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Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean

Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Studies based on different isolates and populations have already been carried out to elucidate the genetic mechanisms of resistance to ALS. However, understanding of the interaction of this resistance with t...

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Autores principales: de Almeida, Caléo Panhoca, de Carvalho Paulino, Jean Fausto, Bonfante, Gabriel Francesco Janini, Perseguini, Juliana Morini Kupper Cardoso, Santos, Isabella Laporte, Gonçalves, João Guilherme Ribeiro, Patrício, Flávia Rodrigues Alves, Taniguti, Cristiane Hayumi, Gesteira, Gabriel de Siqueira, Garcia, Antônio Augusto Franco, Song, Qijian, Carbonell, Sérgio Augusto Morais, Chiorato, Alisson Fernando, Benchimol-Reis, Luciana Lasry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078856/
https://www.ncbi.nlm.nih.gov/pubmed/33927738
http://dx.doi.org/10.3389/fpls.2021.647043
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author de Almeida, Caléo Panhoca
de Carvalho Paulino, Jean Fausto
Bonfante, Gabriel Francesco Janini
Perseguini, Juliana Morini Kupper Cardoso
Santos, Isabella Laporte
Gonçalves, João Guilherme Ribeiro
Patrício, Flávia Rodrigues Alves
Taniguti, Cristiane Hayumi
Gesteira, Gabriel de Siqueira
Garcia, Antônio Augusto Franco
Song, Qijian
Carbonell, Sérgio Augusto Morais
Chiorato, Alisson Fernando
Benchimol-Reis, Luciana Lasry
author_facet de Almeida, Caléo Panhoca
de Carvalho Paulino, Jean Fausto
Bonfante, Gabriel Francesco Janini
Perseguini, Juliana Morini Kupper Cardoso
Santos, Isabella Laporte
Gonçalves, João Guilherme Ribeiro
Patrício, Flávia Rodrigues Alves
Taniguti, Cristiane Hayumi
Gesteira, Gabriel de Siqueira
Garcia, Antônio Augusto Franco
Song, Qijian
Carbonell, Sérgio Augusto Morais
Chiorato, Alisson Fernando
Benchimol-Reis, Luciana Lasry
author_sort de Almeida, Caléo Panhoca
collection PubMed
description Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Studies based on different isolates and populations have already been carried out to elucidate the genetic mechanisms of resistance to ALS. However, understanding of the interaction of this resistance with the reproductive stages of common bean is lacking. The aim of the present study was to identify ALS resistance loci at different plant growth stages (PGS) by association and linkage mapping approaches. An BC(2)F(3) inter-gene pool cross population (AND 277 × IAC-Milênio – AM population) profiled with 1,091 SNPs from genotyping by sequencing (GBS) was used for linkage mapping, and a carioca diversity panel (CDP) genotyped by 5,398 SNPs from BeadChip assay technology was used for association mapping. Both populations were evaluated for ALS resistance at the V2 and V3 PGSs (controlled conditions) and R8 PGS (field conditions). Different QTL (quantitative trait loci) were detected for the three PGSs and both populations, showing a different quantitative profile of the disease at different plant growth stages. For the three PGS, multiple interval mapping (MIM) identified seven significant QTL, and the Genome-wide association study (GWAS) identified fourteen associate SNPs. Several loci validated regions of previous studies, and Phg-1, Phg-2, Phg-4, and Phg-5, among the 5 loci of greatest effects reported in the literature, were detected in the CDP. The AND 277 cultivar contained both the Phg-1 and the Phg-5 QTL, which is reported for the first time in the descendant cultivar CAL143 as ALS10.1(UC). The novel QTL named ALS11.1(AM) was located at the beginning of chromosome Pv11. Gene annotation revealed several putative resistance genes involved in the ALS response at the three PGSs, and with the markers and loci identified, new specific molecular markers can be developed, representing a powerful tool for common bean crop improvement and for gain in ALS resistance.
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spelling pubmed-80788562021-04-28 Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean de Almeida, Caléo Panhoca de Carvalho Paulino, Jean Fausto Bonfante, Gabriel Francesco Janini Perseguini, Juliana Morini Kupper Cardoso Santos, Isabella Laporte Gonçalves, João Guilherme Ribeiro Patrício, Flávia Rodrigues Alves Taniguti, Cristiane Hayumi Gesteira, Gabriel de Siqueira Garcia, Antônio Augusto Franco Song, Qijian Carbonell, Sérgio Augusto Morais Chiorato, Alisson Fernando Benchimol-Reis, Luciana Lasry Front Plant Sci Plant Science Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Studies based on different isolates and populations have already been carried out to elucidate the genetic mechanisms of resistance to ALS. However, understanding of the interaction of this resistance with the reproductive stages of common bean is lacking. The aim of the present study was to identify ALS resistance loci at different plant growth stages (PGS) by association and linkage mapping approaches. An BC(2)F(3) inter-gene pool cross population (AND 277 × IAC-Milênio – AM population) profiled with 1,091 SNPs from genotyping by sequencing (GBS) was used for linkage mapping, and a carioca diversity panel (CDP) genotyped by 5,398 SNPs from BeadChip assay technology was used for association mapping. Both populations were evaluated for ALS resistance at the V2 and V3 PGSs (controlled conditions) and R8 PGS (field conditions). Different QTL (quantitative trait loci) were detected for the three PGSs and both populations, showing a different quantitative profile of the disease at different plant growth stages. For the three PGS, multiple interval mapping (MIM) identified seven significant QTL, and the Genome-wide association study (GWAS) identified fourteen associate SNPs. Several loci validated regions of previous studies, and Phg-1, Phg-2, Phg-4, and Phg-5, among the 5 loci of greatest effects reported in the literature, were detected in the CDP. The AND 277 cultivar contained both the Phg-1 and the Phg-5 QTL, which is reported for the first time in the descendant cultivar CAL143 as ALS10.1(UC). The novel QTL named ALS11.1(AM) was located at the beginning of chromosome Pv11. Gene annotation revealed several putative resistance genes involved in the ALS response at the three PGSs, and with the markers and loci identified, new specific molecular markers can be developed, representing a powerful tool for common bean crop improvement and for gain in ALS resistance. Frontiers Media S.A. 2021-04-13 /pmc/articles/PMC8078856/ /pubmed/33927738 http://dx.doi.org/10.3389/fpls.2021.647043 Text en Copyright © 2021 Almeida, de Carvalho Paulino, Bonfante, Perseguini, Santos, Gonçalves, Patrício, Taniguti, Gesteira, Garcia, Song, Carbonell, Chiorato and Benchimol-Reis. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
de Almeida, Caléo Panhoca
de Carvalho Paulino, Jean Fausto
Bonfante, Gabriel Francesco Janini
Perseguini, Juliana Morini Kupper Cardoso
Santos, Isabella Laporte
Gonçalves, João Guilherme Ribeiro
Patrício, Flávia Rodrigues Alves
Taniguti, Cristiane Hayumi
Gesteira, Gabriel de Siqueira
Garcia, Antônio Augusto Franco
Song, Qijian
Carbonell, Sérgio Augusto Morais
Chiorato, Alisson Fernando
Benchimol-Reis, Luciana Lasry
Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean
title Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean
title_full Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean
title_fullStr Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean
title_full_unstemmed Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean
title_short Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean
title_sort angular leaf spot resistance loci associated with different plant growth stages in common bean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078856/
https://www.ncbi.nlm.nih.gov/pubmed/33927738
http://dx.doi.org/10.3389/fpls.2021.647043
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