Messenger RNA capture sequencing of extracellular RNA from human biofluids using a comprehensive set of spike-in controls

Comprehensive transcriptome analysis of extracellular RNA (exRNA) purified from human biofluids is challenging because of the low RNA concentration and compromised RNA integrity. Here, we describe an optimized workflow to (1) isolate exRNA from different types of biofluids and (2) to prepare messeng...

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Detalles Bibliográficos
Autores principales: Hulstaert, Eva, Decock, Anneleen, Morlion, Annelien, Everaert, Celine, Verniers, Kimberly, Nuytens, Justine, Nijs, Nele, Schroth, Gary P., Kuersten, Scott, Gross, Stephen M., Mestdagh, Pieter, Vandesompele, Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076706/
https://www.ncbi.nlm.nih.gov/pubmed/33937877
http://dx.doi.org/10.1016/j.xpro.2021.100475

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