemius muscles, compared to those in mice receiving LP's microbiota (LM). High-throughput 16S rRNA sequencing confirmed that transplantation of JP and LP feces differently reconstructed the gut microbiota in both jejunum and colon of mouse recipients. In colonic samples, we observed an elevated ratio of Firmicutes to Bacteroidetes and increased abundance of genus Romboutsia in JM, which were positively correlated with obesity. Furthermore, the abundance of Akkermansia decreased in JM, which is positively correlated with lean. Colonic concentrations of acetate (P = 0.047) and butyrate (P = 0.014) were significantly lower in JM than in LM, and consistently, the terminal genes for butyrate synthesis, butyryl CoA: acetate CoA transferase were less abundant in colonic microbiota of JM. Taken together, these gut microbiota of obese JP intrinsically promotes IMF accumulation and can transfer the properties to mouse recipients. Manipulation of intestinal microbiota will, therefore, have the potential to improve the meat quality and flavor of pigs and even to ameliorate the metabolic syndrome in human.
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spelling pubmed-80765242021-04-28 Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs Wu, Choufei Lyu, Wentao Hong, Qihua Zhang, Xiaojun Yang, Hua Xiao, Yingping Front Nutr Nutrition Gut microbiota is recognized as a strong determinant of host physiology including fat metabolism and can transfer obesity-associated phenotypes from donors to recipients. However, the relationship between gut microbiota and intramuscular fat (IMF) is still largely unknown. Obese Jinhua pigs (JP) have better meat quality that is associated with higher IMF content than lean Landrace pigs (LP). The present study was conducted to test the contribution of gut microbiota to IMF properties by transplanting fecal microbiota of adult JP and LP to antibiotics-treated mice. Similar to JP donors, the mice receiving JP's microbiota (JM) had elevated lipid and triglyceride levels and the lipoprotein lipase activity, as well as reduced mRNA level of angiopoietin-like 4 (ANGPTL4) in the gastrocnemius muscles, compared to those in mice receiving LP's microbiota (LM). High-throughput 16S rRNA sequencing confirmed that transplantation of JP and LP feces differently reconstructed the gut microbiota in both jejunum and colon of mouse recipients. In colonic samples, we observed an elevated ratio of Firmicutes to Bacteroidetes and increased abundance of genus Romboutsia in JM, which were positively correlated with obesity. Furthermore, the abundance of Akkermansia decreased in JM, which is positively correlated with lean. Colonic concentrations of acetate (P = 0.047) and butyrate (P = 0.014) were significantly lower in JM than in LM, and consistently, the terminal genes for butyrate synthesis, butyryl CoA: acetate CoA transferase were less abundant in colonic microbiota of JM. Taken together, these gut microbiota of obese JP intrinsically promotes IMF accumulation and can transfer the properties to mouse recipients. Manipulation of intestinal microbiota will, therefore, have the potential to improve the meat quality and flavor of pigs and even to ameliorate the metabolic syndrome in human. Frontiers Media S.A. 2021-04-13 /pmc/articles/PMC8076524/ /pubmed/33928112 http://dx.doi.org/10.3389/fnut.2021.675445 Text en Copyright © 2021 Wu, Lyu, Hong, Zhang, Yang and Xiao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Wu, Choufei
Lyu, Wentao
Hong, Qihua
Zhang, Xiaojun
Yang, Hua
Xiao, Yingping
Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs
title Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs
title_full Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs
title_fullStr Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs
title_full_unstemmed Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs
title_short Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs
title_sort gut microbiota influence lipid metabolism of skeletal muscle in pigs
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076524/
https://www.ncbi.nlm.nih.gov/pubmed/33928112
http://dx.doi.org/10.3389/fnut.2021.675445
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