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Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity
Extracellular vesicles (EVs) are emerging as key players in different stages of atherosclerosis. Here we provide evidence that EVs released by mixed aggregates of monocytes and platelets in response to TNF‐α display pro‐inflammatory actions on endothelial cells and atherosclerotic plaques. Tempering...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077084/ https://www.ncbi.nlm.nih.gov/pubmed/33936566 http://dx.doi.org/10.1002/jev2.12084 |
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author | Oggero, Silvia de Gaetano, Monica Marcone, Simone Fitzsimons, Stephen Pinto, Andreia L. Ikramova, Dinara Barry, Mary Burke, David Montero‐Melendez, Trinidad Cooper, Dianne Burgoyne, Thomas Belton, Orina Norling, Lucy V. Brennan, Eoin P. Godson, Catherine Perretti, Mauro |
author_facet | Oggero, Silvia de Gaetano, Monica Marcone, Simone Fitzsimons, Stephen Pinto, Andreia L. Ikramova, Dinara Barry, Mary Burke, David Montero‐Melendez, Trinidad Cooper, Dianne Burgoyne, Thomas Belton, Orina Norling, Lucy V. Brennan, Eoin P. Godson, Catherine Perretti, Mauro |
author_sort | Oggero, Silvia |
collection | PubMed |
description | Extracellular vesicles (EVs) are emerging as key players in different stages of atherosclerosis. Here we provide evidence that EVs released by mixed aggregates of monocytes and platelets in response to TNF‐α display pro‐inflammatory actions on endothelial cells and atherosclerotic plaques. Tempering platelet activation with Iloprost, Aspirin or a P2Y(12) inhibitor impacted quantity and phenotype of EV produced. Proteomics of EVs from cells activated with TNF‐α alone or in the presence of Iloprost revealed a distinct composition, with interesting hits like annexin‐A1 and gelsolin. When added to human atherosclerotic plaque explants, EVs from TNF‐α stimulated monocytes augmented release of cytokines. In contrast, EVs generated by TNF‐α together with Iloprost produced minimal plaque activation. Notably, patients with coronary artery disease that required percutaneous coronary intervention had elevated plasma numbers of monocyte, platelet as well as double positive EV subsets. In conclusion, EVs released following monocyte/platelet activation may play a potential role in the development and progression of atherosclerosis. Whereas attenuating platelet activation modifies EV composition released from monocyte/platelet aggregates, curbing their pro‐inflammatory actions may offer therapeutic avenues for the treatment of atherosclerosis. |
format | Online Article Text |
id | pubmed-8077084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80770842021-04-29 Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity Oggero, Silvia de Gaetano, Monica Marcone, Simone Fitzsimons, Stephen Pinto, Andreia L. Ikramova, Dinara Barry, Mary Burke, David Montero‐Melendez, Trinidad Cooper, Dianne Burgoyne, Thomas Belton, Orina Norling, Lucy V. Brennan, Eoin P. Godson, Catherine Perretti, Mauro J Extracell Vesicles Research Articles Extracellular vesicles (EVs) are emerging as key players in different stages of atherosclerosis. Here we provide evidence that EVs released by mixed aggregates of monocytes and platelets in response to TNF‐α display pro‐inflammatory actions on endothelial cells and atherosclerotic plaques. Tempering platelet activation with Iloprost, Aspirin or a P2Y(12) inhibitor impacted quantity and phenotype of EV produced. Proteomics of EVs from cells activated with TNF‐α alone or in the presence of Iloprost revealed a distinct composition, with interesting hits like annexin‐A1 and gelsolin. When added to human atherosclerotic plaque explants, EVs from TNF‐α stimulated monocytes augmented release of cytokines. In contrast, EVs generated by TNF‐α together with Iloprost produced minimal plaque activation. Notably, patients with coronary artery disease that required percutaneous coronary intervention had elevated plasma numbers of monocyte, platelet as well as double positive EV subsets. In conclusion, EVs released following monocyte/platelet activation may play a potential role in the development and progression of atherosclerosis. Whereas attenuating platelet activation modifies EV composition released from monocyte/platelet aggregates, curbing their pro‐inflammatory actions may offer therapeutic avenues for the treatment of atherosclerosis. John Wiley and Sons Inc. 2021-04-27 2021-04 /pmc/articles/PMC8077084/ /pubmed/33936566 http://dx.doi.org/10.1002/jev2.12084 Text en © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Oggero, Silvia de Gaetano, Monica Marcone, Simone Fitzsimons, Stephen Pinto, Andreia L. Ikramova, Dinara Barry, Mary Burke, David Montero‐Melendez, Trinidad Cooper, Dianne Burgoyne, Thomas Belton, Orina Norling, Lucy V. Brennan, Eoin P. Godson, Catherine Perretti, Mauro Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity |
title | Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity |
title_full | Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity |
title_fullStr | Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity |
title_full_unstemmed | Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity |
title_short | Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity |
title_sort | extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077084/ https://www.ncbi.nlm.nih.gov/pubmed/33936566 http://dx.doi.org/10.1002/jev2.12084 |
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