Cargando…

MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma

BACKGROUND: Esophageal cancer is one of the most common malignancies among Iranians and is categorized as adenocarcinoma and squamous cell carcinoma. Various environmental and genetic factors are involved in this malignancy. Despite the recent advances in therapeutic modalities there is still a noti...

Descripción completa

Detalles Bibliográficos
Autores principales: Abbaszadegan, Mohammad Reza, Taghehchian, Negin, Aarabi, Azadeh, Akbari, Faride, Saburi, Ehsan, Moghbeli, Meysam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077922/
https://www.ncbi.nlm.nih.gov/pubmed/33902648
http://dx.doi.org/10.1186/s13000-021-01098-z
_version_ 1783684976315727872
author Abbaszadegan, Mohammad Reza
Taghehchian, Negin
Aarabi, Azadeh
Akbari, Faride
Saburi, Ehsan
Moghbeli, Meysam
author_facet Abbaszadegan, Mohammad Reza
Taghehchian, Negin
Aarabi, Azadeh
Akbari, Faride
Saburi, Ehsan
Moghbeli, Meysam
author_sort Abbaszadegan, Mohammad Reza
collection PubMed
description BACKGROUND: Esophageal cancer is one of the most common malignancies among Iranians and is categorized as adenocarcinoma and squamous cell carcinoma. Various environmental and genetic factors are involved in this malignancy. Despite the recent advances in therapeutic modalities there is still a noticeable mortality rate among such patients which can be related to the late diagnosis. Regarding high ratio of esophageal squamous cell carcinoma (ESCC) in Iran, therefore it is required to assess molecular biology of ESCC to introduce novel diagnostic markers. In present study we assessed the role of Maelstrom (MAEL) cancer testis gene in biology of ESCC among Iranian patients. METHODS: Forty-five freshly normal and tumor tissues were enrolled to evaluate the levels of MAEL mRNA expression using Real time polymerase chain reaction. RESULTS: MAEL under and over expressions were observed in 12 (26.7%) and 9 (20%) of patients, respectively. MAEL fold changes were ranged between -4.33 to -1.87 (mean SD: -2.90± 0.24) and 1.92 to 7.72 (mean SD: 3.97± 0.69) in under and over expressed cases, respectively. There was a significant association between stage and MAEL expression in which majority of MAEL over expressed tumors (8/9, 88.9%) were in stage I/II (p<0.001). There was also a significant correlation between MAEL expression and depth of invasion in which tumor with T1/2 had higher levels of MAEL expression compared with T3/4 tumors (p=0.017). Moreover, there were significant correlations between MAEL expression, tumor size (p=0.028), and grade (p=0.003) among male patients. CONCLUSIONS: Our data showed that the MAEL was mainly involved in primary stages of tumor progression and it has a declining expression levels toward the advanced stages and higher depth of tumor invasions. Therefore, MAEL can be efficiently introduced as an early detection marker among Iranian ESCC patients.
format Online
Article
Text
id pubmed-8077922
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-80779222021-04-29 MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma Abbaszadegan, Mohammad Reza Taghehchian, Negin Aarabi, Azadeh Akbari, Faride Saburi, Ehsan Moghbeli, Meysam Diagn Pathol Research BACKGROUND: Esophageal cancer is one of the most common malignancies among Iranians and is categorized as adenocarcinoma and squamous cell carcinoma. Various environmental and genetic factors are involved in this malignancy. Despite the recent advances in therapeutic modalities there is still a noticeable mortality rate among such patients which can be related to the late diagnosis. Regarding high ratio of esophageal squamous cell carcinoma (ESCC) in Iran, therefore it is required to assess molecular biology of ESCC to introduce novel diagnostic markers. In present study we assessed the role of Maelstrom (MAEL) cancer testis gene in biology of ESCC among Iranian patients. METHODS: Forty-five freshly normal and tumor tissues were enrolled to evaluate the levels of MAEL mRNA expression using Real time polymerase chain reaction. RESULTS: MAEL under and over expressions were observed in 12 (26.7%) and 9 (20%) of patients, respectively. MAEL fold changes were ranged between -4.33 to -1.87 (mean SD: -2.90± 0.24) and 1.92 to 7.72 (mean SD: 3.97± 0.69) in under and over expressed cases, respectively. There was a significant association between stage and MAEL expression in which majority of MAEL over expressed tumors (8/9, 88.9%) were in stage I/II (p<0.001). There was also a significant correlation between MAEL expression and depth of invasion in which tumor with T1/2 had higher levels of MAEL expression compared with T3/4 tumors (p=0.017). Moreover, there were significant correlations between MAEL expression, tumor size (p=0.028), and grade (p=0.003) among male patients. CONCLUSIONS: Our data showed that the MAEL was mainly involved in primary stages of tumor progression and it has a declining expression levels toward the advanced stages and higher depth of tumor invasions. Therefore, MAEL can be efficiently introduced as an early detection marker among Iranian ESCC patients. BioMed Central 2021-04-26 /pmc/articles/PMC8077922/ /pubmed/33902648 http://dx.doi.org/10.1186/s13000-021-01098-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Abbaszadegan, Mohammad Reza
Taghehchian, Negin
Aarabi, Azadeh
Akbari, Faride
Saburi, Ehsan
Moghbeli, Meysam
MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma
title MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma
title_full MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma
title_fullStr MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma
title_full_unstemmed MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma
title_short MAEL as a diagnostic marker for the early detection of esophageal squamous cell carcinoma
title_sort mael as a diagnostic marker for the early detection of esophageal squamous cell carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077922/
https://www.ncbi.nlm.nih.gov/pubmed/33902648
http://dx.doi.org/10.1186/s13000-021-01098-z
work_keys_str_mv AT abbaszadeganmohammadreza maelasadiagnosticmarkerfortheearlydetectionofesophagealsquamouscellcarcinoma
AT taghehchiannegin maelasadiagnosticmarkerfortheearlydetectionofesophagealsquamouscellcarcinoma
AT aarabiazadeh maelasadiagnosticmarkerfortheearlydetectionofesophagealsquamouscellcarcinoma
AT akbarifaride maelasadiagnosticmarkerfortheearlydetectionofesophagealsquamouscellcarcinoma
AT saburiehsan maelasadiagnosticmarkerfortheearlydetectionofesophagealsquamouscellcarcinoma
AT moghbelimeysam maelasadiagnosticmarkerfortheearlydetectionofesophagealsquamouscellcarcinoma