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Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity

Extracellular vesicles (EVs) are emerging as key players in different stages of atherosclerosis. Here we provide evidence that EVs released by mixed aggregates of monocytes and platelets in response to TNF‐α display pro‐inflammatory actions on endothelial cells and atherosclerotic plaques. Tempering...

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Autores principales: Oggero, Silvia, de Gaetano, Monica, Marcone, Simone, Fitzsimons, Stephen, Pinto, Andreia L., Ikramova, Dinara, Barry, Mary, Burke, David, Montero‐Melendez, Trinidad, Cooper, Dianne, Burgoyne, Thomas, Belton, Orina, Norling, Lucy V., Brennan, Eoin P., Godson, Catherine, Perretti, Mauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077084/
https://www.ncbi.nlm.nih.gov/pubmed/33936566
http://dx.doi.org/10.1002/jev2.12084
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author Oggero, Silvia
de Gaetano, Monica
Marcone, Simone
Fitzsimons, Stephen
Pinto, Andreia L.
Ikramova, Dinara
Barry, Mary
Burke, David
Montero‐Melendez, Trinidad
Cooper, Dianne
Burgoyne, Thomas
Belton, Orina
Norling, Lucy V.
Brennan, Eoin P.
Godson, Catherine
Perretti, Mauro
author_facet Oggero, Silvia
de Gaetano, Monica
Marcone, Simone
Fitzsimons, Stephen
Pinto, Andreia L.
Ikramova, Dinara
Barry, Mary
Burke, David
Montero‐Melendez, Trinidad
Cooper, Dianne
Burgoyne, Thomas
Belton, Orina
Norling, Lucy V.
Brennan, Eoin P.
Godson, Catherine
Perretti, Mauro
author_sort Oggero, Silvia
collection PubMed
description Extracellular vesicles (EVs) are emerging as key players in different stages of atherosclerosis. Here we provide evidence that EVs released by mixed aggregates of monocytes and platelets in response to TNF‐α display pro‐inflammatory actions on endothelial cells and atherosclerotic plaques. Tempering platelet activation with Iloprost, Aspirin or a P2Y(12) inhibitor impacted quantity and phenotype of EV produced. Proteomics of EVs from cells activated with TNF‐α alone or in the presence of Iloprost revealed a distinct composition, with interesting hits like annexin‐A1 and gelsolin. When added to human atherosclerotic plaque explants, EVs from TNF‐α stimulated monocytes augmented release of cytokines. In contrast, EVs generated by TNF‐α together with Iloprost produced minimal plaque activation. Notably, patients with coronary artery disease that required percutaneous coronary intervention had elevated plasma numbers of monocyte, platelet as well as double positive EV subsets. In conclusion, EVs released following monocyte/platelet activation may play a potential role in the development and progression of atherosclerosis. Whereas attenuating platelet activation modifies EV composition released from monocyte/platelet aggregates, curbing their pro‐inflammatory actions may offer therapeutic avenues for the treatment of atherosclerosis.
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spelling pubmed-80770842021-04-29 Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity Oggero, Silvia de Gaetano, Monica Marcone, Simone Fitzsimons, Stephen Pinto, Andreia L. Ikramova, Dinara Barry, Mary Burke, David Montero‐Melendez, Trinidad Cooper, Dianne Burgoyne, Thomas Belton, Orina Norling, Lucy V. Brennan, Eoin P. Godson, Catherine Perretti, Mauro J Extracell Vesicles Research Articles Extracellular vesicles (EVs) are emerging as key players in different stages of atherosclerosis. Here we provide evidence that EVs released by mixed aggregates of monocytes and platelets in response to TNF‐α display pro‐inflammatory actions on endothelial cells and atherosclerotic plaques. Tempering platelet activation with Iloprost, Aspirin or a P2Y(12) inhibitor impacted quantity and phenotype of EV produced. Proteomics of EVs from cells activated with TNF‐α alone or in the presence of Iloprost revealed a distinct composition, with interesting hits like annexin‐A1 and gelsolin. When added to human atherosclerotic plaque explants, EVs from TNF‐α stimulated monocytes augmented release of cytokines. In contrast, EVs generated by TNF‐α together with Iloprost produced minimal plaque activation. Notably, patients with coronary artery disease that required percutaneous coronary intervention had elevated plasma numbers of monocyte, platelet as well as double positive EV subsets. In conclusion, EVs released following monocyte/platelet activation may play a potential role in the development and progression of atherosclerosis. Whereas attenuating platelet activation modifies EV composition released from monocyte/platelet aggregates, curbing their pro‐inflammatory actions may offer therapeutic avenues for the treatment of atherosclerosis. John Wiley and Sons Inc. 2021-04-27 2021-04 /pmc/articles/PMC8077084/ /pubmed/33936566 http://dx.doi.org/10.1002/jev2.12084 Text en © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Oggero, Silvia
de Gaetano, Monica
Marcone, Simone
Fitzsimons, Stephen
Pinto, Andreia L.
Ikramova, Dinara
Barry, Mary
Burke, David
Montero‐Melendez, Trinidad
Cooper, Dianne
Burgoyne, Thomas
Belton, Orina
Norling, Lucy V.
Brennan, Eoin P.
Godson, Catherine
Perretti, Mauro
Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
title Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
title_full Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
title_fullStr Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
title_full_unstemmed Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
title_short Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
title_sort extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077084/
https://www.ncbi.nlm.nih.gov/pubmed/33936566
http://dx.doi.org/10.1002/jev2.12084
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