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Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells
Chelidonium majus has been used as a traditional medicine in China and western countries for various diseases, including inflammation and cancer. However, the anti-cancer effect of chelidonine, a major compound of C. majus extracts, on pancreatic cancer remains poorly understood. In this study, we f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077490/ https://www.ncbi.nlm.nih.gov/pubmed/33884928 http://dx.doi.org/10.1177/15347354211006191 |
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author | Jang, Hyun-Jin Yang, Jae Ho Hong, Eunmi Jo, Eunbi Lee, Soon Lee, Sanghun Choi, Jong Soon Yoo, Hwa Seung Kang, Hyuno |
author_facet | Jang, Hyun-Jin Yang, Jae Ho Hong, Eunmi Jo, Eunbi Lee, Soon Lee, Sanghun Choi, Jong Soon Yoo, Hwa Seung Kang, Hyuno |
author_sort | Jang, Hyun-Jin |
collection | PubMed |
description | Chelidonium majus has been used as a traditional medicine in China and western countries for various diseases, including inflammation and cancer. However, the anti-cancer effect of chelidonine, a major compound of C. majus extracts, on pancreatic cancer remains poorly understood. In this study, we found that treatment with chelidonine inhibited proliferation of BxPC-3 and MIA PaCa-2 human pancreatic cancer cells. Annexin-V/propidium iodide staining assay showed that this growth inhibitory effect of chelidonine was induced through apoptosis. We found that chelidonine treatment upregulated mRNA levels and transcription factor activity in both cell lines. Increases in protein expression levels of p53, GADD45A, p21 and cleaved caspase-3 were also observed, with more distinct changes in MIA PaCa-2 cells compared to the BxPC-3 cells. These results suggest that chelidonine induces pancreatic cancer apoptosis through the p53 and GADD45A pathways. Our findings provide new insights into the use of chelidonine for the treatment of pancreatic cancer. |
format | Online Article Text |
id | pubmed-8077490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-80774902021-05-13 Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells Jang, Hyun-Jin Yang, Jae Ho Hong, Eunmi Jo, Eunbi Lee, Soon Lee, Sanghun Choi, Jong Soon Yoo, Hwa Seung Kang, Hyuno Integr Cancer Ther Research Articles Chelidonium majus has been used as a traditional medicine in China and western countries for various diseases, including inflammation and cancer. However, the anti-cancer effect of chelidonine, a major compound of C. majus extracts, on pancreatic cancer remains poorly understood. In this study, we found that treatment with chelidonine inhibited proliferation of BxPC-3 and MIA PaCa-2 human pancreatic cancer cells. Annexin-V/propidium iodide staining assay showed that this growth inhibitory effect of chelidonine was induced through apoptosis. We found that chelidonine treatment upregulated mRNA levels and transcription factor activity in both cell lines. Increases in protein expression levels of p53, GADD45A, p21 and cleaved caspase-3 were also observed, with more distinct changes in MIA PaCa-2 cells compared to the BxPC-3 cells. These results suggest that chelidonine induces pancreatic cancer apoptosis through the p53 and GADD45A pathways. Our findings provide new insights into the use of chelidonine for the treatment of pancreatic cancer. SAGE Publications 2021-04-22 /pmc/articles/PMC8077490/ /pubmed/33884928 http://dx.doi.org/10.1177/15347354211006191 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Research Articles Jang, Hyun-Jin Yang, Jae Ho Hong, Eunmi Jo, Eunbi Lee, Soon Lee, Sanghun Choi, Jong Soon Yoo, Hwa Seung Kang, Hyuno Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells |
title | Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human
Pancreatic Cancer Cells |
title_full | Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human
Pancreatic Cancer Cells |
title_fullStr | Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human
Pancreatic Cancer Cells |
title_full_unstemmed | Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human
Pancreatic Cancer Cells |
title_short | Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human
Pancreatic Cancer Cells |
title_sort | chelidonine induces apoptosis via gadd45a-p53 regulation in human
pancreatic cancer cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077490/ https://www.ncbi.nlm.nih.gov/pubmed/33884928 http://dx.doi.org/10.1177/15347354211006191 |
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