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Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells

Chelidonium majus has been used as a traditional medicine in China and western countries for various diseases, including inflammation and cancer. However, the anti-cancer effect of chelidonine, a major compound of C. majus extracts, on pancreatic cancer remains poorly understood. In this study, we f...

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Autores principales: Jang, Hyun-Jin, Yang, Jae Ho, Hong, Eunmi, Jo, Eunbi, Lee, Soon, Lee, Sanghun, Choi, Jong Soon, Yoo, Hwa Seung, Kang, Hyuno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077490/
https://www.ncbi.nlm.nih.gov/pubmed/33884928
http://dx.doi.org/10.1177/15347354211006191
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author Jang, Hyun-Jin
Yang, Jae Ho
Hong, Eunmi
Jo, Eunbi
Lee, Soon
Lee, Sanghun
Choi, Jong Soon
Yoo, Hwa Seung
Kang, Hyuno
author_facet Jang, Hyun-Jin
Yang, Jae Ho
Hong, Eunmi
Jo, Eunbi
Lee, Soon
Lee, Sanghun
Choi, Jong Soon
Yoo, Hwa Seung
Kang, Hyuno
author_sort Jang, Hyun-Jin
collection PubMed
description Chelidonium majus has been used as a traditional medicine in China and western countries for various diseases, including inflammation and cancer. However, the anti-cancer effect of chelidonine, a major compound of C. majus extracts, on pancreatic cancer remains poorly understood. In this study, we found that treatment with chelidonine inhibited proliferation of BxPC-3 and MIA PaCa-2 human pancreatic cancer cells. Annexin-V/propidium iodide staining assay showed that this growth inhibitory effect of chelidonine was induced through apoptosis. We found that chelidonine treatment upregulated mRNA levels and transcription factor activity in both cell lines. Increases in protein expression levels of p53, GADD45A, p21 and cleaved caspase-3 were also observed, with more distinct changes in MIA PaCa-2 cells compared to the BxPC-3 cells. These results suggest that chelidonine induces pancreatic cancer apoptosis through the p53 and GADD45A pathways. Our findings provide new insights into the use of chelidonine for the treatment of pancreatic cancer.
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spelling pubmed-80774902021-05-13 Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells Jang, Hyun-Jin Yang, Jae Ho Hong, Eunmi Jo, Eunbi Lee, Soon Lee, Sanghun Choi, Jong Soon Yoo, Hwa Seung Kang, Hyuno Integr Cancer Ther Research Articles Chelidonium majus has been used as a traditional medicine in China and western countries for various diseases, including inflammation and cancer. However, the anti-cancer effect of chelidonine, a major compound of C. majus extracts, on pancreatic cancer remains poorly understood. In this study, we found that treatment with chelidonine inhibited proliferation of BxPC-3 and MIA PaCa-2 human pancreatic cancer cells. Annexin-V/propidium iodide staining assay showed that this growth inhibitory effect of chelidonine was induced through apoptosis. We found that chelidonine treatment upregulated mRNA levels and transcription factor activity in both cell lines. Increases in protein expression levels of p53, GADD45A, p21 and cleaved caspase-3 were also observed, with more distinct changes in MIA PaCa-2 cells compared to the BxPC-3 cells. These results suggest that chelidonine induces pancreatic cancer apoptosis through the p53 and GADD45A pathways. Our findings provide new insights into the use of chelidonine for the treatment of pancreatic cancer. SAGE Publications 2021-04-22 /pmc/articles/PMC8077490/ /pubmed/33884928 http://dx.doi.org/10.1177/15347354211006191 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Articles
Jang, Hyun-Jin
Yang, Jae Ho
Hong, Eunmi
Jo, Eunbi
Lee, Soon
Lee, Sanghun
Choi, Jong Soon
Yoo, Hwa Seung
Kang, Hyuno
Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells
title Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells
title_full Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells
title_fullStr Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells
title_full_unstemmed Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells
title_short Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells
title_sort chelidonine induces apoptosis via gadd45a-p53 regulation in human pancreatic cancer cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077490/
https://www.ncbi.nlm.nih.gov/pubmed/33884928
http://dx.doi.org/10.1177/15347354211006191
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