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Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China

BACKGROUND: Staphylococcus aureus (S. aureus), especially methicillin-resistant Staphylococcus aureus (MRSA), is considered a common zoonotic pathogen, causing severe infections. The objective of this study was to investigate the antimicrobial susceptibility, resistance genes and molecular epidemiol...

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Autores principales: Gan, Ting, Shu, Gang, Fu, Hualin, Yan, Qigui, Zhang, Wei, Tang, Huaqiao, Yin, Lizi, Zhao, Ling, Lin, Juchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077920/
https://www.ncbi.nlm.nih.gov/pubmed/33902574
http://dx.doi.org/10.1186/s12917-021-02884-z
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author Gan, Ting
Shu, Gang
Fu, Hualin
Yan, Qigui
Zhang, Wei
Tang, Huaqiao
Yin, Lizi
Zhao, Ling
Lin, Juchun
author_facet Gan, Ting
Shu, Gang
Fu, Hualin
Yan, Qigui
Zhang, Wei
Tang, Huaqiao
Yin, Lizi
Zhao, Ling
Lin, Juchun
author_sort Gan, Ting
collection PubMed
description BACKGROUND: Staphylococcus aureus (S. aureus), especially methicillin-resistant Staphylococcus aureus (MRSA), is considered a common zoonotic pathogen, causing severe infections. The objective of this study was to investigate the antimicrobial susceptibility, resistance genes and molecular epidemiology among MRSA and methicillin-susceptible Staphylococcus aureus (MSSA) isolated from food animals in Sichuan Province, China. METHODS: This study was conducted on 236 S. aureus isolates. All isolates were subjected to antimicrobial susceptibility testing by using a standard microbroth dilution method. The Polymerase Chain Reaction (PCR) was performed to identify genes encoding the β-lactams resistance (blaZ, mecA), macrolides (ermA, ermB, ermC) and aminoglycosides (aacA-aphD). The molecular structures and genomic relatedness of MRSA isolates were determined by staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis (PFGE), respectively. RESULTS: Among 236 isolates, 24 (10.17 %) were recognized as MRSA. MRSA isolates showed different resistance rates to 11 antimicrobials ranging from 33.33 to 100 %, while for MSSA isolates the rates varied from 8.02 to 91.51 %. Multi-drug resistance phenotype was found in all MRSA isolates. The ermC gene encoding macrolides-lincosamides-streptogramin B was the most prevalent gene detected in 87.29 % of the S. aureus isolates, followed by ermB (83.05 %), blaZ (63.98 %), aacA-aphD (44.07 %), ermA (11.44 %) and mecA (11.02 %) genes. The prevalence of resistance genes in MRSA isolates was significantly higher than that of MSSA. Regarding the molecular morphology, SCCmec III (12/24, 50 %) was the most common SCCmec type. Furthermore, the PFGE typing showed that 24 MRSA were divided into 15 cluster groups (A to O), the major pulsotype J encompassed 25 % of MRSA isolates. CONCLUSIONS: The S. aureus isolates from food animals in Sichuan province of China have severe antimicrobials resistance with various resistance genes, especially MRSA isolates. Additionally, the genetic pool of MRSA isolates is diverse and complex, and further investigation is necessary. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-021-02884-z.
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spelling pubmed-80779202021-04-29 Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China Gan, Ting Shu, Gang Fu, Hualin Yan, Qigui Zhang, Wei Tang, Huaqiao Yin, Lizi Zhao, Ling Lin, Juchun BMC Vet Res Research BACKGROUND: Staphylococcus aureus (S. aureus), especially methicillin-resistant Staphylococcus aureus (MRSA), is considered a common zoonotic pathogen, causing severe infections. The objective of this study was to investigate the antimicrobial susceptibility, resistance genes and molecular epidemiology among MRSA and methicillin-susceptible Staphylococcus aureus (MSSA) isolated from food animals in Sichuan Province, China. METHODS: This study was conducted on 236 S. aureus isolates. All isolates were subjected to antimicrobial susceptibility testing by using a standard microbroth dilution method. The Polymerase Chain Reaction (PCR) was performed to identify genes encoding the β-lactams resistance (blaZ, mecA), macrolides (ermA, ermB, ermC) and aminoglycosides (aacA-aphD). The molecular structures and genomic relatedness of MRSA isolates were determined by staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis (PFGE), respectively. RESULTS: Among 236 isolates, 24 (10.17 %) were recognized as MRSA. MRSA isolates showed different resistance rates to 11 antimicrobials ranging from 33.33 to 100 %, while for MSSA isolates the rates varied from 8.02 to 91.51 %. Multi-drug resistance phenotype was found in all MRSA isolates. The ermC gene encoding macrolides-lincosamides-streptogramin B was the most prevalent gene detected in 87.29 % of the S. aureus isolates, followed by ermB (83.05 %), blaZ (63.98 %), aacA-aphD (44.07 %), ermA (11.44 %) and mecA (11.02 %) genes. The prevalence of resistance genes in MRSA isolates was significantly higher than that of MSSA. Regarding the molecular morphology, SCCmec III (12/24, 50 %) was the most common SCCmec type. Furthermore, the PFGE typing showed that 24 MRSA were divided into 15 cluster groups (A to O), the major pulsotype J encompassed 25 % of MRSA isolates. CONCLUSIONS: The S. aureus isolates from food animals in Sichuan province of China have severe antimicrobials resistance with various resistance genes, especially MRSA isolates. Additionally, the genetic pool of MRSA isolates is diverse and complex, and further investigation is necessary. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-021-02884-z. BioMed Central 2021-04-26 /pmc/articles/PMC8077920/ /pubmed/33902574 http://dx.doi.org/10.1186/s12917-021-02884-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Gan, Ting
Shu, Gang
Fu, Hualin
Yan, Qigui
Zhang, Wei
Tang, Huaqiao
Yin, Lizi
Zhao, Ling
Lin, Juchun
Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China
title Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China
title_full Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China
title_fullStr Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China
title_full_unstemmed Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China
title_short Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China
title_sort antimicrobial resistance and genotyping of staphylococcus aureus obtained from food animals in sichuan province, china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077920/
https://www.ncbi.nlm.nih.gov/pubmed/33902574
http://dx.doi.org/10.1186/s12917-021-02884-z
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