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Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization

The study of cellular migration dynamics and strategies plays a relevant role in the understanding of both physiological and pathological processes. An important example could be the link between cancer cell motility and tumor evolution into metastatic stage. These strategies can be strongly influen...

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Autores principales: Sala, Federico, Ficorella, Carlotta, Martínez Vázquez, Rebeca, Eichholz, Hannah Marie, Käs, Josef A., Osellame, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078855/
https://www.ncbi.nlm.nih.gov/pubmed/33928074
http://dx.doi.org/10.3389/fbioe.2021.664094
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author Sala, Federico
Ficorella, Carlotta
Martínez Vázquez, Rebeca
Eichholz, Hannah Marie
Käs, Josef A.
Osellame, Roberto
author_facet Sala, Federico
Ficorella, Carlotta
Martínez Vázquez, Rebeca
Eichholz, Hannah Marie
Käs, Josef A.
Osellame, Roberto
author_sort Sala, Federico
collection PubMed
description The study of cellular migration dynamics and strategies plays a relevant role in the understanding of both physiological and pathological processes. An important example could be the link between cancer cell motility and tumor evolution into metastatic stage. These strategies can be strongly influenced by the extracellular environment and the consequent mechanical constrains. In this framework, the possibility to study the behavior of single cells when subject to specific topological constraints could be an important tool in the hands of biologists. Two-photon polymerization is a sub-micrometric additive manufacturing technique that allows the fabrication of 3D structures in biocompatible resins, enabling the realization of ad hoc biochips for cell motility analyses, providing different types of mechanical stimuli. In our work, we present a new strategy for the realization of multilayer microfluidic lab-on-a-chip constructs for the study of cell motility which guarantees complete optical accessibility and the possibility to freely shape the migration area, to tailor it to the requirements of the specific cell type or experiment. The device includes a series of micro-constrictions that induce different types of mechanical stress on the cells during their migration. We show the realization of different possible geometries, in order to prove the versatility of the technique. As a proof of concept, we present the use of one of these devices for the study of the motility of murine neuronal cancer cells under high physical confinement, highlighting their peculiar migration mechanisms.
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spelling pubmed-80788552021-04-28 Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization Sala, Federico Ficorella, Carlotta Martínez Vázquez, Rebeca Eichholz, Hannah Marie Käs, Josef A. Osellame, Roberto Front Bioeng Biotechnol Bioengineering and Biotechnology The study of cellular migration dynamics and strategies plays a relevant role in the understanding of both physiological and pathological processes. An important example could be the link between cancer cell motility and tumor evolution into metastatic stage. These strategies can be strongly influenced by the extracellular environment and the consequent mechanical constrains. In this framework, the possibility to study the behavior of single cells when subject to specific topological constraints could be an important tool in the hands of biologists. Two-photon polymerization is a sub-micrometric additive manufacturing technique that allows the fabrication of 3D structures in biocompatible resins, enabling the realization of ad hoc biochips for cell motility analyses, providing different types of mechanical stimuli. In our work, we present a new strategy for the realization of multilayer microfluidic lab-on-a-chip constructs for the study of cell motility which guarantees complete optical accessibility and the possibility to freely shape the migration area, to tailor it to the requirements of the specific cell type or experiment. The device includes a series of micro-constrictions that induce different types of mechanical stress on the cells during their migration. We show the realization of different possible geometries, in order to prove the versatility of the technique. As a proof of concept, we present the use of one of these devices for the study of the motility of murine neuronal cancer cells under high physical confinement, highlighting their peculiar migration mechanisms. Frontiers Media S.A. 2021-04-13 /pmc/articles/PMC8078855/ /pubmed/33928074 http://dx.doi.org/10.3389/fbioe.2021.664094 Text en Copyright © 2021 Sala, Ficorella, Martínez Vázquez, Eichholz, Käs and Osellame. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Sala, Federico
Ficorella, Carlotta
Martínez Vázquez, Rebeca
Eichholz, Hannah Marie
Käs, Josef A.
Osellame, Roberto
Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization
title Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization
title_full Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization
title_fullStr Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization
title_full_unstemmed Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization
title_short Rapid Prototyping of 3D Biochips for Cell Motility Studies Using Two-Photon Polymerization
title_sort rapid prototyping of 3d biochips for cell motility studies using two-photon polymerization
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078855/
https://www.ncbi.nlm.nih.gov/pubmed/33928074
http://dx.doi.org/10.3389/fbioe.2021.664094
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