Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences

Complete plastid genome (plastome) and ribosomal DNA (rDNA) sequences of three Rubus accessions (two Rubus longisepalus and one R. hirsutus) were newly assembled using Illumina whole-genome sequences. Rubus longisepalus Nakai and R. longisepalus var. tozawai, described as different varieties, have i...

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Autores principales: Park, Young Sang, Park, Jee Young, Kang, Jung Hwa, Lee, Wan Hee, Yang, Tae-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079122/
https://www.ncbi.nlm.nih.gov/pubmed/33969195
http://dx.doi.org/10.1080/23802359.2021.1911712
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author Park, Young Sang
Park, Jee Young
Kang, Jung Hwa
Lee, Wan Hee
Yang, Tae-Jin
author_facet Park, Young Sang
Park, Jee Young
Kang, Jung Hwa
Lee, Wan Hee
Yang, Tae-Jin
author_sort Park, Young Sang
collection PubMed
description Complete plastid genome (plastome) and ribosomal DNA (rDNA) sequences of three Rubus accessions (two Rubus longisepalus and one R. hirsutus) were newly assembled using Illumina whole-genome sequences. Rubus longisepalus Nakai and R. longisepalus var. tozawai, described as different varieties, have identical plastomes and rDNA sequences. The plastomes are 155,957 bp and 156,005 bp and the 45S rDNA transcription unit sizes are 5809 bp and 5811 bp in R. longisepalus and R. hirsutus, respectively. The 5S rDNA transcription unit is an identical 121 bp in three Rubus accessions. We developed three DNA markers to authenticate R. longisepalus and R. hirsutus based on plastome diversity. Phylogenomic analysis revealed that the Rubus species classified as two clades and R. longisepalus, R. hirsutus, and R. chingii are the most closely related species in clade 1.
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spelling pubmed-80791222021-05-06 Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences Park, Young Sang Park, Jee Young Kang, Jung Hwa Lee, Wan Hee Yang, Tae-Jin Mitochondrial DNA B Resour Rapid Communication Complete plastid genome (plastome) and ribosomal DNA (rDNA) sequences of three Rubus accessions (two Rubus longisepalus and one R. hirsutus) were newly assembled using Illumina whole-genome sequences. Rubus longisepalus Nakai and R. longisepalus var. tozawai, described as different varieties, have identical plastomes and rDNA sequences. The plastomes are 155,957 bp and 156,005 bp and the 45S rDNA transcription unit sizes are 5809 bp and 5811 bp in R. longisepalus and R. hirsutus, respectively. The 5S rDNA transcription unit is an identical 121 bp in three Rubus accessions. We developed three DNA markers to authenticate R. longisepalus and R. hirsutus based on plastome diversity. Phylogenomic analysis revealed that the Rubus species classified as two clades and R. longisepalus, R. hirsutus, and R. chingii are the most closely related species in clade 1. Taylor & Francis 2021-04-20 /pmc/articles/PMC8079122/ /pubmed/33969195 http://dx.doi.org/10.1080/23802359.2021.1911712 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Rapid Communication
Park, Young Sang
Park, Jee Young
Kang, Jung Hwa
Lee, Wan Hee
Yang, Tae-Jin
Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences
title Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences
title_full Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences
title_fullStr Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences
title_full_unstemmed Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences
title_short Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences
title_sort diversity and authentication of rubus accessions revealed by complete plastid genome and rdna sequences
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079122/
https://www.ncbi.nlm.nih.gov/pubmed/33969195
http://dx.doi.org/10.1080/23802359.2021.1911712
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